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Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration

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Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration. / Khoshravesh, Roxana; Lundsgaard-Nielsen, Vanessa; Sultmanis, Stefanie et al.
Photorespiration. ed. / A. Fernie; H. Bauwe; A. Weber. New York: Humana Press, 2017. p. 243-270 (Methods in Molecular Biology; Vol. 1653).

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter

Harvard

Khoshravesh, R, Lundsgaard-Nielsen, V, Sultmanis, S & Sage, TL 2017, Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration. in A Fernie, H Bauwe & A Weber (eds), Photorespiration. Methods in Molecular Biology, vol. 1653, Humana Press, New York, pp. 243-270. https://doi.org/10.1007/978-1-4939-7225-8_17

APA

Khoshravesh, R., Lundsgaard-Nielsen, V., Sultmanis, S., & Sage, T. L. (2017). Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration. In A. Fernie, H. Bauwe, & A. Weber (Eds.), Photorespiration (pp. 243-270). (Methods in Molecular Biology; Vol. 1653). Humana Press. https://doi.org/10.1007/978-1-4939-7225-8_17

Vancouver

Khoshravesh R, Lundsgaard-Nielsen V, Sultmanis S, Sage TL. Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration. In Fernie A, Bauwe H, Weber A, editors, Photorespiration. New York: Humana Press. 2017. p. 243-270. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-7225-8_17

Author

Khoshravesh, Roxana ; Lundsgaard-Nielsen, Vanessa ; Sultmanis, Stefanie et al. / Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration. Photorespiration. editor / A. Fernie ; H. Bauwe ; A. Weber. New York : Humana Press, 2017. pp. 243-270 (Methods in Molecular Biology).

Bibtex

@inbook{242b982393c544948ebbc41cbcd0f5b2,
title = "Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration",
abstract = "High-resolution images obtained from plant tissues processed for light microscopy, transmission electron microscopy, and immunohistochemistry have provided crucial links between plant subcellular structure and physiology during photorespiration as well as the impact of photorespiration on plant evolution and development. This chapter presents established protocols to guide researchers in the preparation of plant tissues for high-resolution imaging with a light and transmission electron microscope and detection of proteins using immunohistochemistry. Discussion of concepts and theory behind each step in the process from tissue preservation to staining of resin-embedded tissues is included to enhance the understanding of all steps in the procedure. We also include a brief protocol for quantification of cellular parameters from high-resolution images to help researchers rigorously test hypotheses.",
keywords = "Light microscopy, Transmission electron microscopy, High-resolution microscopy, Immunohistochemistry, Photorespiration, Chloroplasts, Mitochondria, Peroxisomes",
author = "Roxana Khoshravesh and Vanessa Lundsgaard-Nielsen and Stefanie Sultmanis and Sage, {Tammy L.}",
year = "2017",
month = aug,
day = "19",
doi = "10.1007/978-1-4939-7225-8_17",
language = "English",
isbn = "9781493972241",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "243--270",
editor = "A. Fernie and H. Bauwe and A. Weber",
booktitle = "Photorespiration",

}

RIS

TY - CHAP

T1 - Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration

AU - Khoshravesh, Roxana

AU - Lundsgaard-Nielsen, Vanessa

AU - Sultmanis, Stefanie

AU - Sage, Tammy L.

PY - 2017/8/19

Y1 - 2017/8/19

N2 - High-resolution images obtained from plant tissues processed for light microscopy, transmission electron microscopy, and immunohistochemistry have provided crucial links between plant subcellular structure and physiology during photorespiration as well as the impact of photorespiration on plant evolution and development. This chapter presents established protocols to guide researchers in the preparation of plant tissues for high-resolution imaging with a light and transmission electron microscope and detection of proteins using immunohistochemistry. Discussion of concepts and theory behind each step in the process from tissue preservation to staining of resin-embedded tissues is included to enhance the understanding of all steps in the procedure. We also include a brief protocol for quantification of cellular parameters from high-resolution images to help researchers rigorously test hypotheses.

AB - High-resolution images obtained from plant tissues processed for light microscopy, transmission electron microscopy, and immunohistochemistry have provided crucial links between plant subcellular structure and physiology during photorespiration as well as the impact of photorespiration on plant evolution and development. This chapter presents established protocols to guide researchers in the preparation of plant tissues for high-resolution imaging with a light and transmission electron microscope and detection of proteins using immunohistochemistry. Discussion of concepts and theory behind each step in the process from tissue preservation to staining of resin-embedded tissues is included to enhance the understanding of all steps in the procedure. We also include a brief protocol for quantification of cellular parameters from high-resolution images to help researchers rigorously test hypotheses.

KW - Light microscopy

KW - Transmission electron microscopy

KW - High-resolution microscopy

KW - Immunohistochemistry

KW - Photorespiration

KW - Chloroplasts

KW - Mitochondria

KW - Peroxisomes

U2 - 10.1007/978-1-4939-7225-8_17

DO - 10.1007/978-1-4939-7225-8_17

M3 - Chapter

SN - 9781493972241

T3 - Methods in Molecular Biology

SP - 243

EP - 270

BT - Photorespiration

A2 - Fernie, A.

A2 - Bauwe, H.

A2 - Weber, A.

PB - Humana Press

CY - New York

ER -