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Neutrophils mediate the capture of peritoneal contaminants by fat-associated lymphoid clusters of the omentum

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Neutrophils mediate the capture of peritoneal contaminants by fat-associated lymphoid clusters of the omentum. / Jackson-Jones, Lucy; Smith, Peter; Magalhaes, Marlene et al.
In: Biorxiv, 19.09.2019.

Research output: Contribution to Journal/Magazine › Journal article

Harvard

Jackson-Jones, L, Smith, P, Magalhaes, M, Portman, J, Mylonas, K, Nixon, M, Dobie, R, Henderson, B, Henderson, N, Mole, D & Benezech, C 2019, 'Neutrophils mediate the capture of peritoneal contaminants by fat-associated lymphoid clusters of the omentum', Biorxiv. https://doi.org/10.1101/774968

APA

Jackson-Jones, L., Smith, P., Magalhaes, M., Portman, J., Mylonas, K., Nixon, M., Dobie, R., Henderson, B., Henderson, N., Mole, D., & Benezech, C. (2019). Neutrophils mediate the capture of peritoneal contaminants by fat-associated lymphoid clusters of the omentum. Biorxiv. Advance online publication. https://doi.org/10.1101/774968

Vancouver

Jackson-Jones L, Smith P, Magalhaes M, Portman J, Mylonas K, Nixon M et al. Neutrophils mediate the capture of peritoneal contaminants by fat-associated lymphoid clusters of the omentum. Biorxiv. 2019 Sept 19. Epub 2019 Sept 19. doi: 10.1101/774968

Author

Jackson-Jones, Lucy ; Smith, Peter ; Magalhaes, Marlene et al. / Neutrophils mediate the capture of peritoneal contaminants by fat-associated lymphoid clusters of the omentum. In: Biorxiv. 2019.

Bibtex

@article{7fe20aaa01a64f64936ee63690334ec6,
title = "Neutrophils mediate the capture of peritoneal contaminants by fat-associated lymphoid clusters of the omentum",
abstract = "The omentum is a visceral adipose tissue rich in fat-associated lymphoid clusters (FALCs), which collects peritoneal contaminants and provides a first layer of immunological defence within the abdomen. Using single-cell RNA sequencing and spatial analysis of omental stromal cells, we reveal that the surface of FALCs are covered with specialised mesothelial cells, which we name FALC cover cells. We demonstrate that CXCL1 is expressed by FALC cover cells and that CXCL1 is critical for the retention and accumulation of neutrophils within FALCs during peritonitis. We show that protein arginine deiminase 4 mediates the formation of dense neutrophil aggregates, which are required for the neutralisation of particles present in the peritoneal cavity. Finally, we provide confirmatory evidence in humans with acute appendicitis, that the omentum is also a site of neutrophil recruitment and bacterial capture, and is thus an important component of the immunological defence against the propagation of peritoneal contaminants.",
author = "Lucy Jackson-Jones and Peter Smith and Marlene Magalhaes and Jordan Portman and Katie Mylonas and Mark Nixon and Ross Dobie and Beth Henderson and Neil Henderson and Damian Mole and Cecile Benezech",
year = "2019",
month = sep,
day = "19",
doi = "10.1101/774968",
language = "English",
journal = "Biorxiv",
publisher = "Cold Spring Harbor Laboratory Press",

}

RIS

TY - JOUR

T1 - Neutrophils mediate the capture of peritoneal contaminants by fat-associated lymphoid clusters of the omentum

AU - Jackson-Jones, Lucy

AU - Smith, Peter

AU - Magalhaes, Marlene

AU - Portman, Jordan

AU - Mylonas, Katie

AU - Nixon, Mark

AU - Dobie, Ross

AU - Henderson, Beth

AU - Henderson, Neil

AU - Mole, Damian

AU - Benezech, Cecile

PY - 2019/9/19

Y1 - 2019/9/19

N2 - The omentum is a visceral adipose tissue rich in fat-associated lymphoid clusters (FALCs), which collects peritoneal contaminants and provides a first layer of immunological defence within the abdomen. Using single-cell RNA sequencing and spatial analysis of omental stromal cells, we reveal that the surface of FALCs are covered with specialised mesothelial cells, which we name FALC cover cells. We demonstrate that CXCL1 is expressed by FALC cover cells and that CXCL1 is critical for the retention and accumulation of neutrophils within FALCs during peritonitis. We show that protein arginine deiminase 4 mediates the formation of dense neutrophil aggregates, which are required for the neutralisation of particles present in the peritoneal cavity. Finally, we provide confirmatory evidence in humans with acute appendicitis, that the omentum is also a site of neutrophil recruitment and bacterial capture, and is thus an important component of the immunological defence against the propagation of peritoneal contaminants.

AB - The omentum is a visceral adipose tissue rich in fat-associated lymphoid clusters (FALCs), which collects peritoneal contaminants and provides a first layer of immunological defence within the abdomen. Using single-cell RNA sequencing and spatial analysis of omental stromal cells, we reveal that the surface of FALCs are covered with specialised mesothelial cells, which we name FALC cover cells. We demonstrate that CXCL1 is expressed by FALC cover cells and that CXCL1 is critical for the retention and accumulation of neutrophils within FALCs during peritonitis. We show that protein arginine deiminase 4 mediates the formation of dense neutrophil aggregates, which are required for the neutralisation of particles present in the peritoneal cavity. Finally, we provide confirmatory evidence in humans with acute appendicitis, that the omentum is also a site of neutrophil recruitment and bacterial capture, and is thus an important component of the immunological defence against the propagation of peritoneal contaminants.

U2 - 10.1101/774968

DO - 10.1101/774968

M3 - Journal article

JO - Biorxiv

JF - Biorxiv

ER -