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    Rights statement: This is a post-peer-review, pre-copyedit version of an article published in Trypanosomatids. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-0716-0294-2_10

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Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSNChapter (peer-reviewed)peer-review

Published
Publication date30/03/2020
Host publicationTrypanosomatids: Methods and Protocols
EditorsPaul A.M. Michels, Michael L. Ginger, Dan Zilberstein
Place of PublicationNew York
PublisherHumana Press
Pages125-137
Number of pages13
ISBN (electronic)9781071602942
ISBN (print)9781071602935
<mark>Original language</mark>English

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume2116
ISSN (Print)1064-3745
ISSN (electronic)1940-6029

Abstract

Mass spectrometry based proteomics allows for the identification and quantification of protein and phosphorylation site abundance on a proteome wide scale. Here we describe the metabolic labeling of cultured Trypanosoma brucei cells in either the bloodstream or procyclic life cycle stage using stable isotope labeling of amino acids in cell culture (SILAC), and the production of samples suitable for analysis by liquid chromatography tandem mass spectrometry. The protocols require little specialist equipment, and they typically enable quantification of over 4500 proteins and 9000 phosphorylation sites.

Bibliographic note

This is a post-peer-review, pre-copyedit version of an article published in Trypanosomatids. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-0716-0294-2_10