Membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry

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Membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry. / Porter, J.; Pickup, R.; Edwards, C.
In: FEMS Microbiology Letters, Vol. 132, No. 3, 15.10.1995, p. 259-262.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Porter, J , Pickup, R & Edwards, C 1995, 'Membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry', FEMS Microbiology Letters, vol. 132, no. 3, pp. 259-262. https://doi.org/10.1016/0378-1097(95)00320-5

APA

Porter, J., Pickup, R., & Edwards, C. (1995). Membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry. FEMS Microbiology Letters, 132(3), 259-262. https://doi.org/10.1016/0378-1097(95)00320-5

Vancouver

Porter J , Pickup R , Edwards C. Membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry. FEMS Microbiology Letters. 1995 Oct 15;132(3):259-262. doi: 10.1016/0378-1097(95)00320-5

Author

Porter, J. ; Pickup, R. ; Edwards, C. / Membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry. In: FEMS Microbiology Letters. 1995 ; Vol. 132, No. 3. pp. 259-262.

Bibtex

@article{f4f1030ae6894a0d9efc67a06b365a8a,

title = "Membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry",

abstract = "The ionophore, valinomycin, was investigated as a possible means of bacterial viability assessment using the fluorescent membrane potential dye rhodamine 123. Membrane hyperpolarisation in Escherichia coli, Pseudomonas fluorescent, Enterobacter aerogenes and Arthrobacter globiformis was examined during exponential growth and during stress by brief starvation in a high sodium, low potassium buffer using flow cytometric analysis of rhodamine 123 uptake. Dye uptake was variable both between species and amongst cells from the same culture. Exponential phase cells showed no increase in dye uptake due to valinomycin treatment. Stressed P. fluorescens cells responded to valinomycin treatment by increased dye uptake, while stressed E. coli and A. globiformis cells showed no response. Approximately 50% of stressed Eb. aerogenes cells responded to valinomycin. The results demonstrate the limitations of rhodamine dye for viability analysing the viability of diverse bacterial communities and underline the degree of cell heterogeneity in batch cultures.",

keywords = "Heterogeneity, Membrane potential, Stressed bacteria, Valinomycin",

author = "J. Porter and R. Pickup and C. Edwards",

year = "1995",

month = oct,

day = "15",

doi = "10.1016/0378-1097(95)00320-5",

language = "English",

volume = "132",

pages = "259--262",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "3",

}

RIS

TY - JOUR

T1 - Membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry

AU - Porter, J.

AU - Pickup, R.

AU - Edwards, C.

PY - 1995/10/15

Y1 - 1995/10/15

N2 - The ionophore, valinomycin, was investigated as a possible means of bacterial viability assessment using the fluorescent membrane potential dye rhodamine 123. Membrane hyperpolarisation in Escherichia coli, Pseudomonas fluorescent, Enterobacter aerogenes and Arthrobacter globiformis was examined during exponential growth and during stress by brief starvation in a high sodium, low potassium buffer using flow cytometric analysis of rhodamine 123 uptake. Dye uptake was variable both between species and amongst cells from the same culture. Exponential phase cells showed no increase in dye uptake due to valinomycin treatment. Stressed P. fluorescens cells responded to valinomycin treatment by increased dye uptake, while stressed E. coli and A. globiformis cells showed no response. Approximately 50% of stressed Eb. aerogenes cells responded to valinomycin. The results demonstrate the limitations of rhodamine dye for viability analysing the viability of diverse bacterial communities and underline the degree of cell heterogeneity in batch cultures.

AB - The ionophore, valinomycin, was investigated as a possible means of bacterial viability assessment using the fluorescent membrane potential dye rhodamine 123. Membrane hyperpolarisation in Escherichia coli, Pseudomonas fluorescent, Enterobacter aerogenes and Arthrobacter globiformis was examined during exponential growth and during stress by brief starvation in a high sodium, low potassium buffer using flow cytometric analysis of rhodamine 123 uptake. Dye uptake was variable both between species and amongst cells from the same culture. Exponential phase cells showed no increase in dye uptake due to valinomycin treatment. Stressed P. fluorescens cells responded to valinomycin treatment by increased dye uptake, while stressed E. coli and A. globiformis cells showed no response. Approximately 50% of stressed Eb. aerogenes cells responded to valinomycin. The results demonstrate the limitations of rhodamine dye for viability analysing the viability of diverse bacterial communities and underline the degree of cell heterogeneity in batch cultures.

KW - Heterogeneity

KW - Membrane potential

KW - Stressed bacteria

KW - Valinomycin

U2 - 10.1016/0378-1097(95)00320-5

DO - 10.1016/0378-1097(95)00320-5

M3 - Journal article

C2 - 7590182

AN - SCOPUS:0028789384

VL - 132

SP - 259

EP - 262

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 3

ER -

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