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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Prolonged low temperature exposure de‐sensitises ABA‐induced stomatal closure in soybean, involving an ethylene‐dependent process
AU - Antonietta, Mariana
AU - de Felipe, Matias
AU - Rothwell, Shane A.
AU - Williams, Tom B.
AU - Skilleter, Patrick
AU - Albacete, Alfonso
AU - Borras, Lucas
AU - Rufino, Mariana C.
AU - Dodd, Ian C.
PY - 2023/7/31
Y1 - 2023/7/31
N2 - Chilling can decrease stomatal sensitivity to abscisic acid (ABA) in some legumes, although hormonal mechanisms involved are unclear. After evaluating leaf gas exchange of 16 European soybean genotypes at 14°C, 6 genotypes representing the range of response were selected. Further experiments combined low (L, 14°C) and high (H, 24°C) temperature exposure from sowing until the unifoliate leaf was visible and L or H temperature until full leaf expansion, to impose four temperature treatments: LL, LH, HL, and HH. Prolonged chilling (LL) substantially decreased leaf water content but increased leaf ethylene evolution and foliar concentrations of the ethylene precursor 1‐aminocyclopropane‐1‐carboxylic acid, indole‐3‐acetic acid, ABA and jasmonic acid. Across genotypes, photosynthesis linearly increased with stomatal conductance (Gs), with photosynthesis of HH plants threefold higher than LL plants at the same Gs. In all treatments except LL, Gs declined with foliar ABA accumulation. Foliar ABA sprays substantially decreased Gs of HH plants, but did not significantly affect LL plants. Thus low temperature compromised stomatal sensitivity to endogenous and exogenous ABA. Applying the ethylene antagonist 1 methyl‐cyclopropene partially reverted excessive stomatal opening of LL plants. Thus, chilling‐induced ethylene accumulation may mediate stomatal insensitivity to ABA, offering chemical opportunities for improving seedling survival in cold environments.
AB - Chilling can decrease stomatal sensitivity to abscisic acid (ABA) in some legumes, although hormonal mechanisms involved are unclear. After evaluating leaf gas exchange of 16 European soybean genotypes at 14°C, 6 genotypes representing the range of response were selected. Further experiments combined low (L, 14°C) and high (H, 24°C) temperature exposure from sowing until the unifoliate leaf was visible and L or H temperature until full leaf expansion, to impose four temperature treatments: LL, LH, HL, and HH. Prolonged chilling (LL) substantially decreased leaf water content but increased leaf ethylene evolution and foliar concentrations of the ethylene precursor 1‐aminocyclopropane‐1‐carboxylic acid, indole‐3‐acetic acid, ABA and jasmonic acid. Across genotypes, photosynthesis linearly increased with stomatal conductance (Gs), with photosynthesis of HH plants threefold higher than LL plants at the same Gs. In all treatments except LL, Gs declined with foliar ABA accumulation. Foliar ABA sprays substantially decreased Gs of HH plants, but did not significantly affect LL plants. Thus low temperature compromised stomatal sensitivity to endogenous and exogenous ABA. Applying the ethylene antagonist 1 methyl‐cyclopropene partially reverted excessive stomatal opening of LL plants. Thus, chilling‐induced ethylene accumulation may mediate stomatal insensitivity to ABA, offering chemical opportunities for improving seedling survival in cold environments.
KW - ORIGINAL ARTICLE
KW - ORIGINAL ARTICLES
KW - abscisic acid
KW - chilling
KW - ethylene
KW - photosynthesis
KW - soybean
KW - stomatal conductance
U2 - 10.1111/pce.14590
DO - 10.1111/pce.14590
M3 - Journal article
VL - 46
SP - 2128
EP - 2141
JO - Plant, Cell & Environment
JF - Plant, Cell & Environment
SN - 0140-7791
IS - 7
ER -