NDV entry into dendritic cells through macropinocytosis and suppression of T lymphocyte proliferation

Biomedical and Life Sciences

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NDV entry into dendritic cells through macropinocytosis and suppression of T lymphocyte proliferation. / Tan, Lei; Zhang, Yuqiang; Qiao, Changtao et al.
In: Virology, Vol. 518, 05.2018, p. 126-135.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Tan, L, Zhang, Y, Qiao, C, Yuan, Y, Sun, Y, Qiu, X, Meng, C, Song, C, Liao, Y, Munir, M, Nair, V, Ding, Z, Liu, X & Ding, C 2018, 'NDV entry into dendritic cells through macropinocytosis and suppression of T lymphocyte proliferation', Virology, vol. 518, pp. 126-135. https://doi.org/10.1016/j.virol.2018.02.011

APA

Tan, L., Zhang, Y., Qiao, C., Yuan, Y., Sun, Y., Qiu, X., Meng, C., Song, C., Liao, Y., Munir, M., Nair, V., Ding, Z., Liu, X., & Ding, C. (2018). NDV entry into dendritic cells through macropinocytosis and suppression of T lymphocyte proliferation. Virology, 518, 126-135. https://doi.org/10.1016/j.virol.2018.02.011

Vancouver

Tan L, Zhang Y, Qiao C, Yuan Y, Sun Y, Qiu X et al. NDV entry into dendritic cells through macropinocytosis and suppression of T lymphocyte proliferation. Virology. 2018 May;518:126-135. Epub 2018 Feb 23. doi: 10.1016/j.virol.2018.02.011

Author

Tan, Lei ; Zhang, Yuqiang ; Qiao, Changtao et al. / NDV entry into dendritic cells through macropinocytosis and suppression of T lymphocyte proliferation. In: Virology. 2018 ; Vol. 518. pp. 126-135.

Bibtex

@article{fcde9ad15eb74a67a61966660bc1b0c6,

title = "NDV entry into dendritic cells through macropinocytosis and suppression of T lymphocyte proliferation",

abstract = "Newcastle disease virus (NDV) causes major economic losses in the poultry industry. Previous studies have shown that NDV utilizes different pathways to infect various cells, including dendritic cells (DCs). Here, we demonstrate that NDV gains entry into DCs mainly via macropinocytosis and clathrin-mediated endocytosis. The detection of cytokines interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-12 (IL-12), interleukin-4 (IL-4) and interleukin-10 (IL-10) indicates that NDV significantly induces Th1 responses and lowers Th2 responses. Furthermore, NDV entry into DCs resulted in the upregulation of TNF-related apoptosis-inducing ligand (TRAIL) and cleaved caspase-3 proteins, which in turn activated the extrinsic apoptosis pathway and induced DCs apoptosis. Transwell{\textregistered} co-culture demonstrated that direct contact between live NDV-stimulated DCs and T cells, rather than heated-inactivated NDV, inhibited CD4+T cell proliferation. Taken together, these findings provide new insights into the mechanism underlying NDV infections, particularly in relation to antigen presentation cells and suppression of T cell proliferation.",

keywords = "Newcastle disease virus, Dendritic cells, Macropinocytosis, Apoptosis, T lymphocyte proliferation",

author = "Lei Tan and Yuqiang Zhang and Changtao Qiao and Yanmei Yuan and Yingjie Sun and Xusheng Qiu and Chunchun Meng and Cuiping Song and Ying Liao and Muhammad Munir and Venugopal Nair and Zhuang Ding and Xiufan Liu and Chan Ding",

year = "2018",

month = may,

doi = "10.1016/j.virol.2018.02.011",

language = "English",

volume = "518",

pages = "126--135",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - NDV entry into dendritic cells through macropinocytosis and suppression of T lymphocyte proliferation

AU - Tan, Lei

AU - Zhang, Yuqiang

AU - Qiao, Changtao

AU - Yuan, Yanmei

AU - Sun, Yingjie

AU - Qiu, Xusheng

AU - Meng, Chunchun

AU - Song, Cuiping

AU - Liao, Ying

AU - Munir, Muhammad

AU - Nair, Venugopal

AU - Ding, Zhuang

AU - Liu, Xiufan

AU - Ding, Chan

PY - 2018/5

Y1 - 2018/5

N2 - Newcastle disease virus (NDV) causes major economic losses in the poultry industry. Previous studies have shown that NDV utilizes different pathways to infect various cells, including dendritic cells (DCs). Here, we demonstrate that NDV gains entry into DCs mainly via macropinocytosis and clathrin-mediated endocytosis. The detection of cytokines interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-12 (IL-12), interleukin-4 (IL-4) and interleukin-10 (IL-10) indicates that NDV significantly induces Th1 responses and lowers Th2 responses. Furthermore, NDV entry into DCs resulted in the upregulation of TNF-related apoptosis-inducing ligand (TRAIL) and cleaved caspase-3 proteins, which in turn activated the extrinsic apoptosis pathway and induced DCs apoptosis. Transwell® co-culture demonstrated that direct contact between live NDV-stimulated DCs and T cells, rather than heated-inactivated NDV, inhibited CD4+T cell proliferation. Taken together, these findings provide new insights into the mechanism underlying NDV infections, particularly in relation to antigen presentation cells and suppression of T cell proliferation.

AB - Newcastle disease virus (NDV) causes major economic losses in the poultry industry. Previous studies have shown that NDV utilizes different pathways to infect various cells, including dendritic cells (DCs). Here, we demonstrate that NDV gains entry into DCs mainly via macropinocytosis and clathrin-mediated endocytosis. The detection of cytokines interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-12 (IL-12), interleukin-4 (IL-4) and interleukin-10 (IL-10) indicates that NDV significantly induces Th1 responses and lowers Th2 responses. Furthermore, NDV entry into DCs resulted in the upregulation of TNF-related apoptosis-inducing ligand (TRAIL) and cleaved caspase-3 proteins, which in turn activated the extrinsic apoptosis pathway and induced DCs apoptosis. Transwell® co-culture demonstrated that direct contact between live NDV-stimulated DCs and T cells, rather than heated-inactivated NDV, inhibited CD4+T cell proliferation. Taken together, these findings provide new insights into the mechanism underlying NDV infections, particularly in relation to antigen presentation cells and suppression of T cell proliferation.

KW - Newcastle disease virus

KW - Dendritic cells

KW - Macropinocytosis

KW - Apoptosis

KW - T lymphocyte proliferation

U2 - 10.1016/j.virol.2018.02.011

DO - 10.1016/j.virol.2018.02.011

M3 - Journal article

C2 - 29481983

VL - 518

SP - 126

EP - 135

JO - Virology

JF - Virology

SN - 0042-6822

ER -

Research

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