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Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl.

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Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl. / Bancroft, I.; Smith, R. J.
In: New Phytologist, Vol. 121, No. 4, 1992, p. 571-575.

Research output: Contribution to Journal/Magazine › Journal article

Harvard

Bancroft, I & Smith, RJ 1992, 'Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl.', New Phytologist, vol. 121, no. 4, pp. 571-575. https://doi.org/10.1111/j.1469-8137.1992.tb01127.x

APA

Bancroft, I., & Smith, R. J. (1992). Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl. New Phytologist, 121(4), 571-575. https://doi.org/10.1111/j.1469-8137.1992.tb01127.x

Vancouver

Bancroft I, Smith RJ. Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl. New Phytologist. 1992;121(4):571-575. doi: 10.1111/j.1469-8137.1992.tb01127.x

Author

Bancroft, I. ; Smith, R. J. / Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl. In: New Phytologist. 1992 ; Vol. 121, No. 4. pp. 571-575.

Bibtex

@article{05e1ef46fb6f4308b67f1eff28691094,
title = "Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl.",
abstract = "The low tolerance of cyanophage AN-23 to NaCl at more than 0·05 M has been investigated. When isolated from clarified lysates that had been exposed to NaCl in the presence of added DNAse I, the phage genomic DNA consisted of a series of discrete fragments. The fragments were recovered from low melting temperature agarose electrophoresis gels and used for restriction analysis. Comparison of the restriction cleavage products with a restriction map of the 46 kbp AN-23 genome has shown that the fragments are generated by removal of DNA from one end of the linear genome in steps of about 3 kbp. The data are consistent with a model in which NaCl triggers the ejection of the phage genome from the capsid.",
keywords = "Cyanobacterial virus • cyanophage AN-23 • NaCl-promoted degradation",
author = "I. Bancroft and Smith, {R. J.}",
year = "1992",
doi = "10.1111/j.1469-8137.1992.tb01127.x",
language = "English",
volume = "121",
pages = "571--575",
journal = "New Phytologist",
publisher = "Wiley",
number = "4",

}

RIS

TY - JOUR

T1 - Oriented degradation of the genome of cyanophage AN-23 after exposure to NaCl.

AU - Bancroft, I.

AU - Smith, R. J.

PY - 1992

Y1 - 1992

N2 - The low tolerance of cyanophage AN-23 to NaCl at more than 0·05 M has been investigated. When isolated from clarified lysates that had been exposed to NaCl in the presence of added DNAse I, the phage genomic DNA consisted of a series of discrete fragments. The fragments were recovered from low melting temperature agarose electrophoresis gels and used for restriction analysis. Comparison of the restriction cleavage products with a restriction map of the 46 kbp AN-23 genome has shown that the fragments are generated by removal of DNA from one end of the linear genome in steps of about 3 kbp. The data are consistent with a model in which NaCl triggers the ejection of the phage genome from the capsid.

AB - The low tolerance of cyanophage AN-23 to NaCl at more than 0·05 M has been investigated. When isolated from clarified lysates that had been exposed to NaCl in the presence of added DNAse I, the phage genomic DNA consisted of a series of discrete fragments. The fragments were recovered from low melting temperature agarose electrophoresis gels and used for restriction analysis. Comparison of the restriction cleavage products with a restriction map of the 46 kbp AN-23 genome has shown that the fragments are generated by removal of DNA from one end of the linear genome in steps of about 3 kbp. The data are consistent with a model in which NaCl triggers the ejection of the phage genome from the capsid.

KW - Cyanobacterial virus • cyanophage AN-23 • NaCl-promoted degradation

U2 - 10.1111/j.1469-8137.1992.tb01127.x

DO - 10.1111/j.1469-8137.1992.tb01127.x

M3 - Journal article

VL - 121

SP - 571

EP - 575

JO - New Phytologist

JF - New Phytologist

IS - 4

ER -