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Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration

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Publication date19/08/2017
Host publicationPhotorespiration
EditorsA. Fernie, H. Bauwe, A. Weber
Place of PublicationNew York
PublisherHumana Press
Pages243-270
Number of pages28
ISBN (Print)9781493972241
<mark>Original language</mark>English

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1653

Abstract

High-resolution images obtained from plant tissues processed for light microscopy, transmission electron microscopy, and immunohistochemistry have provided crucial links between plant subcellular structure and physiology during photorespiration as well as the impact of photorespiration on plant evolution and development. This chapter presents established protocols to guide researchers in the preparation of plant tissues for high-resolution imaging with a light and transmission electron microscope and detection of proteins using immunohistochemistry. Discussion of concepts and theory behind each step in the process from tissue preservation to staining of resin-embedded tissues is included to enhance the understanding of all steps in the procedure. We also include a brief protocol for quantification of cellular parameters from high-resolution images to help researchers rigorously test hypotheses.