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    Rights statement: This is the peer reviewed version of the following article: Santos, MR, Xavier, PLP, Pires, PRL, et al. Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines. Vet Comp Oncol. 2021; 19: 593– 601. doi.org/10.1111/vco.12699 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/vco.12699 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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    Embargo ends: 30/04/22

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Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines

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Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines. / Santos, Mariana Rodrigues; Xavier, Pedro Luiz Porfírio; Pires, Pedro Ratto Lisboa; Rochetti, Arina Lázaro; Rosim, Daniele Fernanda; Scagion, Guilherme Pereira; Zuccari, Debora Aparecida Pires Campos; Munir, Muhammad; Ferreira, Helena Lage; Fukumasu, Heidge.

In: Veterinary and Comparative Oncology, Vol. 19, No. 3, 30.09.2021, p. 593-601.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Santos, MR, Xavier, PLP, Pires, PRL, Rochetti, AL, Rosim, DF, Scagion, GP, Zuccari, DAPC, Munir, M, Ferreira, HL & Fukumasu, H 2021, 'Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines', Veterinary and Comparative Oncology, vol. 19, no. 3, pp. 593-601. https://doi.org/10.1111/vco.12699

APA

Santos, M. R., Xavier, P. L. P., Pires, P. R. L., Rochetti, A. L., Rosim, D. F., Scagion, G. P., Zuccari, D. A. P. C., Munir, M., Ferreira, H. L., & Fukumasu, H. (2021). Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines. Veterinary and Comparative Oncology, 19(3), 593-601. https://doi.org/10.1111/vco.12699

Vancouver

Santos MR, Xavier PLP, Pires PRL, Rochetti AL, Rosim DF, Scagion GP et al. Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines. Veterinary and Comparative Oncology. 2021 Sep 30;19(3):593-601. https://doi.org/10.1111/vco.12699

Author

Santos, Mariana Rodrigues ; Xavier, Pedro Luiz Porfírio ; Pires, Pedro Ratto Lisboa ; Rochetti, Arina Lázaro ; Rosim, Daniele Fernanda ; Scagion, Guilherme Pereira ; Zuccari, Debora Aparecida Pires Campos ; Munir, Muhammad ; Ferreira, Helena Lage ; Fukumasu, Heidge. / Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines. In: Veterinary and Comparative Oncology. 2021 ; Vol. 19, No. 3. pp. 593-601.

Bibtex

@article{3959bb02237f439791655f5fa179ad23,
title = "Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines",
abstract = "Canine mammary carcinoma (CMC) is one of the major health threats in dogs. The oncolytic virotherapy is a promising strategy to treat canine as well as human cancer patients with non‐pathogenic replicating viruses. Here, we evaluated the antitumor activity of one lentogenic, non‐lytic Newcastle disease virus (NDV) LaSota strain expressing GFP (NDV‐GFP) on five different CMCs and one non‐tumorigenic cell line, regarding cell viability, cell death, selectivity index, morphology, global and target gene expression analysis. As evidenced by the selectivity index, all CMC cell lines were more susceptible to NDV‐GFP in comparison with the non‐tumorigenic cells (~3.1× to ~78.7×). In addition, the oncolytic effect of NDV‐GFP was more evident in more malignant CMC cells. Also, we observed an inverse association of the IFN pathway expression and the susceptibility to NDV. The downregulated genes in NDV‐GFP‐sensitive cells were functionally enriched for antiviral mechanisms by interferon and immune system pathways, demonstrating that these mechanisms are the most prominent for oncolysis by NDV. To our knowledge, this is the first description of oncolysis by an NDV strain in canine mammary cancer cells. We also demonstrated specific molecular pathways related to NDV susceptibility in these cancer cells, opening the possibility to use NDV as a therapeutic‐targeted option for more malignant CMCs. Therefore, these results urge for more studies using oncolytic NDVs, especially considering genetic editing to improve efficacy in dogs.",
keywords = "Comparative oncology, IFN-β, Paramyxovirus, RNA-seq, Selectivity index",
author = "Santos, {Mariana Rodrigues} and Xavier, {Pedro Luiz Porf{\'i}rio} and Pires, {Pedro Ratto Lisboa} and Rochetti, {Arina L{\'a}zaro} and Rosim, {Daniele Fernanda} and Scagion, {Guilherme Pereira} and Zuccari, {Debora Aparecida Pires Campos} and Muhammad Munir and Ferreira, {Helena Lage} and Heidge Fukumasu",
note = "This is the peer reviewed version of the following article: Santos, MR, Xavier, PLP, Pires, PRL, et al. Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines. Vet Comp Oncol. 2021; 19: 593– 601. doi.org/10.1111/vco.12699 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/vco.12699 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving. ",
year = "2021",
month = sep,
day = "30",
doi = "10.1111/vco.12699",
language = "English",
volume = "19",
pages = "593--601",
journal = "Veterinary and Comparative Oncology",
number = "3",

