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    Rights statement: This is the author’s version of a work that was accepted for publication in Osteoarthritis and Cartilage. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Osteoarthritis and Cartilage, 27 (8), 2019 DOI: 10.1016/j.joca.2019.04.012

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Raman Spectroscopy identifies differences in ochronotic and non-ochronotic cartilage: a potential novel technique for monitoring ochronosis

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Raman Spectroscopy identifies differences in ochronotic and non-ochronotic cartilage : a potential novel technique for monitoring ochronosis. / Taylor, Adam Michael; Jenks, Daniel; Kammath, Vishnu; Norman, Brendan; Dillon, Jane ; Gallagher, James; Ranganath, Lakshminarayan; Kerns, Jemma Gillian.

In: Osteoarthritis and Cartilage, Vol. 27, No. 8, 01.08.2019, p. 1244-1251.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Taylor, AM, Jenks, D, Kammath, V, Norman, B, Dillon, J, Gallagher, J, Ranganath, L & Kerns, JG 2019, 'Raman Spectroscopy identifies differences in ochronotic and non-ochronotic cartilage: a potential novel technique for monitoring ochronosis', Osteoarthritis and Cartilage, vol. 27, no. 8, pp. 1244-1251. https://doi.org/10.1016/j.joca.2019.04.012

APA

Taylor, A. M., Jenks, D., Kammath, V., Norman, B., Dillon, J., Gallagher, J., Ranganath, L., & Kerns, J. G. (2019). Raman Spectroscopy identifies differences in ochronotic and non-ochronotic cartilage: a potential novel technique for monitoring ochronosis. Osteoarthritis and Cartilage, 27(8), 1244-1251. https://doi.org/10.1016/j.joca.2019.04.012

Vancouver

Taylor AM, Jenks D, Kammath V, Norman B, Dillon J, Gallagher J et al. Raman Spectroscopy identifies differences in ochronotic and non-ochronotic cartilage: a potential novel technique for monitoring ochronosis. Osteoarthritis and Cartilage. 2019 Aug 1;27(8):1244-1251. https://doi.org/10.1016/j.joca.2019.04.012

Author

Taylor, Adam Michael ; Jenks, Daniel ; Kammath, Vishnu ; Norman, Brendan ; Dillon, Jane ; Gallagher, James ; Ranganath, Lakshminarayan ; Kerns, Jemma Gillian. / Raman Spectroscopy identifies differences in ochronotic and non-ochronotic cartilage : a potential novel technique for monitoring ochronosis. In: Osteoarthritis and Cartilage. 2019 ; Vol. 27, No. 8. pp. 1244-1251.

Bibtex

@article{840993d5c675470aba450feaaed4b224,
title = "Raman Spectroscopy identifies differences in ochronotic and non-ochronotic cartilage: a potential novel technique for monitoring ochronosis",
abstract = "ObjectiveAlkaptonuria (AKU) is a rare, inherited disorder of tyrosine metabolism, where patients are unable to breakdown homogentisic acid (HGA), which increases systemically over time. It presents with a clinical triad of features; HGA in urine, ochronosis of collagenous tissues, and the subsequent ochronotic arthritis of these tissues. In recent years the advance in the understanding of the disease and the potential treatment of the disorder looks promising with the data on the efficacy of nitisinone. However, there are limited methods for the detection and monitoring of ochronosis in vivo, or for treatment monitoring. The study aim was to test the hypothesis that Raman spectra would identify a distinct chemical fingerprint for the non-ochronotic, compared to ochronotic cartilage. Design:Ochronotic and non-ochronotic cartilage from human hips and ears were analysed using Raman spectroscopy. Results:Non-ochronotic cartilage spectra were similar and reproducible and typical of normal articular cartilage. Conversely, the ochronotic cartilage samples were highly fluorescent and displayed limited or no discernible Raman peaks in the spectra, in stark contrast to their non-ochronotic pairs. Interestingly, a novel peak was observed associated with the polymer of HGA in the ochronotic cartilage that was confirmed by analysis of pigment derived from synthetic HGA. Conclusion:This technique reveals novel data on the chemical differences in ochronotic compared with non-ochronotic cartilage, these differences are detectable by a technique that is already generating in vivo data and demonstrates the first possible procedure to monitor the progression of ochronosis in tissues of patients with AKU.",
keywords = "Alkaptonuria, Ochronosis, Arthropathy, Raman Spectroscopy, Osteoarthritis, Cartilage",
author = "Taylor, {Adam Michael} and Daniel Jenks and Vishnu Kammath and Brendan Norman and Jane Dillon and James Gallagher and Lakshminarayan Ranganath and Kerns, {Jemma Gillian}",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Osteoarthritis and Cartilage. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Osteoarthritis and Cartilage, 27 (8), 2019 DOI: 10.1016/j.joca.2019.04.012",
year = "2019",
month = aug,
day = "1",
doi = "10.1016/j.joca.2019.04.012",
language = "English",
volume = "27",
pages = "1244--1251",
journal = "Osteoarthritis and Cartilage",
issn = "1063-4584",
publisher = "W.B. Saunders Ltd",
number = "8",

