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Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies

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Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies. / OCKLEFORD, C D ; HSI, B L ; WAKELY, J et al.
In: Journal of Immunological Methods, Vol. 43, No. 3, 30.06.1981, p. 261-267.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

OCKLEFORD, CD, HSI, BL, WAKELY, J, BADLEY, RA, WHYTE, A & FAULK, WP 1981, 'Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies', Journal of Immunological Methods, vol. 43, no. 3, pp. 261-267. https://doi.org/10.1016/0022-1759(81)90173-3

APA

OCKLEFORD, C. D., HSI, B. L., WAKELY, J., BADLEY, R. A., WHYTE, A., & FAULK, W. P. (1981). Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies. Journal of Immunological Methods, 43(3), 261-267. https://doi.org/10.1016/0022-1759(81)90173-3

Vancouver

OCKLEFORD CD, HSI BL, WAKELY J, BADLEY RA, WHYTE A, FAULK WP. Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies. Journal of Immunological Methods. 1981 Jun 30;43(3):261-267. doi: 10.1016/0022-1759(81)90173-3

Author

OCKLEFORD, C D ; HSI, B L ; WAKELY, J et al. / Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies. In: Journal of Immunological Methods. 1981 ; Vol. 43, No. 3. pp. 261-267.

Bibtex

@article{067a1fb532be4f14960790eddd301aa0,
title = "Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies",
abstract = "Some examples are given of immunofluorescence with tissue sections and microtubular cytoskeletons of cultured cells where the fluorescent dye propidium iodide (PI) has been used as marker of nuclei. The emission wave length of PI is longer than that of flourescein, making it possible to use several different and commonly available filter combinations. The use of nuclei as positional indicators is often a more suitable method than phase microscopy combined with immunofluorescence because of low background illumination against which morphology is viewed, circumventing the need for often expensive phase optics.",
author = "OCKLEFORD, {C D} and HSI, {B L} and J WAKELY and BADLEY, {R A} and A WHYTE and FAULK, {W P}",
year = "1981",
month = jun,
day = "30",
doi = "10.1016/0022-1759(81)90173-3",
language = "English",
volume = "43",
pages = "261--267",
journal = "Journal of Immunological Methods",
issn = "1872-7905",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies

AU - OCKLEFORD, C D

AU - HSI, B L

AU - WAKELY, J

AU - BADLEY, R A

AU - WHYTE, A

AU - FAULK, W P

PY - 1981/6/30

Y1 - 1981/6/30

N2 - Some examples are given of immunofluorescence with tissue sections and microtubular cytoskeletons of cultured cells where the fluorescent dye propidium iodide (PI) has been used as marker of nuclei. The emission wave length of PI is longer than that of flourescein, making it possible to use several different and commonly available filter combinations. The use of nuclei as positional indicators is often a more suitable method than phase microscopy combined with immunofluorescence because of low background illumination against which morphology is viewed, circumventing the need for often expensive phase optics.

AB - Some examples are given of immunofluorescence with tissue sections and microtubular cytoskeletons of cultured cells where the fluorescent dye propidium iodide (PI) has been used as marker of nuclei. The emission wave length of PI is longer than that of flourescein, making it possible to use several different and commonly available filter combinations. The use of nuclei as positional indicators is often a more suitable method than phase microscopy combined with immunofluorescence because of low background illumination against which morphology is viewed, circumventing the need for often expensive phase optics.

U2 - 10.1016/0022-1759(81)90173-3

DO - 10.1016/0022-1759(81)90173-3

M3 - Journal article

VL - 43

SP - 261

EP - 267

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 1872-7905

IS - 3

ER -