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Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies

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<mark>Journal publication date</mark>30/06/1981
<mark>Journal</mark>Journal of Immunological Methods
Issue number3
Volume43
Number of pages7
Pages (from-to)261-267
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Some examples are given of immunofluorescence with tissue sections and microtubular cytoskeletons of cultured cells where the fluorescent dye propidium iodide (PI) has been used as marker of nuclei. The emission wave length of PI is longer than that of flourescein, making it possible to use several different and commonly available filter combinations. The use of nuclei as positional indicators is often a more suitable method than phase microscopy combined with immunofluorescence because of low background illumination against which morphology is viewed, circumventing the need for often expensive phase optics.