Home > Research > Publications & Outputs > Protocols from Sales et al. (2020) Rubisco acti...

Research

Associated organisational unit

Text available via DOI:

View graph of relations

Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays

Research output: Other contribution

Published

Standard

Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays. / Sales, Cristina RG; Bernardes Da Silva, Anabela; Carmo-Silva, Elizabete.
protocols.io. 2020, Protocols collection.

Research output: Other contribution

Harvard

Sales, CRG, Bernardes Da Silva, A & Carmo-Silva, E 2020, Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays. protocols.io. https://doi.org/10.17504/protocols.io.bf8djrs6

APA

Sales, C. RG., Bernardes Da Silva, A., & Carmo-Silva, E. (2020, Jun 30). Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays. https://doi.org/10.17504/protocols.io.bf8djrs6

Vancouver

Sales CRG, Bernardes Da Silva A, Carmo-Silva E. Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays. 2020. doi: 10.17504/protocols.io.bf8djrs6

Author

Sales, Cristina RG ; Bernardes Da Silva, Anabela ; Carmo-Silva, Elizabete. / Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays. 2020. protocols.io.

Bibtex

@misc{7a113e0d64a74f5fa7e668890fe73f04,
title = "Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays",
abstract = "This collection of protocols includes four assays for measuring Rubisco activity that are used and discussed in the manuscript {"}Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays{"} (Sales et al. 2020).Three of the protocols are NADH-linked microtiter plate-based assays for Rubisco activity that use alternative coupling enzymes:1. GAPDH-GlyPDH, glyceraldehyde-3-phosphate-dehydrogenase and glycerolphosphate-dehydrogenase;2. PEPC-MDH, phosphoenolpyruvate-carboxylase and malate-dehydrogenase;3. PK-LDH, pyruvate-kinase and lactate-dehydrogenase.The radiometric assay for Rubisco activity measures the incorporation of 14CO2 into 3-phosphoglycerate (3-PGA).The fifth protocol describes the purification of 2,3-bisphosphate-dependent phosphoglycerate mutase (dPGM), which is required for the NADH-linked microtiter plate-based assays 2 (PEPC-MDH) and 3 (PK-LDH).",
author = "Sales, {Cristina RG} and {Bernardes Da Silva}, Anabela and Elizabete Carmo-Silva",
year = "2020",
month = jun,
day = "30",
doi = "10.17504/protocols.io.bf8djrs6",
language = "English",
type = "Other",

}

RIS

TY - GEN

T1 - Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays

AU - Sales, Cristina RG

AU - Bernardes Da Silva, Anabela

AU - Carmo-Silva, Elizabete

PY - 2020/6/30

Y1 - 2020/6/30

N2 - This collection of protocols includes four assays for measuring Rubisco activity that are used and discussed in the manuscript "Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays" (Sales et al. 2020).Three of the protocols are NADH-linked microtiter plate-based assays for Rubisco activity that use alternative coupling enzymes:1. GAPDH-GlyPDH, glyceraldehyde-3-phosphate-dehydrogenase and glycerolphosphate-dehydrogenase;2. PEPC-MDH, phosphoenolpyruvate-carboxylase and malate-dehydrogenase;3. PK-LDH, pyruvate-kinase and lactate-dehydrogenase.The radiometric assay for Rubisco activity measures the incorporation of 14CO2 into 3-phosphoglycerate (3-PGA).The fifth protocol describes the purification of 2,3-bisphosphate-dependent phosphoglycerate mutase (dPGM), which is required for the NADH-linked microtiter plate-based assays 2 (PEPC-MDH) and 3 (PK-LDH).

AB - This collection of protocols includes four assays for measuring Rubisco activity that are used and discussed in the manuscript "Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays" (Sales et al. 2020).Three of the protocols are NADH-linked microtiter plate-based assays for Rubisco activity that use alternative coupling enzymes:1. GAPDH-GlyPDH, glyceraldehyde-3-phosphate-dehydrogenase and glycerolphosphate-dehydrogenase;2. PEPC-MDH, phosphoenolpyruvate-carboxylase and malate-dehydrogenase;3. PK-LDH, pyruvate-kinase and lactate-dehydrogenase.The radiometric assay for Rubisco activity measures the incorporation of 14CO2 into 3-phosphoglycerate (3-PGA).The fifth protocol describes the purification of 2,3-bisphosphate-dependent phosphoglycerate mutase (dPGM), which is required for the NADH-linked microtiter plate-based assays 2 (PEPC-MDH) and 3 (PK-LDH).

U2 - 10.17504/protocols.io.bf8djrs6

DO - 10.17504/protocols.io.bf8djrs6

M3 - Other contribution

CY - protocols.io

ER -