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    Rights statement: This is a post-peer-review, pre-copyedit version of an article published in Trypanosomatids. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-0716-0294-2_10

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Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

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Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages. / Benz, Corinna; Urbaniak, Mick.

Trypanosomatids: Methods and Protocols. ed. / Paul A.M. Michels; Michael L. Ginger; Dan Zilberstein. New York : Humana Press, 2020. p. 125-137 (Methods in Molecular Biology ; Vol. 2116).

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

Harvard

Benz, C & Urbaniak, M 2020, Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages. in PAM Michels, ML Ginger & D Zilberstein (eds), Trypanosomatids: Methods and Protocols. Methods in Molecular Biology , vol. 2116, Humana Press, New York, pp. 125-137. https://doi.org/10.1007/978-1-0716-0294-2_10

APA

Benz, C., & Urbaniak, M. (2020). Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages. In P. A. M. Michels, M. L. Ginger, & D. Zilberstein (Eds.), Trypanosomatids: Methods and Protocols (pp. 125-137). (Methods in Molecular Biology ; Vol. 2116). Humana Press. https://doi.org/10.1007/978-1-0716-0294-2_10

Vancouver

Benz C, Urbaniak M. Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages. In Michels PAM, Ginger ML, Zilberstein D, editors, Trypanosomatids: Methods and Protocols. New York: Humana Press. 2020. p. 125-137. (Methods in Molecular Biology ). doi: 10.1007/978-1-0716-0294-2_10

Author

Benz, Corinna ; Urbaniak, Mick. / Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages. Trypanosomatids: Methods and Protocols. editor / Paul A.M. Michels ; Michael L. Ginger ; Dan Zilberstein. New York : Humana Press, 2020. pp. 125-137 (Methods in Molecular Biology ).

Bibtex

@inbook{923c71c6d0c2424b9829f50a6380b1e0,
title = "Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages",
abstract = "Mass spectrometry based proteomics allows for the identification and quantification of protein and phosphorylation site abundance on a proteome wide scale. Here we describe the metabolic labeling of cultured Trypanosoma brucei cells in either the bloodstream or procyclic life cycle stage using stable isotope labeling of amino acids in cell culture (SILAC), and the production of samples suitable for analysis by liquid chromatography tandem mass spectrometry. The protocols require little specialist equipment, and they typically enable quantification of over 4500 proteins and 9000 phosphorylation sites.",
author = "Corinna Benz and Mick Urbaniak",
note = "This is a post-peer-review, pre-copyedit version of an article published in Trypanosomatids. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-0716-0294-2_10",
year = "2020",
month = mar,
day = "30",
doi = "10.1007/978-1-0716-0294-2_10",
language = "English",
isbn = "9781071602935",
series = "Methods in Molecular Biology ",
publisher = "Humana Press",
pages = "125--137",
editor = "Michels, {Paul A.M.} and Ginger, {Michael L.} and Dan Zilberstein",
booktitle = "Trypanosomatids",

}

RIS

TY - CHAP

T1 - Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages

AU - Benz, Corinna

AU - Urbaniak, Mick

N1 - This is a post-peer-review, pre-copyedit version of an article published in Trypanosomatids. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-0716-0294-2_10

PY - 2020/3/30

Y1 - 2020/3/30

N2 - Mass spectrometry based proteomics allows for the identification and quantification of protein and phosphorylation site abundance on a proteome wide scale. Here we describe the metabolic labeling of cultured Trypanosoma brucei cells in either the bloodstream or procyclic life cycle stage using stable isotope labeling of amino acids in cell culture (SILAC), and the production of samples suitable for analysis by liquid chromatography tandem mass spectrometry. The protocols require little specialist equipment, and they typically enable quantification of over 4500 proteins and 9000 phosphorylation sites.

AB - Mass spectrometry based proteomics allows for the identification and quantification of protein and phosphorylation site abundance on a proteome wide scale. Here we describe the metabolic labeling of cultured Trypanosoma brucei cells in either the bloodstream or procyclic life cycle stage using stable isotope labeling of amino acids in cell culture (SILAC), and the production of samples suitable for analysis by liquid chromatography tandem mass spectrometry. The protocols require little specialist equipment, and they typically enable quantification of over 4500 proteins and 9000 phosphorylation sites.

U2 - 10.1007/978-1-0716-0294-2_10

DO - 10.1007/978-1-0716-0294-2_10

M3 - Chapter (peer-reviewed)

SN - 9781071602935

T3 - Methods in Molecular Biology

SP - 125

EP - 137

BT - Trypanosomatids

A2 - Michels, Paul A.M.

A2 - Ginger, Michael L.

A2 - Zilberstein, Dan

PB - Humana Press

CY - New York

ER -