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Molecular methods of virus detection in lymphoma

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Publication date2013
Host publicationLymphoma: methods and protocols
EditorsRalf Küppers
PublisherHumana Press
Pages277-293
Number of pages17
ISBN (Electronic)978-1-62703-269-8
ISBN (Print)978-1-62703-268-1
<mark>Original language</mark>English

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume971
ISSN (Print)1064-3745

Abstract

The herpesviruses Epstein-Barr virus (EBV) and human herpesvirus 8 and the retrovirus human T-cell leukemia virus type 1 are directly implicated in the pathogenesis of lymphoma and leukemia in man. EBV is associated with an expanding spectrum of lymphomas and it would appear likely that additional, possibly novel, viruses will be implicated in lymphoma pathogenesis in the future. This chapter describes techniques that may be useful in the analysis of viruses and lymphoma including a standard EBV EBER in situ hybridization assay and a degenerate PCR assay for detection of novel herpesviruses. Lastly, a method for analysis of next-generation sequences in the quest for novel viruses is described.