Home > Research > Publications & Outputs > MERTK is a host factor that promotes classical ...

Links

Text available via DOI:

View graph of relations

MERTK is a host factor that promotes classical swine fever virus entry and antagonizes innate immune response in PK-15 cells

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

MERTK is a host factor that promotes classical swine fever virus entry and antagonizes innate immune response in PK-15 cells. / Zheng, G.; Li, L.-F.; Zhang, Y. et al.
In: Emerging Microbes and Infections, Vol. 9, No. 1, 01.05.2020, p. 571-581.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Zheng, G, Li, L-F, Zhang, Y, Qu, L, Wang, W, Li, M, Yu, S, Zhou, M, Luo, Y, Sun, Y, Munir, M, Li, S & Qiu, H-J 2020, 'MERTK is a host factor that promotes classical swine fever virus entry and antagonizes innate immune response in PK-15 cells', Emerging Microbes and Infections, vol. 9, no. 1, pp. 571-581. https://doi.org/10.1080/22221751.2020.1738278

APA

Zheng, G., Li, L-F., Zhang, Y., Qu, L., Wang, W., Li, M., Yu, S., Zhou, M., Luo, Y., Sun, Y., Munir, M., Li, S., & Qiu, H-J. (2020). MERTK is a host factor that promotes classical swine fever virus entry and antagonizes innate immune response in PK-15 cells. Emerging Microbes and Infections, 9(1), 571-581. https://doi.org/10.1080/22221751.2020.1738278

Vancouver

Zheng G, Li L-F, Zhang Y, Qu L, Wang W, Li M et al. MERTK is a host factor that promotes classical swine fever virus entry and antagonizes innate immune response in PK-15 cells. Emerging Microbes and Infections. 2020 May 1;9(1):571-581. Epub 2020 Mar 14. doi: 10.1080/22221751.2020.1738278

Author

Zheng, G. ; Li, L.-F. ; Zhang, Y. et al. / MERTK is a host factor that promotes classical swine fever virus entry and antagonizes innate immune response in PK-15 cells. In: Emerging Microbes and Infections. 2020 ; Vol. 9, No. 1. pp. 571-581.

Bibtex

@article{b998018dc34e45abaa77e6e26315ae2c,
title = "MERTK is a host factor that promotes classical swine fever virus entry and antagonizes innate immune response in PK-15 cells",
abstract = "Classical swine fever virus (CSFV) is a member of the genus Pestivirus in the Flaviviridae family. To date, the host factors required for CSFV entry remain poorly characterized. To identify the functional membrane protein(s) involved in CSFV infection, we analyzed the transcriptomic data from previous studies describing gene expression profiles for CSFV, and found twelve novel candidate proteins. One of these proteins, MERTK, significantly reduced CSFV protein expression by RNA interference screening using a recombinant CSFV that contains a luciferase reporter to measure CSFV protein expression. Furthermore, our results demonstrated that either anti-MERTK antibodies or soluble MERTK ectodomain could reduce CSFV infection in PK-15 cells in a dose-dependent manner. Mechanistically, MERTK interacted with the E2 protein of CSFV and facilitated virus entry. After virus entry, MERTK downregulates of mRNA expression of IFN-β and promotes CSFV infection. Interestingly, the soluble MERTK ectodomain could also reduce the infection of bovine viral diarrhea virus (BVDV), another pestivirus. Taken together, our results suggested that MERTK is a CSFV entry factor that synergistically dampens innate immune responses in PK-15 cells and is also involved in BVDV infection. ",
keywords = "classical swine fever virus, entry, innate immune response, MERTK, TAM receptors",
author = "G. Zheng and L.-F. Li and Y. Zhang and L. Qu and W. Wang and M. Li and S. Yu and M. Zhou and Y. Luo and Y. Sun and M. Munir and S. Li and H.-J. Qiu",
year = "2020",
month = may,
day = "1",
doi = "10.1080/22221751.2020.1738278",
language = "English",
volume = "9",
pages = "571--581",
journal = "Emerging Microbes and Infections",
number = "1",

}

RIS

TY - JOUR

T1 - MERTK is a host factor that promotes classical swine fever virus entry and antagonizes innate immune response in PK-15 cells

AU - Zheng, G.

AU - Li, L.-F.

AU - Zhang, Y.

AU - Qu, L.

AU - Wang, W.

AU - Li, M.

AU - Yu, S.

AU - Zhou, M.

AU - Luo, Y.

AU - Sun, Y.

AU - Munir, M.

AU - Li, S.

AU - Qiu, H.-J.

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Classical swine fever virus (CSFV) is a member of the genus Pestivirus in the Flaviviridae family. To date, the host factors required for CSFV entry remain poorly characterized. To identify the functional membrane protein(s) involved in CSFV infection, we analyzed the transcriptomic data from previous studies describing gene expression profiles for CSFV, and found twelve novel candidate proteins. One of these proteins, MERTK, significantly reduced CSFV protein expression by RNA interference screening using a recombinant CSFV that contains a luciferase reporter to measure CSFV protein expression. Furthermore, our results demonstrated that either anti-MERTK antibodies or soluble MERTK ectodomain could reduce CSFV infection in PK-15 cells in a dose-dependent manner. Mechanistically, MERTK interacted with the E2 protein of CSFV and facilitated virus entry. After virus entry, MERTK downregulates of mRNA expression of IFN-β and promotes CSFV infection. Interestingly, the soluble MERTK ectodomain could also reduce the infection of bovine viral diarrhea virus (BVDV), another pestivirus. Taken together, our results suggested that MERTK is a CSFV entry factor that synergistically dampens innate immune responses in PK-15 cells and is also involved in BVDV infection.

AB - Classical swine fever virus (CSFV) is a member of the genus Pestivirus in the Flaviviridae family. To date, the host factors required for CSFV entry remain poorly characterized. To identify the functional membrane protein(s) involved in CSFV infection, we analyzed the transcriptomic data from previous studies describing gene expression profiles for CSFV, and found twelve novel candidate proteins. One of these proteins, MERTK, significantly reduced CSFV protein expression by RNA interference screening using a recombinant CSFV that contains a luciferase reporter to measure CSFV protein expression. Furthermore, our results demonstrated that either anti-MERTK antibodies or soluble MERTK ectodomain could reduce CSFV infection in PK-15 cells in a dose-dependent manner. Mechanistically, MERTK interacted with the E2 protein of CSFV and facilitated virus entry. After virus entry, MERTK downregulates of mRNA expression of IFN-β and promotes CSFV infection. Interestingly, the soluble MERTK ectodomain could also reduce the infection of bovine viral diarrhea virus (BVDV), another pestivirus. Taken together, our results suggested that MERTK is a CSFV entry factor that synergistically dampens innate immune responses in PK-15 cells and is also involved in BVDV infection.

KW - classical swine fever virus

KW - entry

KW - innate immune response

KW - MERTK

KW - TAM receptors

U2 - 10.1080/22221751.2020.1738278

DO - 10.1080/22221751.2020.1738278

M3 - Journal article

C2 - 32172658

VL - 9

SP - 571

EP - 581

JO - Emerging Microbes and Infections

JF - Emerging Microbes and Infections

IS - 1

ER -