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Macrophage origin limits functional plasticity in helminth-bacterial co-infection

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Macrophage origin limits functional plasticity in helminth-bacterial co-infection. / Rückerl, Dominik; Campbell, Sharon M; Duncan, Sheelagh et al.
In: PLoS Pathogens, Vol. 13, No. 3, e1006233, 23.03.2017.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Rückerl, D, Campbell, SM, Duncan, S, Sutherland, TE, Jenkins, SJ, Hewitson, JP, Barr, TA, Jackson-Jones, LH, Maizels, RM & Allen, JE 2017, 'Macrophage origin limits functional plasticity in helminth-bacterial co-infection', PLoS Pathogens, vol. 13, no. 3, e1006233. https://doi.org/10.1371/journal.ppat.1006233

APA

Rückerl, D., Campbell, S. M., Duncan, S., Sutherland, T. E., Jenkins, S. J., Hewitson, J. P., Barr, T. A., Jackson-Jones, L. H., Maizels, R. M., & Allen, J. E. (2017). Macrophage origin limits functional plasticity in helminth-bacterial co-infection. PLoS Pathogens, 13(3), Article e1006233. https://doi.org/10.1371/journal.ppat.1006233

Vancouver

Rückerl D, Campbell SM, Duncan S, Sutherland TE, Jenkins SJ, Hewitson JP et al. Macrophage origin limits functional plasticity in helminth-bacterial co-infection. PLoS Pathogens. 2017 Mar 23;13(3):e1006233. doi: 10.1371/journal.ppat.1006233

Author

Rückerl, Dominik ; Campbell, Sharon M ; Duncan, Sheelagh et al. / Macrophage origin limits functional plasticity in helminth-bacterial co-infection. In: PLoS Pathogens. 2017 ; Vol. 13, No. 3.

Bibtex

@article{6f6194a2abbd4d91890335f7b7cb8404,
title = "Macrophage origin limits functional plasticity in helminth-bacterial co-infection",
abstract = "Rapid reprogramming of the macrophage activation phenotype is considered important in the defense against consecutive infection with diverse infectious agents. However, in the setting of persistent, chronic infection the functional importance of macrophage-intrinsic adaptation to changing environments vs. recruitment of new macrophages remains unclear. Here we show that resident peritoneal macrophages expanded by infection with the nematode Heligmosomoides polygyrus bakeri altered their activation phenotype in response to infection with Salmonella enterica ser. Typhimurium in vitro and in vivo. The nematode-expanded resident F4/80high macrophages efficiently upregulated bacterial induced effector molecules (e.g. MHC-II, NOS2) similarly to newly recruited monocyte-derived macrophages. Nonetheless, recruitment of blood monocyte-derived macrophages to Salmonella infection occurred with equal magnitude in co-infected animals and caused displacement of the nematode-expanded, tissue resident-derived macrophages from the peritoneal cavity. Global gene expression analysis revealed that although nematode-expanded resident F4/80high macrophages made an anti-bacterial response, this was muted as compared to newly recruited F4/80low macrophages. However, the F4/80high macrophages adopted unique functional characteristics that included enhanced neutrophil-stimulating chemokine production. Thus, our data provide important evidence that plastic adaptation of MΦ activation does occur in vivo, but that cellular plasticity is outweighed by functional capabilities specific to the tissue origin of the cell.",
keywords = "Animals, Coinfection, Flow Cytometry, Macrophage Activation, Macrophages, Mice, Mice, Inbred C57BL, Nematospiroides dubius, Oligonucleotide Array Sequence Analysis, Salmonella Infections, Animal, Salmonella typhi, Strongylida Infections, Journal Article",
author = "Dominik R{\"u}ckerl and Campbell, {Sharon M} and Sheelagh Duncan and Sutherland, {Tara E} and Jenkins, {Stephen J} and Hewitson, {James P} and Barr, {Tom A} and Jackson-Jones, {Lucy H} and Maizels, {Rick M} and Allen, {Judith E}",
year = "2017",
month = mar,
day = "23",
doi = "10.1371/journal.ppat.1006233",
language = "English",
volume = "13",
journal = "PLoS Pathogens",
issn = "1553-7366",
publisher = "Public Library of Science",
number = "3",

