Home > Research > Publications & Outputs > Oxidative Stress Pathways Linked to Apoptosis I...

Research

Associated organisational units

Links

Text available via DOI:

Keywords

View graph of relations

Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study. / Fukuhara, Hideo; Szili, Endre; Oh, Jun-Seok et al.
In: PLASMA, Vol. 5, No. 2, 27.04.2022, p. 233-246.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Fukuhara, H, Szili, E, Oh, J-S, Kawada, C, Yamamoto, S, Kurabayashi, A, Furihata, M, Tsuda, M, Furuta, H, Lindsay, H, Short, RD, Hatta, A & Inoue, K 2022, 'Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study', PLASMA, vol. 5, no. 2, pp. 233-246. https://doi.org/10.3390/plasma5020018

APA

Fukuhara, H., Szili, E., Oh, J.-S., Kawada, C., Yamamoto, S., Kurabayashi, A., Furihata, M., Tsuda, M., Furuta, H., Lindsay, H., Short, R. D., Hatta, A., & Inoue, K. (2022). Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study. PLASMA, 5(2), 233-246. https://doi.org/10.3390/plasma5020018

Vancouver

Fukuhara H, Szili E, Oh JS, Kawada C, Yamamoto S, Kurabayashi A et al. Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study. PLASMA. 2022 Apr 27;5(2):233-246. doi: 10.3390/plasma5020018

Author

Fukuhara, Hideo ; Szili, Endre ; Oh, Jun-Seok et al. / Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells : An In Vitro and In Vivo Study. In: PLASMA. 2022 ; Vol. 5, No. 2. pp. 233-246.

Bibtex

@article{73e529076d4a40f59f86a8254e391f33,
title = "Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study",
abstract = "Current methods used to treat non-muscle invasive bladder cancer are inadequate due to a high recurrence rate after surgery and the occurrence of adverse events such as interstitial pneumonia following intravesical instillation therapy. Low-temperature plasma is a new form of physical therapythat provides a rich source of reactive oxygen species (ROS). Oxidative solutions, created by pretreatment of aqueous media with plasma before application to target cells, lead to the destruction of cancer cells through oxidative stress pathways. This study focuses on the effects of plasma-activated media (PAM) in bladder cancer cells. PAM treatment increases oxidative stress that leads to cell cycle arrest and concomitantly depolarises the mitochondrial membrane leading to increased mitochondrial ROS production. Cell cycle arrest and increased mitochondrial ROS production led to an increase in caspase 3/cytochrome c activity, which might explain the induction of apoptosis in bladder cancer cells in vitro and in a bladder cancer tumour in vivo. These observations highlight the potential of plasma activated solutions as a new adjuvant therapy in the clinical treatment of bladder cancer.",
keywords = "plasma activated media, bladder cancer;, reactive oxygen species, oxidative stress, caspase 3, cytochrome c, cell cycle;, tumor, plasma jet;, apoptosis",
author = "Hideo Fukuhara and Endre Szili and Jun-Seok Oh and Chiaki Kawada and Shinkuro Yamamoto and Atsushi Kurabayashi and Mutsuo Furihata and Masayuki Tsuda and Hiroshi Furuta and Howard Lindsay and Short, {Robert D} and Akimitsu Hatta and Keiji Inoue",
year = "2022",
month = apr,
day = "27",
doi = "10.3390/plasma5020018",
language = "English",
volume = "5",
pages = "233--246",
journal = "PLASMA",
issn = "2571-6182",
publisher = "MDPI - Open Access Publishing",
number = "2",

}

RIS

TY - JOUR

T1 - Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells

T2 - An In Vitro and In Vivo Study

AU - Fukuhara, Hideo

AU - Szili, Endre

AU - Oh, Jun-Seok

AU - Kawada, Chiaki

AU - Yamamoto, Shinkuro

AU - Kurabayashi, Atsushi

AU - Furihata, Mutsuo

AU - Tsuda, Masayuki

AU - Furuta, Hiroshi

AU - Lindsay, Howard

AU - Short, Robert D

AU - Hatta, Akimitsu

AU - Inoue, Keiji

PY - 2022/4/27

Y1 - 2022/4/27

N2 - Current methods used to treat non-muscle invasive bladder cancer are inadequate due to a high recurrence rate after surgery and the occurrence of adverse events such as interstitial pneumonia following intravesical instillation therapy. Low-temperature plasma is a new form of physical therapythat provides a rich source of reactive oxygen species (ROS). Oxidative solutions, created by pretreatment of aqueous media with plasma before application to target cells, lead to the destruction of cancer cells through oxidative stress pathways. This study focuses on the effects of plasma-activated media (PAM) in bladder cancer cells. PAM treatment increases oxidative stress that leads to cell cycle arrest and concomitantly depolarises the mitochondrial membrane leading to increased mitochondrial ROS production. Cell cycle arrest and increased mitochondrial ROS production led to an increase in caspase 3/cytochrome c activity, which might explain the induction of apoptosis in bladder cancer cells in vitro and in a bladder cancer tumour in vivo. These observations highlight the potential of plasma activated solutions as a new adjuvant therapy in the clinical treatment of bladder cancer.

AB - Current methods used to treat non-muscle invasive bladder cancer are inadequate due to a high recurrence rate after surgery and the occurrence of adverse events such as interstitial pneumonia following intravesical instillation therapy. Low-temperature plasma is a new form of physical therapythat provides a rich source of reactive oxygen species (ROS). Oxidative solutions, created by pretreatment of aqueous media with plasma before application to target cells, lead to the destruction of cancer cells through oxidative stress pathways. This study focuses on the effects of plasma-activated media (PAM) in bladder cancer cells. PAM treatment increases oxidative stress that leads to cell cycle arrest and concomitantly depolarises the mitochondrial membrane leading to increased mitochondrial ROS production. Cell cycle arrest and increased mitochondrial ROS production led to an increase in caspase 3/cytochrome c activity, which might explain the induction of apoptosis in bladder cancer cells in vitro and in a bladder cancer tumour in vivo. These observations highlight the potential of plasma activated solutions as a new adjuvant therapy in the clinical treatment of bladder cancer.

KW - plasma activated media

KW - bladder cancer;

KW - reactive oxygen species

KW - oxidative stress

KW - caspase 3

KW - cytochrome c

KW - cell cycle;

KW - tumor

KW - plasma jet;

KW - apoptosis

U2 - 10.3390/plasma5020018

DO - 10.3390/plasma5020018

M3 - Journal article

VL - 5

SP - 233

EP - 246

JO - PLASMA

JF - PLASMA

SN - 2571-6182

IS - 2

ER -