}

RIS

TY - JOUR

T1 - Raman Spectroscopy identifies differences in ochronotic and non-ochronotic cartilage

T2 - a potential novel technique for monitoring ochronosis

AU - Taylor, Adam Michael

AU - Jenks, Daniel

AU - Kammath, Vishnu

AU - Norman, Brendan

AU - Dillon, Jane

AU - Gallagher, James

AU - Ranganath, Lakshminarayan

AU - Kerns, Jemma Gillian

N1 - This is the author’s version of a work that was accepted for publication in Osteoarthritis and Cartilage. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Osteoarthritis and Cartilage, 27 (8), 2019 DOI: 10.1016/j.joca.2019.04.012

PY - 2019/8/1

Y1 - 2019/8/1

N2 - ObjectiveAlkaptonuria (AKU) is a rare, inherited disorder of tyrosine metabolism, where patients are unable to breakdown homogentisic acid (HGA), which increases systemically over time. It presents with a clinical triad of features; HGA in urine, ochronosis of collagenous tissues, and the subsequent ochronotic arthritis of these tissues. In recent years the advance in the understanding of the disease and the potential treatment of the disorder looks promising with the data on the efficacy of nitisinone. However, there are limited methods for the detection and monitoring of ochronosis in vivo, or for treatment monitoring. The study aim was to test the hypothesis that Raman spectra would identify a distinct chemical fingerprint for the non-ochronotic, compared to ochronotic cartilage. Design:Ochronotic and non-ochronotic cartilage from human hips and ears were analysed using Raman spectroscopy. Results:Non-ochronotic cartilage spectra were similar and reproducible and typical of normal articular cartilage. Conversely, the ochronotic cartilage samples were highly fluorescent and displayed limited or no discernible Raman peaks in the spectra, in stark contrast to their non-ochronotic pairs. Interestingly, a novel peak was observed associated with the polymer of HGA in the ochronotic cartilage that was confirmed by analysis of pigment derived from synthetic HGA. Conclusion:This technique reveals novel data on the chemical differences in ochronotic compared with non-ochronotic cartilage, these differences are detectable by a technique that is already generating in vivo data and demonstrates the first possible procedure to monitor the progression of ochronosis in tissues of patients with AKU.

AB - ObjectiveAlkaptonuria (AKU) is a rare, inherited disorder of tyrosine metabolism, where patients are unable to breakdown homogentisic acid (HGA), which increases systemically over time. It presents with a clinical triad of features; HGA in urine, ochronosis of collagenous tissues, and the subsequent ochronotic arthritis of these tissues. In recent years the advance in the understanding of the disease and the potential treatment of the disorder looks promising with the data on the efficacy of nitisinone. However, there are limited methods for the detection and monitoring of ochronosis in vivo, or for treatment monitoring. The study aim was to test the hypothesis that Raman spectra would identify a distinct chemical fingerprint for the non-ochronotic, compared to ochronotic cartilage. Design:Ochronotic and non-ochronotic cartilage from human hips and ears were analysed using Raman spectroscopy. Results:Non-ochronotic cartilage spectra were similar and reproducible and typical of normal articular cartilage. Conversely, the ochronotic cartilage samples were highly fluorescent and displayed limited or no discernible Raman peaks in the spectra, in stark contrast to their non-ochronotic pairs. Interestingly, a novel peak was observed associated with the polymer of HGA in the ochronotic cartilage that was confirmed by analysis of pigment derived from synthetic HGA. Conclusion:This technique reveals novel data on the chemical differences in ochronotic compared with non-ochronotic cartilage, these differences are detectable by a technique that is already generating in vivo data and demonstrates the first possible procedure to monitor the progression of ochronosis in tissues of patients with AKU.

KW - Alkaptonuria

KW - Ochronosis

KW - Arthropathy

KW - Raman Spectroscopy

KW - Osteoarthritis

KW - Cartilage

U2 - 10.1016/j.joca.2019.04.012

DO - 10.1016/j.joca.2019.04.012

M3 - Journal article

VL - 27

SP - 1244

EP - 1251

JO - Osteoarthritis and Cartilage

JF - Osteoarthritis and Cartilage

SN - 1063-4584

IS - 8

ER -