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Rapid kinetics of an N-terminal mutant of cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase

Research output: Contribution to Journal/MagazineJournal articlepeer-review

<mark>Journal publication date</mark>1/03/1990
<mark>Journal</mark>Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Issue number3
Number of pages4
Pages (from-to)328-331
Publication StatusPublished
<mark>Original language</mark>English


The transient changes in absorption of visible light upon addition of ribulose 1,5-bisphosphate to Co2+-activated ribulose-1,5-bisphosphate carboxylase/oxygenase were used to show altered catalytic properties of a mutant form of the enzyme from Anacystis nidulans. The mutant form of the enzyme had a modified N-terminus and a 10-fold greater Km for ribulose 1,5-bisphosphate than the natural cyanobacterial enzyme.