Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Prednisolone reduces preconceptual endometrial natural killer cells in women with recurrent miscarriage.
AU - Quenby, Siobhan
AU - Kalumbi, Chimwemwe
AU - Bates, Michelle
AU - Farquharson,, Roy
AU - Vince, Gill S.
N1 - RAE_import_type : Journal article RAE_uoa_type : Allied Health Professions and Studies
PY - 2005/10/1
Y1 - 2005/10/1
N2 - Objective To test the hypothesis that high numbers of uterine natural killer (uNK) cells in the endometrium of women with recurrent miscarriage (RM) could be reduced with prednisolone. Design A before and after study. Setting A tertiary referral teaching hospital. Patient(s) Eighty-five women with idiopathic RM recruited from all over the UK and 18 women attending for sterilization (controls). Intervention(s) An endometrial sample was taken on day 21 ± 2 of the menstrual cycle. Immunohistochemistry was used to identify uNK (CD56+, CD16−, CD3−). Twenty-nine women with RM and >5% uNK agreed to take 20 mg oral prednisolone daily from day 1 to 21 of their menstrual cycle, when a second biopsy was obtained and analyzed. Main Outcome Measure(s) The percentage of stromal cells that were uNK. The normal range was defined using control samples as <5%. Result(s) Women with RM had significantly more uNK than the controls (P=.008). Prednisolone treatment significantly reduced the number of CD56 cells in the endometrium, from a median of 14% (before) to 9% (after) (P=.0004). Conclusion(s) We have demonstrated that high numbers of uNK in preimplantation endometrium of women with recurrent miscarriage can be reduced with administration of prednisolone.
AB - Objective To test the hypothesis that high numbers of uterine natural killer (uNK) cells in the endometrium of women with recurrent miscarriage (RM) could be reduced with prednisolone. Design A before and after study. Setting A tertiary referral teaching hospital. Patient(s) Eighty-five women with idiopathic RM recruited from all over the UK and 18 women attending for sterilization (controls). Intervention(s) An endometrial sample was taken on day 21 ± 2 of the menstrual cycle. Immunohistochemistry was used to identify uNK (CD56+, CD16−, CD3−). Twenty-nine women with RM and >5% uNK agreed to take 20 mg oral prednisolone daily from day 1 to 21 of their menstrual cycle, when a second biopsy was obtained and analyzed. Main Outcome Measure(s) The percentage of stromal cells that were uNK. The normal range was defined using control samples as <5%. Result(s) Women with RM had significantly more uNK than the controls (P=.008). Prednisolone treatment significantly reduced the number of CD56 cells in the endometrium, from a median of 14% (before) to 9% (after) (P=.0004). Conclusion(s) We have demonstrated that high numbers of uNK in preimplantation endometrium of women with recurrent miscarriage can be reduced with administration of prednisolone.
U2 - 10.1016/j.fertnstert.2005.05.012
DO - 10.1016/j.fertnstert.2005.05.012
M3 - Journal article
VL - 84
SP - 980
EP - 984
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 4
ER -