Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter
Molecular methods of virus detection in lymphoma. / Jarrett, Ruth F.; Gallagher, Alice; Gatherer, Derek.
Lymphoma: methods and protocols. ed. / Ralf Küppers. Humana Press, 2013. p. 277-293 (Methods in Molecular Biology; Vol. 971).Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter
}
TY - CHAP
T1 - Molecular methods of virus detection in lymphoma
AU - Jarrett, Ruth F.
AU - Gallagher, Alice
AU - Gatherer, Derek
PY - 2013
Y1 - 2013
N2 - The herpesviruses Epstein-Barr virus (EBV) and human herpesvirus 8 and the retrovirus human T-cell leukemia virus type 1 are directly implicated in the pathogenesis of lymphoma and leukemia in man. EBV is associated with an expanding spectrum of lymphomas and it would appear likely that additional, possibly novel, viruses will be implicated in lymphoma pathogenesis in the future. This chapter describes techniques that may be useful in the analysis of viruses and lymphoma including a standard EBV EBER in situ hybridization assay and a degenerate PCR assay for detection of novel herpesviruses. Lastly, a method for analysis of next-generation sequences in the quest for novel viruses is described.
AB - The herpesviruses Epstein-Barr virus (EBV) and human herpesvirus 8 and the retrovirus human T-cell leukemia virus type 1 are directly implicated in the pathogenesis of lymphoma and leukemia in man. EBV is associated with an expanding spectrum of lymphomas and it would appear likely that additional, possibly novel, viruses will be implicated in lymphoma pathogenesis in the future. This chapter describes techniques that may be useful in the analysis of viruses and lymphoma including a standard EBV EBER in situ hybridization assay and a degenerate PCR assay for detection of novel herpesviruses. Lastly, a method for analysis of next-generation sequences in the quest for novel viruses is described.
KW - DNA, Viral
KW - Gene Expression Profiling
KW - Herpesvirus 4, Human
KW - High-Throughput Nucleotide Sequencing
KW - Humans
KW - In Situ Hybridization
KW - Lymphoma
KW - Polymerase Chain Reaction
KW - Sequence Analysis, DNA
U2 - 10.1007/978-1-62703-269-8_16
DO - 10.1007/978-1-62703-269-8_16
M3 - Chapter
C2 - 23296970
SN - 978-1-62703-268-1
T3 - Methods in Molecular Biology
SP - 277
EP - 293
BT - Lymphoma
A2 - Küppers, Ralf
PB - Humana Press
ER -