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Molecular characterization of newcastle disease virus genotype VIId in Avian influenza H5N1 infected broiler flock in Egypt

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Molecular characterization of newcastle disease virus genotype VIId in Avian influenza H5N1 infected broiler flock in Egypt. / Hussein, H. A.; Emara, M. M.; Rohaim, M. A.
In: International Journal of Virology, Vol. 10, No. 1, 2014, p. 46-54.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Hussein, HA, Emara, MM & Rohaim, MA 2014, 'Molecular characterization of newcastle disease virus genotype VIId in Avian influenza H5N1 infected broiler flock in Egypt', International Journal of Virology, vol. 10, no. 1, pp. 46-54. https://doi.org/10.3923/ijv.2014.46.54

APA

Hussein, H. A., Emara, M. M., & Rohaim, M. A. (2014). Molecular characterization of newcastle disease virus genotype VIId in Avian influenza H5N1 infected broiler flock in Egypt. International Journal of Virology, 10(1), 46-54. https://doi.org/10.3923/ijv.2014.46.54

Vancouver

Hussein HA, Emara MM, Rohaim MA. Molecular characterization of newcastle disease virus genotype VIId in Avian influenza H5N1 infected broiler flock in Egypt. International Journal of Virology. 2014;10(1):46-54. doi: 10.3923/ijv.2014.46.54

Author

Hussein, H. A. ; Emara, M. M. ; Rohaim, M. A. / Molecular characterization of newcastle disease virus genotype VIId in Avian influenza H5N1 infected broiler flock in Egypt. In: International Journal of Virology. 2014 ; Vol. 10, No. 1. pp. 46-54.

Bibtex

@article{665becf4afad4c639668297eddb11c1d,
title = "Molecular characterization of newcastle disease virus genotype VIId in Avian influenza H5N1 infected broiler flock in Egypt",
abstract = "In the early months of 2011, several devastating Newcastle disease outbreaks occurred in Egypt affecting commercial poultry. Both vaccinated and unvaccinated flocks were affected. In the present study, we characterized field isolate of NDV from a broiler flock coinfected with Avian influenza H5N1 showing characteristic clinical signs, post mortem gross lesions and was positive to A.I H5N1 and NDV by RT-PCR. The fusion protein gene of the Newcastle Disease Virus (NDV) isolate was partially amplified by RT-PCR, directly sequenced. The obtained sequence was submitted to GeneBank with the accession No. JX885868 (NDV/chicken/VRLCU138/Egypt/2012). Sequence was aligned and compared with a representative NDV isolates of different genotypes using NCBI BLAST. The F protein cleavage site sequence is a well-characterized determinant of NDV pathogenicity in chickens. The NDV isolate was found to have the motif 112RRQKRF117 which is indicative of the velogenic nature of this NDV isolate. Phylogenetic analysis revealed that then isolated NDV is belonging to subgenotype VIId and in close range to other Chinese VIId NDV isolates based on the high nucleotide similarity between them which indicate that genotype VIId is circulating between chicken flocks. This study reports the characterization of Newcastle Disease Virus genotype VIId in broiler chickens coinfected with Avian influenza H5N1 virus.",
keywords = "Avian influenza virus, Infected broiler flaks vaccine, Newcastle disease, Poultry",
author = "Hussein, {H. A.} and Emara, {M. M.} and Rohaim, {M. A.}",
year = "2014",
doi = "10.3923/ijv.2014.46.54",
language = "English",
volume = "10",
pages = "46--54",
journal = "International Journal of Virology",
issn = "1816-4900",
publisher = "Asian Network for Scientific Information",
number = "1",

}

RIS

TY - JOUR

T1 - Molecular characterization of newcastle disease virus genotype VIId in Avian influenza H5N1 infected broiler flock in Egypt

AU - Hussein, H. A.

AU - Emara, M. M.

AU - Rohaim, M. A.

PY - 2014

Y1 - 2014

N2 - In the early months of 2011, several devastating Newcastle disease outbreaks occurred in Egypt affecting commercial poultry. Both vaccinated and unvaccinated flocks were affected. In the present study, we characterized field isolate of NDV from a broiler flock coinfected with Avian influenza H5N1 showing characteristic clinical signs, post mortem gross lesions and was positive to A.I H5N1 and NDV by RT-PCR. The fusion protein gene of the Newcastle Disease Virus (NDV) isolate was partially amplified by RT-PCR, directly sequenced. The obtained sequence was submitted to GeneBank with the accession No. JX885868 (NDV/chicken/VRLCU138/Egypt/2012). Sequence was aligned and compared with a representative NDV isolates of different genotypes using NCBI BLAST. The F protein cleavage site sequence is a well-characterized determinant of NDV pathogenicity in chickens. The NDV isolate was found to have the motif 112RRQKRF117 which is indicative of the velogenic nature of this NDV isolate. Phylogenetic analysis revealed that then isolated NDV is belonging to subgenotype VIId and in close range to other Chinese VIId NDV isolates based on the high nucleotide similarity between them which indicate that genotype VIId is circulating between chicken flocks. This study reports the characterization of Newcastle Disease Virus genotype VIId in broiler chickens coinfected with Avian influenza H5N1 virus.

AB - In the early months of 2011, several devastating Newcastle disease outbreaks occurred in Egypt affecting commercial poultry. Both vaccinated and unvaccinated flocks were affected. In the present study, we characterized field isolate of NDV from a broiler flock coinfected with Avian influenza H5N1 showing characteristic clinical signs, post mortem gross lesions and was positive to A.I H5N1 and NDV by RT-PCR. The fusion protein gene of the Newcastle Disease Virus (NDV) isolate was partially amplified by RT-PCR, directly sequenced. The obtained sequence was submitted to GeneBank with the accession No. JX885868 (NDV/chicken/VRLCU138/Egypt/2012). Sequence was aligned and compared with a representative NDV isolates of different genotypes using NCBI BLAST. The F protein cleavage site sequence is a well-characterized determinant of NDV pathogenicity in chickens. The NDV isolate was found to have the motif 112RRQKRF117 which is indicative of the velogenic nature of this NDV isolate. Phylogenetic analysis revealed that then isolated NDV is belonging to subgenotype VIId and in close range to other Chinese VIId NDV isolates based on the high nucleotide similarity between them which indicate that genotype VIId is circulating between chicken flocks. This study reports the characterization of Newcastle Disease Virus genotype VIId in broiler chickens coinfected with Avian influenza H5N1 virus.

KW - Avian influenza virus

KW - Infected broiler flaks vaccine

KW - Newcastle disease

KW - Poultry

U2 - 10.3923/ijv.2014.46.54

DO - 10.3923/ijv.2014.46.54

M3 - Journal article

AN - SCOPUS:84898471094

VL - 10

SP - 46

EP - 54

JO - International Journal of Virology

JF - International Journal of Virology

SN - 1816-4900

IS - 1

ER -