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Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network

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Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network. / Kamps, Dominic; Koch, Johannes; Juma, Victor O. et al.
In: Cell Reports, Vol. 33, No. 9, 108467, 01.12.2020.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Kamps, D, Koch, J, Juma, VO, Campillo-Funollet, E, Graessl, M, Banerjee, S, Mazel, T, Chen, X, Wu, YW, Portet, S, Madzvamuse, A, Nalbant, P & Dehmelt, L 2020, 'Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network', Cell Reports, vol. 33, no. 9, 108467. https://doi.org/10.1016/j.celrep.2020.108467

APA

Kamps, D., Koch, J., Juma, V. O., Campillo-Funollet, E., Graessl, M., Banerjee, S., Mazel, T., Chen, X., Wu, Y. W., Portet, S., Madzvamuse, A., Nalbant, P., & Dehmelt, L. (2020). Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network. Cell Reports, 33(9), Article 108467. https://doi.org/10.1016/j.celrep.2020.108467

Vancouver

Kamps D, Koch J, Juma VO, Campillo-Funollet E, Graessl M, Banerjee S et al. Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network. Cell Reports. 2020 Dec 1;33(9):108467. doi: 10.1016/j.celrep.2020.108467

Author

Kamps, Dominic ; Koch, Johannes ; Juma, Victor O. et al. / Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network. In: Cell Reports. 2020 ; Vol. 33, No. 9.

Bibtex

@article{0a392b02a2c341509c408662adfe70bb,
title = "Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network",
abstract = "Local cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system of ordinary differential equations describing temporal signal network dynamics. Bifurcation analysis and numerical simulations predict a strong dependence of oscillatory system dynamics on the concentration of GEF-H1, an Lbc-type RhoGEF, which mediates the positive feedback amplification of Rho activity. This prediction is confirmed experimentally via optogenetic tuning of the effective GEF-H1 concentration in individual living cells. Numerical simulations show that pulse amplitude is most sensitive to external inputs into the myosin component at low GEF-H1 concentrations and that the spatial pulse width is dependent on GEF-H1 diffusion. Our study offers a theoretical framework to explain the emergence of local cell contraction pulses and their modulation by biochemical and mechanical signals.",
keywords = "cell contraction, cytoskeleton, dynamical system, mechanotransduction, myosin, optogenetics, oscillations, parameter inference, reaction-diffusion system, rho GTPase",
author = "Dominic Kamps and Johannes Koch and Juma, {Victor O.} and Eduard Campillo-Funollet and Melanie Graessl and Soumya Banerjee and Tom{\'a}{\v s} Mazel and Xi Chen and Wu, {Yao Wen} and Stephanie Portet and Anotida Madzvamuse and Perihan Nalbant and Leif Dehmelt",
year = "2020",
month = dec,
day = "1",
doi = "10.1016/j.celrep.2020.108467",
language = "English",
volume = "33",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "9",

}

RIS

TY - JOUR

T1 - Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network

AU - Kamps, Dominic

AU - Koch, Johannes

AU - Juma, Victor O.

AU - Campillo-Funollet, Eduard

AU - Graessl, Melanie

AU - Banerjee, Soumya

AU - Mazel, Tomáš

AU - Chen, Xi

AU - Wu, Yao Wen

AU - Portet, Stephanie

AU - Madzvamuse, Anotida

AU - Nalbant, Perihan

AU - Dehmelt, Leif

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Local cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system of ordinary differential equations describing temporal signal network dynamics. Bifurcation analysis and numerical simulations predict a strong dependence of oscillatory system dynamics on the concentration of GEF-H1, an Lbc-type RhoGEF, which mediates the positive feedback amplification of Rho activity. This prediction is confirmed experimentally via optogenetic tuning of the effective GEF-H1 concentration in individual living cells. Numerical simulations show that pulse amplitude is most sensitive to external inputs into the myosin component at low GEF-H1 concentrations and that the spatial pulse width is dependent on GEF-H1 diffusion. Our study offers a theoretical framework to explain the emergence of local cell contraction pulses and their modulation by biochemical and mechanical signals.

AB - Local cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system of ordinary differential equations describing temporal signal network dynamics. Bifurcation analysis and numerical simulations predict a strong dependence of oscillatory system dynamics on the concentration of GEF-H1, an Lbc-type RhoGEF, which mediates the positive feedback amplification of Rho activity. This prediction is confirmed experimentally via optogenetic tuning of the effective GEF-H1 concentration in individual living cells. Numerical simulations show that pulse amplitude is most sensitive to external inputs into the myosin component at low GEF-H1 concentrations and that the spatial pulse width is dependent on GEF-H1 diffusion. Our study offers a theoretical framework to explain the emergence of local cell contraction pulses and their modulation by biochemical and mechanical signals.

KW - cell contraction

KW - cytoskeleton

KW - dynamical system

KW - mechanotransduction

KW - myosin

KW - optogenetics

KW - oscillations

KW - parameter inference

KW - reaction-diffusion system

KW - rho GTPase

U2 - 10.1016/j.celrep.2020.108467

DO - 10.1016/j.celrep.2020.108467

M3 - Journal article

C2 - 33264629

AN - SCOPUS:85097122341

VL - 33

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 9

M1 - 108467

ER -