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Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates

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Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates. / Munir, Muhammad; Zohari, Siamak; Abbas, Muhammad et al.
In: Indian Journal of Virology, Vol. 24, No. 3, 01.12.2013, p. 342-348.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Munir, M, Zohari, S, Abbas, M, Shabbir, MZ, Zahid, MN, Latif, MS, Iqbal, M, Ahsan Qureshi, ZU & Berg, M 2013, 'Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates', Indian Journal of Virology, vol. 24, no. 3, pp. 342-348. https://doi.org/10.1007/s13337-013-0144-1

APA

Munir, M., Zohari, S., Abbas, M., Shabbir, M. Z., Zahid, M. N., Latif, M. S., Iqbal, M., Ahsan Qureshi, Z. U., & Berg, M. (2013). Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates. Indian Journal of Virology, 24(3), 342-348. https://doi.org/10.1007/s13337-013-0144-1

Vancouver

Munir M, Zohari S, Abbas M, Shabbir MZ, Zahid MN, Latif MS et al. Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates. Indian Journal of Virology. 2013 Dec 1;24(3):342-348. doi: 10.1007/s13337-013-0144-1

Author

Munir, Muhammad ; Zohari, Siamak ; Abbas, Muhammad et al. / Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates. In: Indian Journal of Virology. 2013 ; Vol. 24, No. 3. pp. 342-348.

Bibtex

@article{9a221b2143cf459a8d72aab8dda6f68a,
title = "Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates",
abstract = "Several outbreaks of avian influenza (AI) caused by H9N2 subtype, have been reported in the poultry industry during 1990 around the globe. Currently, H9N2 are endemic in the large area of Middle and Far East, including Pakistan. Since H9N2 AI viruses are sporadically reported from humans, extensive incidence of H9N2 in poultry imposes a great risk for human health. In this context, continuous monitoring of the poultry and determining the genetic nature of these viruses are fundamental to predict any future threat. Thus gene sequences of one isolate of H9N2, isolated from commercial poultry flocks, were analyzed. The results of this investigation, based on hemagglutinin (HA), neuraminidase (NA) and nonstructural genes, showed that Pakistani H9N2 isolates are closely related to each other and to other H9N2 isolates from the Middle East. However, several unusual substitutions with unknown functional consequences were observed in HA and NA proteins and thus warrant further investigations for their possible role in viral biology. In conclusion, these findings provide information regarding the genetic nature of H9N2 avian influenza viruses in Pakistani poultry and necessitate the sequencing of more H9N2 viruses from both naturally infected and vaccinated flocks.",
keywords = "DNA sequencing, H9N2, Influenza, Pakistan, Phylogenetic analysis",
author = "Muhammad Munir and Siamak Zohari and Muhammad Abbas and Shabbir, {Muhammad Zubair} and Zahid, {Muhammad Nauman} and Latif, {Muhammad Saqib} and Muhammad Iqbal and {Ahsan Qureshi}, {Zafar Ul} and Mikael Berg",
year = "2013",
month = dec,
day = "1",
doi = "10.1007/s13337-013-0144-1",
language = "English",
volume = "24",
pages = "342--348",
journal = "Indian Journal of Virology",
issn = "0970-2822",
publisher = "Springer India",
number = "3",

}

RIS

TY - JOUR

T1 - Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates

AU - Munir, Muhammad

AU - Zohari, Siamak

AU - Abbas, Muhammad

AU - Shabbir, Muhammad Zubair

AU - Zahid, Muhammad Nauman

AU - Latif, Muhammad Saqib

AU - Iqbal, Muhammad

AU - Ahsan Qureshi, Zafar Ul

AU - Berg, Mikael

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Several outbreaks of avian influenza (AI) caused by H9N2 subtype, have been reported in the poultry industry during 1990 around the globe. Currently, H9N2 are endemic in the large area of Middle and Far East, including Pakistan. Since H9N2 AI viruses are sporadically reported from humans, extensive incidence of H9N2 in poultry imposes a great risk for human health. In this context, continuous monitoring of the poultry and determining the genetic nature of these viruses are fundamental to predict any future threat. Thus gene sequences of one isolate of H9N2, isolated from commercial poultry flocks, were analyzed. The results of this investigation, based on hemagglutinin (HA), neuraminidase (NA) and nonstructural genes, showed that Pakistani H9N2 isolates are closely related to each other and to other H9N2 isolates from the Middle East. However, several unusual substitutions with unknown functional consequences were observed in HA and NA proteins and thus warrant further investigations for their possible role in viral biology. In conclusion, these findings provide information regarding the genetic nature of H9N2 avian influenza viruses in Pakistani poultry and necessitate the sequencing of more H9N2 viruses from both naturally infected and vaccinated flocks.

AB - Several outbreaks of avian influenza (AI) caused by H9N2 subtype, have been reported in the poultry industry during 1990 around the globe. Currently, H9N2 are endemic in the large area of Middle and Far East, including Pakistan. Since H9N2 AI viruses are sporadically reported from humans, extensive incidence of H9N2 in poultry imposes a great risk for human health. In this context, continuous monitoring of the poultry and determining the genetic nature of these viruses are fundamental to predict any future threat. Thus gene sequences of one isolate of H9N2, isolated from commercial poultry flocks, were analyzed. The results of this investigation, based on hemagglutinin (HA), neuraminidase (NA) and nonstructural genes, showed that Pakistani H9N2 isolates are closely related to each other and to other H9N2 isolates from the Middle East. However, several unusual substitutions with unknown functional consequences were observed in HA and NA proteins and thus warrant further investigations for their possible role in viral biology. In conclusion, these findings provide information regarding the genetic nature of H9N2 avian influenza viruses in Pakistani poultry and necessitate the sequencing of more H9N2 viruses from both naturally infected and vaccinated flocks.

KW - DNA sequencing

KW - H9N2

KW - Influenza

KW - Pakistan

KW - Phylogenetic analysis

U2 - 10.1007/s13337-013-0144-1

DO - 10.1007/s13337-013-0144-1

M3 - Journal article

AN - SCOPUS:84892815834

VL - 24

SP - 342

EP - 348

JO - Indian Journal of Virology

JF - Indian Journal of Virology

SN - 0970-2822

IS - 3

ER -