Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies
AU - OCKLEFORD, C D
AU - HSI, B L
AU - WAKELY, J
AU - BADLEY, R A
AU - WHYTE, A
AU - FAULK, W P
PY - 1981/6/30
Y1 - 1981/6/30
N2 - Some examples are given of immunofluorescence with tissue sections and microtubular cytoskeletons of cultured cells where the fluorescent dye propidium iodide (PI) has been used as marker of nuclei. The emission wave length of PI is longer than that of flourescein, making it possible to use several different and commonly available filter combinations. The use of nuclei as positional indicators is often a more suitable method than phase microscopy combined with immunofluorescence because of low background illumination against which morphology is viewed, circumventing the need for often expensive phase optics.
AB - Some examples are given of immunofluorescence with tissue sections and microtubular cytoskeletons of cultured cells where the fluorescent dye propidium iodide (PI) has been used as marker of nuclei. The emission wave length of PI is longer than that of flourescein, making it possible to use several different and commonly available filter combinations. The use of nuclei as positional indicators is often a more suitable method than phase microscopy combined with immunofluorescence because of low background illumination against which morphology is viewed, circumventing the need for often expensive phase optics.
U2 - 10.1016/0022-1759(81)90173-3
DO - 10.1016/0022-1759(81)90173-3
M3 - Journal article
VL - 43
SP - 261
EP - 267
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 1872-7905
IS - 3
ER -