}

RIS

TY - JOUR

T1 - Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines

AU - Santos, Mariana Rodrigues

AU - Xavier, Pedro Luiz Porfírio

AU - Pires, Pedro Ratto Lisboa

AU - Rochetti, Arina Lázaro

AU - Rosim, Daniele Fernanda

AU - Scagion, Guilherme Pereira

AU - Zuccari, Debora Aparecida Pires Campos

AU - Munir, Muhammad

AU - Ferreira, Helena Lage

AU - Fukumasu, Heidge

N1 - This is the peer reviewed version of the following article: Santos, MR, Xavier, PLP, Pires, PRL, et al. Oncolytic effect of Newcastle disease virus is attributed to interferon regulation in canine mammary cancer cell lines. Vet Comp Oncol. 2021; 19: 593– 601. doi.org/10.1111/vco.12699 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/vco.12699 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

PY - 2021/9/30

Y1 - 2021/9/30

N2 - Canine mammary carcinoma (CMC) is one of the major health threats in dogs. The oncolytic virotherapy is a promising strategy to treat canine as well as human cancer patients with non‐pathogenic replicating viruses. Here, we evaluated the antitumor activity of one lentogenic, non‐lytic Newcastle disease virus (NDV) LaSota strain expressing GFP (NDV‐GFP) on five different CMCs and one non‐tumorigenic cell line, regarding cell viability, cell death, selectivity index, morphology, global and target gene expression analysis. As evidenced by the selectivity index, all CMC cell lines were more susceptible to NDV‐GFP in comparison with the non‐tumorigenic cells (~3.1× to ~78.7×). In addition, the oncolytic effect of NDV‐GFP was more evident in more malignant CMC cells. Also, we observed an inverse association of the IFN pathway expression and the susceptibility to NDV. The downregulated genes in NDV‐GFP‐sensitive cells were functionally enriched for antiviral mechanisms by interferon and immune system pathways, demonstrating that these mechanisms are the most prominent for oncolysis by NDV. To our knowledge, this is the first description of oncolysis by an NDV strain in canine mammary cancer cells. We also demonstrated specific molecular pathways related to NDV susceptibility in these cancer cells, opening the possibility to use NDV as a therapeutic‐targeted option for more malignant CMCs. Therefore, these results urge for more studies using oncolytic NDVs, especially considering genetic editing to improve efficacy in dogs.

AB - Canine mammary carcinoma (CMC) is one of the major health threats in dogs. The oncolytic virotherapy is a promising strategy to treat canine as well as human cancer patients with non‐pathogenic replicating viruses. Here, we evaluated the antitumor activity of one lentogenic, non‐lytic Newcastle disease virus (NDV) LaSota strain expressing GFP (NDV‐GFP) on five different CMCs and one non‐tumorigenic cell line, regarding cell viability, cell death, selectivity index, morphology, global and target gene expression analysis. As evidenced by the selectivity index, all CMC cell lines were more susceptible to NDV‐GFP in comparison with the non‐tumorigenic cells (~3.1× to ~78.7×). In addition, the oncolytic effect of NDV‐GFP was more evident in more malignant CMC cells. Also, we observed an inverse association of the IFN pathway expression and the susceptibility to NDV. The downregulated genes in NDV‐GFP‐sensitive cells were functionally enriched for antiviral mechanisms by interferon and immune system pathways, demonstrating that these mechanisms are the most prominent for oncolysis by NDV. To our knowledge, this is the first description of oncolysis by an NDV strain in canine mammary cancer cells. We also demonstrated specific molecular pathways related to NDV susceptibility in these cancer cells, opening the possibility to use NDV as a therapeutic‐targeted option for more malignant CMCs. Therefore, these results urge for more studies using oncolytic NDVs, especially considering genetic editing to improve efficacy in dogs.

KW - Comparative oncology

KW - IFN-β

KW - Paramyxovirus

KW - RNA-seq

KW - Selectivity index

U2 - 10.1111/vco.12699

DO - 10.1111/vco.12699

M3 - Journal article

VL - 19

SP - 593

EP - 601

JO - Veterinary and Comparative Oncology

JF - Veterinary and Comparative Oncology

IS - 3

ER -