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Real-world carbon nanoparticle exposures induce brain and gonadal alterations in zebrafish (Danio rerio) as determined by biospectroscopy techniques

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Real-world carbon nanoparticle exposures induce brain and gonadal alterations in zebrafish (Danio rerio) as determined by biospectroscopy techniques. / Li, Junyi; Ying, Guang-Guo; Jones, Kevin C.; Martin, Francis L.

In: Analyst, Vol. 140, No. 8, 21.04.2015, p. 2687-2695.

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@article{340852063149434792101ee13f7f4a67,
title = "Real-world carbon nanoparticle exposures induce brain and gonadal alterations in zebrafish (Danio rerio) as determined by biospectroscopy techniques",
abstract = "Carbon-based nanoparticles (CNPs) have emerged as novel man-made materials with diverse applications, which may present significant risks to organisms. To bridge the gap in our knowledge of nano-toxicology, a number of in vitro or in vivo studies have been carried out. However, toxicity data remains limited. Herein, we employed a biospectroscopy approach to assess CNP-induced effects in zebrafish (Danio rerio). Zebrafish were exposed to Fullerene (C60), long or short multi-walled carbon nanotubes (MWCNTs), or single-walled carbon nanotubes (SWCNTs) for 21 days at two concentrations: 0.1 mg L-1 or 0.001 mg L-1. Following exposure, the brain, gills, gonads and liver from zebrafish were interrogated by attenuated total reflection Fourier-transform infrared (ATR-FTIR) or Raman spectroscopy. Computational analysis was then applied to the acquired infrared (IR) spectra, and distinct biochemical segregations between the exposed tissues vs. control were observed with spectral biomarkers of alterations identified. In addition, lipid-to-protein ratios in all four tissues were calculated by the IR spectra; unsaturated lipid levels in brain and gonad were assessed by Raman spectroscopy. Marked lipid alterations were observed. These findings show that biospectroscopy approaches have the potential to detect CNP-induced biochemical alterations in zebrafish.",
keywords = "RAMAN-SPECTROSCOPY, OXIDATIVE STRESS, ENGINEERED NANOPARTICLES, INFRARED-SPECTROSCOPY, MULTIVARIATE-ANALYSIS, ONCORHYNCHUS-MYKISS, FULLERENE C-60, RAINBOW-TROUT, IN-VITRO, NANOTUBES",
author = "Junyi Li and Guang-Guo Ying and Jones, {Kevin C.} and Martin, {Francis L.}",
note = "This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.",
year = "2015",
month = apr,
day = "21",
doi = "10.1039/c4an02227k",
language = "English",
volume = "140",
pages = "2687--2695",
journal = "Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "8",

}

RIS

TY - JOUR

T1 - Real-world carbon nanoparticle exposures induce brain and gonadal alterations in zebrafish (Danio rerio) as determined by biospectroscopy techniques

AU - Li, Junyi

AU - Ying, Guang-Guo

AU - Jones, Kevin C.

AU - Martin, Francis L.

N1 - This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

PY - 2015/4/21

Y1 - 2015/4/21

N2 - Carbon-based nanoparticles (CNPs) have emerged as novel man-made materials with diverse applications, which may present significant risks to organisms. To bridge the gap in our knowledge of nano-toxicology, a number of in vitro or in vivo studies have been carried out. However, toxicity data remains limited. Herein, we employed a biospectroscopy approach to assess CNP-induced effects in zebrafish (Danio rerio). Zebrafish were exposed to Fullerene (C60), long or short multi-walled carbon nanotubes (MWCNTs), or single-walled carbon nanotubes (SWCNTs) for 21 days at two concentrations: 0.1 mg L-1 or 0.001 mg L-1. Following exposure, the brain, gills, gonads and liver from zebrafish were interrogated by attenuated total reflection Fourier-transform infrared (ATR-FTIR) or Raman spectroscopy. Computational analysis was then applied to the acquired infrared (IR) spectra, and distinct biochemical segregations between the exposed tissues vs. control were observed with spectral biomarkers of alterations identified. In addition, lipid-to-protein ratios in all four tissues were calculated by the IR spectra; unsaturated lipid levels in brain and gonad were assessed by Raman spectroscopy. Marked lipid alterations were observed. These findings show that biospectroscopy approaches have the potential to detect CNP-induced biochemical alterations in zebrafish.

AB - Carbon-based nanoparticles (CNPs) have emerged as novel man-made materials with diverse applications, which may present significant risks to organisms. To bridge the gap in our knowledge of nano-toxicology, a number of in vitro or in vivo studies have been carried out. However, toxicity data remains limited. Herein, we employed a biospectroscopy approach to assess CNP-induced effects in zebrafish (Danio rerio). Zebrafish were exposed to Fullerene (C60), long or short multi-walled carbon nanotubes (MWCNTs), or single-walled carbon nanotubes (SWCNTs) for 21 days at two concentrations: 0.1 mg L-1 or 0.001 mg L-1. Following exposure, the brain, gills, gonads and liver from zebrafish were interrogated by attenuated total reflection Fourier-transform infrared (ATR-FTIR) or Raman spectroscopy. Computational analysis was then applied to the acquired infrared (IR) spectra, and distinct biochemical segregations between the exposed tissues vs. control were observed with spectral biomarkers of alterations identified. In addition, lipid-to-protein ratios in all four tissues were calculated by the IR spectra; unsaturated lipid levels in brain and gonad were assessed by Raman spectroscopy. Marked lipid alterations were observed. These findings show that biospectroscopy approaches have the potential to detect CNP-induced biochemical alterations in zebrafish.

KW - RAMAN-SPECTROSCOPY

KW - OXIDATIVE STRESS

KW - ENGINEERED NANOPARTICLES

KW - INFRARED-SPECTROSCOPY

KW - MULTIVARIATE-ANALYSIS

KW - ONCORHYNCHUS-MYKISS

KW - FULLERENE C-60

KW - RAINBOW-TROUT

KW - IN-VITRO

KW - NANOTUBES

U2 - 10.1039/c4an02227k

DO - 10.1039/c4an02227k

M3 - Journal article

VL - 140

SP - 2687

EP - 2695

JO - Analyst

JF - Analyst

SN - 0003-2654

IS - 8

ER -