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Protocol for constructing and characterizing recombinant vectored vaccines for rabies virus

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Protocol for constructing and characterizing recombinant vectored vaccines for rabies virus. / Khalifa, Manar E; Munir, Muhammad.
In: STAR protocols, Vol. 5, No. 4, 103392, 20.12.2024.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Khalifa, ME & Munir, M 2024, 'Protocol for constructing and characterizing recombinant vectored vaccines for rabies virus', STAR protocols, vol. 5, no. 4, 103392. https://doi.org/10.1016/j.xpro.2024.103392

APA

Khalifa, M. E., & Munir, M. (2024). Protocol for constructing and characterizing recombinant vectored vaccines for rabies virus. STAR protocols, 5(4), Article 103392. https://doi.org/10.1016/j.xpro.2024.103392

Vancouver

Khalifa ME, Munir M. Protocol for constructing and characterizing recombinant vectored vaccines for rabies virus. STAR protocols. 2024 Dec 20;5(4):103392. Epub 2024 Oct 17. doi: 10.1016/j.xpro.2024.103392

Author

Khalifa, Manar E ; Munir, Muhammad. / Protocol for constructing and characterizing recombinant vectored vaccines for rabies virus. In: STAR protocols. 2024 ; Vol. 5, No. 4.

Bibtex

@article{2df66622410a48658d5bec4d0745d0d0,
title = "Protocol for constructing and characterizing recombinant vectored vaccines for rabies virus",
abstract = "Recombinant vectored vaccines provide economical, rapidly engineered, and tailored immunization strategies. Here, we present a protocol for constructing and characterizing recombinant vectored vaccines for rabies virus (RV). We describe steps for generating replication-competent vesicular stomatitis virus (rcVSV) by replacing vesicular stomatitis virus (VSV) glycoprotein (G) with RV G gene and for recovering rcVSV by co-transfecting baby hamster kidney (BHK-21) cells with the antigenomic VSV cDNA plasmid (pVSV). We then detail procedures for the functional, structural, and molecular characterization of generated rcVSV-RV-Gs.",
author = "Khalifa, {Manar E} and Muhammad Munir",
year = "2024",
month = dec,
day = "20",
doi = "10.1016/j.xpro.2024.103392",
language = "English",
volume = "5",
journal = "STAR protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Protocol for constructing and characterizing recombinant vectored vaccines for rabies virus

AU - Khalifa, Manar E

AU - Munir, Muhammad

PY - 2024/12/20

Y1 - 2024/12/20

N2 - Recombinant vectored vaccines provide economical, rapidly engineered, and tailored immunization strategies. Here, we present a protocol for constructing and characterizing recombinant vectored vaccines for rabies virus (RV). We describe steps for generating replication-competent vesicular stomatitis virus (rcVSV) by replacing vesicular stomatitis virus (VSV) glycoprotein (G) with RV G gene and for recovering rcVSV by co-transfecting baby hamster kidney (BHK-21) cells with the antigenomic VSV cDNA plasmid (pVSV). We then detail procedures for the functional, structural, and molecular characterization of generated rcVSV-RV-Gs.

AB - Recombinant vectored vaccines provide economical, rapidly engineered, and tailored immunization strategies. Here, we present a protocol for constructing and characterizing recombinant vectored vaccines for rabies virus (RV). We describe steps for generating replication-competent vesicular stomatitis virus (rcVSV) by replacing vesicular stomatitis virus (VSV) glycoprotein (G) with RV G gene and for recovering rcVSV by co-transfecting baby hamster kidney (BHK-21) cells with the antigenomic VSV cDNA plasmid (pVSV). We then detail procedures for the functional, structural, and molecular characterization of generated rcVSV-RV-Gs.

U2 - 10.1016/j.xpro.2024.103392

DO - 10.1016/j.xpro.2024.103392

M3 - Journal article

C2 - 39423123

VL - 5

JO - STAR protocols

JF - STAR protocols

SN - 2666-1667

IS - 4

M1 - 103392

ER -