Anterior cruciate ligament (ACL) reconstruction rates are rising, particularly among female athletes, though causes remain unclear. This study: (i) identify accurate machine learning models to predict ACL length, strain, and force during six high-impact and daily activities; (ii) assess the significance of kinematic and constitutional parameters; and (iii) analyse gender-based injury risk patterns. Using 9,375 observations per variable, 42 models were trained. Cubist, Generalized Boosted Models (GBM), and Random Forest (RF) achieved the best R2, RMSE, and MAE. Knee flexion and external rotation strongly predicted ACL strain and force. Female athletes showed higher rotation during cuts, elevating ACL strain and risk.