Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Rapid kinetics of an N-terminal mutant of cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase
AU - Brändén, R.
AU - Keys, A. J.
AU - Parry, M. A J
PY - 1990/3/1
Y1 - 1990/3/1
N2 - The transient changes in absorption of visible light upon addition of ribulose 1,5-bisphosphate to Co2+-activated ribulose-1,5-bisphosphate carboxylase/oxygenase were used to show altered catalytic properties of a mutant form of the enzyme from Anacystis nidulans. The mutant form of the enzyme had a modified N-terminus and a 10-fold greater Km for ribulose 1,5-bisphosphate than the natural cyanobacterial enzyme.
AB - The transient changes in absorption of visible light upon addition of ribulose 1,5-bisphosphate to Co2+-activated ribulose-1,5-bisphosphate carboxylase/oxygenase were used to show altered catalytic properties of a mutant form of the enzyme from Anacystis nidulans. The mutant form of the enzyme had a modified N-terminus and a 10-fold greater Km for ribulose 1,5-bisphosphate than the natural cyanobacterial enzyme.
KW - Enzyme kinetics
KW - ESR
KW - Mutant
KW - Ribulosebisphosphate carboxylase/oxygenase
UR - http://www.scopus.com/inward/record.url?scp=0025037781&partnerID=8YFLogxK
U2 - 10.1016/0167-4838(90)90033-C
DO - 10.1016/0167-4838(90)90033-C
M3 - Journal article
C2 - 2106915
AN - SCOPUS:0025037781
VL - 1037
SP - 328
EP - 331
JO - Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
JF - Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
SN - 0167-4838
IS - 3
ER -