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Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes. / Saunders, Amy; Webb, Louise M C; Janas, Michelle L et al.
In: Blood, Vol. 115, No. 16, 22.04.2010, p. 3249-57.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Saunders, A, Webb, LMC, Janas, ML, Hutchings, A, Pascall, J, Carter, C, Pugh, N, Morgan, G, Turner, M & Butcher, GW 2010, 'Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes', Blood, vol. 115, no. 16, pp. 3249-57. https://doi.org/10.1182/blood-2009-08-237586

APA

Saunders, A., Webb, L. M. C., Janas, M. L., Hutchings, A., Pascall, J., Carter, C., Pugh, N., Morgan, G., Turner, M., & Butcher, G. W. (2010). Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes. Blood, 115(16), 3249-57. https://doi.org/10.1182/blood-2009-08-237586

Vancouver

Saunders A, Webb LMC, Janas ML, Hutchings A, Pascall J, Carter C et al. Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes. Blood. 2010 Apr 22;115(16):3249-57. doi: 10.1182/blood-2009-08-237586

Author

Saunders, Amy ; Webb, Louise M C ; Janas, Michelle L et al. / Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes. In: Blood. 2010 ; Vol. 115, No. 16. pp. 3249-57.

Bibtex

@article{7bd9b616f87a4c5894932e122298b48c,
title = "Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes",
abstract = "The guanosine triphosphatases (GTPases) of the immunity-associated protein (GIMAP) family of putative GTPases has been implicated in the regulation of T-lymphocyte development and survival. A mouse conditional knockout allele was generated for the immune GTPase gene GIMAP1. Homozygous loss of this allele under the influence of the lymphoid-expressed hCD2-iCre recombinase transgene led to severe (> 85%) deficiency of mature T lymphocytes and, unexpectedly, of mature B lymphocytes. By contrast there was little effect of GIMAP1 deletion on immature lymphocytes in either B or T lineages, although in vitro studies showed a shortening of the survival time of both immature and mature CD4(+) single-positive thymocytes. These findings show a vital requirement for GIMAP1 in mature lymphocyte development/survival and draw attention to the nonredundant roles of members of the GIMAP GTPase family in these processes.",
keywords = "Animals, B-Lymphocytes/cytology, Blotting, Western, Cell Differentiation/immunology, Cell Separation, Cell Survival, Flow Cytometry, GTP Phosphohydrolases/metabolism, Mice, Mice, Knockout, Polymerase Chain Reaction, Signal Transduction/immunology, T-Lymphocytes/cytology",
author = "Amy Saunders and Webb, {Louise M C} and Janas, {Michelle L} and Amanda Hutchings and John Pascall and Christine Carter and Nicholas Pugh and Geoff Morgan and Martin Turner and Butcher, {Geoffrey W}",
year = "2010",
month = apr,
day = "22",
doi = "10.1182/blood-2009-08-237586",
language = "English",
volume = "115",
pages = "3249--57",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "16",

}

RIS

TY - JOUR

T1 - Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes

AU - Saunders, Amy

AU - Webb, Louise M C

AU - Janas, Michelle L

AU - Hutchings, Amanda

AU - Pascall, John

AU - Carter, Christine

AU - Pugh, Nicholas

AU - Morgan, Geoff

AU - Turner, Martin

AU - Butcher, Geoffrey W

PY - 2010/4/22

Y1 - 2010/4/22

N2 - The guanosine triphosphatases (GTPases) of the immunity-associated protein (GIMAP) family of putative GTPases has been implicated in the regulation of T-lymphocyte development and survival. A mouse conditional knockout allele was generated for the immune GTPase gene GIMAP1. Homozygous loss of this allele under the influence of the lymphoid-expressed hCD2-iCre recombinase transgene led to severe (> 85%) deficiency of mature T lymphocytes and, unexpectedly, of mature B lymphocytes. By contrast there was little effect of GIMAP1 deletion on immature lymphocytes in either B or T lineages, although in vitro studies showed a shortening of the survival time of both immature and mature CD4(+) single-positive thymocytes. These findings show a vital requirement for GIMAP1 in mature lymphocyte development/survival and draw attention to the nonredundant roles of members of the GIMAP GTPase family in these processes.

AB - The guanosine triphosphatases (GTPases) of the immunity-associated protein (GIMAP) family of putative GTPases has been implicated in the regulation of T-lymphocyte development and survival. A mouse conditional knockout allele was generated for the immune GTPase gene GIMAP1. Homozygous loss of this allele under the influence of the lymphoid-expressed hCD2-iCre recombinase transgene led to severe (> 85%) deficiency of mature T lymphocytes and, unexpectedly, of mature B lymphocytes. By contrast there was little effect of GIMAP1 deletion on immature lymphocytes in either B or T lineages, although in vitro studies showed a shortening of the survival time of both immature and mature CD4(+) single-positive thymocytes. These findings show a vital requirement for GIMAP1 in mature lymphocyte development/survival and draw attention to the nonredundant roles of members of the GIMAP GTPase family in these processes.

KW - Animals

KW - B-Lymphocytes/cytology

KW - Blotting, Western

KW - Cell Differentiation/immunology

KW - Cell Separation

KW - Cell Survival

KW - Flow Cytometry

KW - GTP Phosphohydrolases/metabolism

KW - Mice

KW - Mice, Knockout

KW - Polymerase Chain Reaction

KW - Signal Transduction/immunology

KW - T-Lymphocytes/cytology

U2 - 10.1182/blood-2009-08-237586

DO - 10.1182/blood-2009-08-237586

M3 - Journal article

C2 - 20194894

VL - 115

SP - 3249

EP - 3257

JO - Blood

JF - Blood

SN - 0006-4971

IS - 16

ER -