Rights statement: This is a post-peer-review, pre-copyedit version of an article published in Trypanosomatids. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-0716-0294-2_10
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Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages. / Benz, Corinna; Urbaniak, Mick.
Trypanosomatids: Methods and Protocols. ed. / Paul A.M. Michels; Michael L. Ginger; Dan Zilberstein. New York : Humana Press, 2020. p. 125-137 (Methods in Molecular Biology ; Vol. 2116).Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review
}
TY - CHAP
T1 - Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages
AU - Benz, Corinna
AU - Urbaniak, Mick
N1 - This is a post-peer-review, pre-copyedit version of an article published in Trypanosomatids. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-0716-0294-2_10
PY - 2020/3/30
Y1 - 2020/3/30
N2 - Mass spectrometry based proteomics allows for the identification and quantification of protein and phosphorylation site abundance on a proteome wide scale. Here we describe the metabolic labeling of cultured Trypanosoma brucei cells in either the bloodstream or procyclic life cycle stage using stable isotope labeling of amino acids in cell culture (SILAC), and the production of samples suitable for analysis by liquid chromatography tandem mass spectrometry. The protocols require little specialist equipment, and they typically enable quantification of over 4500 proteins and 9000 phosphorylation sites.
AB - Mass spectrometry based proteomics allows for the identification and quantification of protein and phosphorylation site abundance on a proteome wide scale. Here we describe the metabolic labeling of cultured Trypanosoma brucei cells in either the bloodstream or procyclic life cycle stage using stable isotope labeling of amino acids in cell culture (SILAC), and the production of samples suitable for analysis by liquid chromatography tandem mass spectrometry. The protocols require little specialist equipment, and they typically enable quantification of over 4500 proteins and 9000 phosphorylation sites.
U2 - 10.1007/978-1-0716-0294-2_10
DO - 10.1007/978-1-0716-0294-2_10
M3 - Chapter (peer-reviewed)
SN - 9781071602935
T3 - Methods in Molecular Biology
SP - 125
EP - 137
BT - Trypanosomatids
A2 - Michels, Paul A.M.
A2 - Ginger, Michael L.
A2 - Zilberstein, Dan
PB - Humana Press
CY - New York
ER -