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Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl.

Research output: Contribution to journalJournal article

Published
  • I. Bancroft
  • R. J. Smith
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<mark>Journal publication date</mark>1992
<mark>Journal</mark>New Phytologist
Issue number4
Volume121
Number of pages5
Pages (from-to)571-575
Publication StatusPublished
<mark>Original language</mark>English

Abstract

The low tolerance of cyanophage AN-23 to NaCl at more than 0·05 M has been investigated. When isolated from clarified lysates that had been exposed to NaCl in the presence of added DNAse I, the phage genomic DNA consisted of a series of discrete fragments. The fragments were recovered from low melting temperature agarose electrophoresis gels and used for restriction analysis. Comparison of the restriction cleavage products with a restriction map of the 46 kbp AN-23 genome has shown that the fragments are generated by removal of DNA from one end of the linear genome in steps of about 3 kbp. The data are consistent with a model in which NaCl triggers the ejection of the phage genome from the capsid.