Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Molecular characterization of infectious bursal disease viruses from Pakistan
AU - Shabbir, Muhammad Zubair
AU - Ali, Muhammad
AU - Abbas, Muhammad
AU - Chaudhry, Umer Naveed
AU - Zia-Ur-Rehman, null
AU - Munir, Muhammad
PY - 2016/7
Y1 - 2016/7
N2 - Since the first report of infectious bursal disease in Pakistan in 1987, outbreaks have been common even in vaccinated flocks. Despite appropriate administration of vaccines, concerns arise if the circulating strains are different from the ones used in the vaccine. Here, we sequenced the hypervariable region (HVR) of the VP2 gene of circulating strains of infectious bursal disease virus (IBDV) originating from outbreaks (n = 4) in broiler flocks in Pakistan. Nucleotide sequencing followed by phylogeny and deduced amino acid sequence analysis showed the circulating strains to be very virulent (vv) and identified characteristic residues at position 222 (A), 242 (I), 256 (I), 294 (I) and 299 (S). In addition, a substitution at positions 221 (Q→H) was found to be exclusive to Pakistani strains in our analysis, although a larger dataset is required to confirm this finding. Compared to vaccine strains that are commonly used in Pakistan, substitution mutations were found at key amino acid positions in VP2 that may be responsible for potential changes in neutralization epitopes and vaccine failure.
AB - Since the first report of infectious bursal disease in Pakistan in 1987, outbreaks have been common even in vaccinated flocks. Despite appropriate administration of vaccines, concerns arise if the circulating strains are different from the ones used in the vaccine. Here, we sequenced the hypervariable region (HVR) of the VP2 gene of circulating strains of infectious bursal disease virus (IBDV) originating from outbreaks (n = 4) in broiler flocks in Pakistan. Nucleotide sequencing followed by phylogeny and deduced amino acid sequence analysis showed the circulating strains to be very virulent (vv) and identified characteristic residues at position 222 (A), 242 (I), 256 (I), 294 (I) and 299 (S). In addition, a substitution at positions 221 (Q→H) was found to be exclusive to Pakistani strains in our analysis, although a larger dataset is required to confirm this finding. Compared to vaccine strains that are commonly used in Pakistan, substitution mutations were found at key amino acid positions in VP2 that may be responsible for potential changes in neutralization epitopes and vaccine failure.
KW - Amino Acid Sequence
KW - Animals
KW - Birnaviridae Infections
KW - Capsid Proteins
KW - Chickens
KW - Disease Outbreaks
KW - Infectious bursal disease virus
KW - Molecular Sequence Data
KW - Pakistan
KW - Phylogeny
KW - Poultry Diseases
KW - Sequence Alignment
KW - Journal Article
U2 - 10.1007/s00705-016-2869-9
DO - 10.1007/s00705-016-2869-9
M3 - Journal article
C2 - 27107876
VL - 161
SP - 2001
EP - 2006
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 7
ER -