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Rapid assessment of physiological status in Escherichia coli using fluorescent probes

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Rapid assessment of physiological status in Escherichia coli using fluorescent probes. / Porter, J.; Edwards, C.; Pickup, R. W.
In: Journal of Applied Bacteriology, Vol. 79, No. 4, 31.10.1995, p. 399-408.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Porter, J, Edwards, C & Pickup, RW 1995, 'Rapid assessment of physiological status in Escherichia coli using fluorescent probes', Journal of Applied Bacteriology, vol. 79, no. 4, pp. 399-408. https://doi.org/10.1111/j.1365-2672.1995.tb03154.x

APA

Porter, J., Edwards, C., & Pickup, R. W. (1995). Rapid assessment of physiological status in Escherichia coli using fluorescent probes. Journal of Applied Bacteriology, 79(4), 399-408. https://doi.org/10.1111/j.1365-2672.1995.tb03154.x

Vancouver

Porter J, Edwards C, Pickup RW. Rapid assessment of physiological status in Escherichia coli using fluorescent probes. Journal of Applied Bacteriology. 1995 Oct 31;79(4):399-408. doi: 10.1111/j.1365-2672.1995.tb03154.x

Author

Porter, J. ; Edwards, C. ; Pickup, R. W. / Rapid assessment of physiological status in Escherichia coli using fluorescent probes. In: Journal of Applied Bacteriology. 1995 ; Vol. 79, No. 4. pp. 399-408.

Bibtex

@article{adcb415841204afabd187988a2914997,
title = "Rapid assessment of physiological status in Escherichia coli using fluorescent probes",
abstract = "Rapid and direct viability assessment of Escherichia coli in filtered, sterile lake water was possible using multiparameter flow cytometry. Fluorescent dyes were used as probes for different cellular functions (membrane potential, membrane integrity and intracellular enzyme activity), which were correlated with the ability of the cells to respond to nutrient addition while in a stressed state. Measurement of several criteria circumvented limitations imposed by other methods, and provided extensive evidence for the validity of the methods for monitoring cell viability during adoption of a viable‐but‐non‐culturable state in starved E, coli. Macromolecular staining was concomitantly used to monitor changes in cellular protein, RNA and DNA as additional indicators of physiological status during starvation/stress.",
author = "J. Porter and C. Edwards and Pickup, {R. W.}",
year = "1995",
month = oct,
day = "31",
doi = "10.1111/j.1365-2672.1995.tb03154.x",
language = "English",
volume = "79",
pages = "399--408",
journal = "Journal of Applied Bacteriology",
issn = "0021-8847",
publisher = "Wiley-Blackwell",
number = "4",

}

RIS

TY - JOUR

T1 - Rapid assessment of physiological status in Escherichia coli using fluorescent probes

AU - Porter, J.

AU - Edwards, C.

AU - Pickup, R. W.

PY - 1995/10/31

Y1 - 1995/10/31

N2 - Rapid and direct viability assessment of Escherichia coli in filtered, sterile lake water was possible using multiparameter flow cytometry. Fluorescent dyes were used as probes for different cellular functions (membrane potential, membrane integrity and intracellular enzyme activity), which were correlated with the ability of the cells to respond to nutrient addition while in a stressed state. Measurement of several criteria circumvented limitations imposed by other methods, and provided extensive evidence for the validity of the methods for monitoring cell viability during adoption of a viable‐but‐non‐culturable state in starved E, coli. Macromolecular staining was concomitantly used to monitor changes in cellular protein, RNA and DNA as additional indicators of physiological status during starvation/stress.

AB - Rapid and direct viability assessment of Escherichia coli in filtered, sterile lake water was possible using multiparameter flow cytometry. Fluorescent dyes were used as probes for different cellular functions (membrane potential, membrane integrity and intracellular enzyme activity), which were correlated with the ability of the cells to respond to nutrient addition while in a stressed state. Measurement of several criteria circumvented limitations imposed by other methods, and provided extensive evidence for the validity of the methods for monitoring cell viability during adoption of a viable‐but‐non‐culturable state in starved E, coli. Macromolecular staining was concomitantly used to monitor changes in cellular protein, RNA and DNA as additional indicators of physiological status during starvation/stress.

U2 - 10.1111/j.1365-2672.1995.tb03154.x

DO - 10.1111/j.1365-2672.1995.tb03154.x

M3 - Journal article

C2 - 7592133

AN - SCOPUS:0028832454

VL - 79

SP - 399

EP - 408

JO - Journal of Applied Bacteriology

JF - Journal of Applied Bacteriology

SN - 0021-8847

IS - 4

ER -