}

RIS

TY - JOUR

T1 - Macrophage origin limits functional plasticity in helminth-bacterial co-infection

AU - Rückerl, Dominik

AU - Campbell, Sharon M

AU - Duncan, Sheelagh

AU - Sutherland, Tara E

AU - Jenkins, Stephen J

AU - Hewitson, James P

AU - Barr, Tom A

AU - Jackson-Jones, Lucy H

AU - Maizels, Rick M

AU - Allen, Judith E

PY - 2017/3/23

Y1 - 2017/3/23

N2 - Rapid reprogramming of the macrophage activation phenotype is considered important in the defense against consecutive infection with diverse infectious agents. However, in the setting of persistent, chronic infection the functional importance of macrophage-intrinsic adaptation to changing environments vs. recruitment of new macrophages remains unclear. Here we show that resident peritoneal macrophages expanded by infection with the nematode Heligmosomoides polygyrus bakeri altered their activation phenotype in response to infection with Salmonella enterica ser. Typhimurium in vitro and in vivo. The nematode-expanded resident F4/80high macrophages efficiently upregulated bacterial induced effector molecules (e.g. MHC-II, NOS2) similarly to newly recruited monocyte-derived macrophages. Nonetheless, recruitment of blood monocyte-derived macrophages to Salmonella infection occurred with equal magnitude in co-infected animals and caused displacement of the nematode-expanded, tissue resident-derived macrophages from the peritoneal cavity. Global gene expression analysis revealed that although nematode-expanded resident F4/80high macrophages made an anti-bacterial response, this was muted as compared to newly recruited F4/80low macrophages. However, the F4/80high macrophages adopted unique functional characteristics that included enhanced neutrophil-stimulating chemokine production. Thus, our data provide important evidence that plastic adaptation of MΦ activation does occur in vivo, but that cellular plasticity is outweighed by functional capabilities specific to the tissue origin of the cell.

AB - Rapid reprogramming of the macrophage activation phenotype is considered important in the defense against consecutive infection with diverse infectious agents. However, in the setting of persistent, chronic infection the functional importance of macrophage-intrinsic adaptation to changing environments vs. recruitment of new macrophages remains unclear. Here we show that resident peritoneal macrophages expanded by infection with the nematode Heligmosomoides polygyrus bakeri altered their activation phenotype in response to infection with Salmonella enterica ser. Typhimurium in vitro and in vivo. The nematode-expanded resident F4/80high macrophages efficiently upregulated bacterial induced effector molecules (e.g. MHC-II, NOS2) similarly to newly recruited monocyte-derived macrophages. Nonetheless, recruitment of blood monocyte-derived macrophages to Salmonella infection occurred with equal magnitude in co-infected animals and caused displacement of the nematode-expanded, tissue resident-derived macrophages from the peritoneal cavity. Global gene expression analysis revealed that although nematode-expanded resident F4/80high macrophages made an anti-bacterial response, this was muted as compared to newly recruited F4/80low macrophages. However, the F4/80high macrophages adopted unique functional characteristics that included enhanced neutrophil-stimulating chemokine production. Thus, our data provide important evidence that plastic adaptation of MΦ activation does occur in vivo, but that cellular plasticity is outweighed by functional capabilities specific to the tissue origin of the cell.

KW - Animals

KW - Coinfection

KW - Flow Cytometry

KW - Macrophage Activation

KW - Macrophages

KW - Mice

KW - Mice, Inbred C57BL

KW - Nematospiroides dubius

KW - Oligonucleotide Array Sequence Analysis

KW - Salmonella Infections, Animal

KW - Salmonella typhi

KW - Strongylida Infections

KW - Journal Article

U2 - 10.1371/journal.ppat.1006233

DO - 10.1371/journal.ppat.1006233

M3 - Journal article

C2 - 28334040

VL - 13

JO - PLoS Pathogens

JF - PLoS Pathogens

SN - 1553-7366

IS - 3

M1 - e1006233

ER -