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Reproducible bubble-induced acoustic microstreaming for bead disaggregation and immunoassay in microfluidics

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Reproducible bubble-induced acoustic microstreaming for bead disaggregation and immunoassay in microfluidics. / Chen, Huaying; Gao, Yuan; Petkovic, Karolina et al.
In: Microfluidics and Nanofluidics, Vol. 21, 30, 14.02.2017.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Chen, H, Gao, Y, Petkovic, K, Yang, S, Best, M, Du, Y & Zhu, Y 2017, 'Reproducible bubble-induced acoustic microstreaming for bead disaggregation and immunoassay in microfluidics', Microfluidics and Nanofluidics, vol. 21, 30. https://doi.org/10.1007/s10404-017-1870-2

APA

Chen, H., Gao, Y., Petkovic, K., Yang, S., Best, M., Du, Y., & Zhu, Y. (2017). Reproducible bubble-induced acoustic microstreaming for bead disaggregation and immunoassay in microfluidics. Microfluidics and Nanofluidics, 21, Article 30. https://doi.org/10.1007/s10404-017-1870-2

Vancouver

Chen H, Gao Y, Petkovic K, Yang S, Best M, Du Y et al. Reproducible bubble-induced acoustic microstreaming for bead disaggregation and immunoassay in microfluidics. Microfluidics and Nanofluidics. 2017 Feb 14;21:30. doi: 10.1007/s10404-017-1870-2

Author

Chen, Huaying ; Gao, Yuan ; Petkovic, Karolina et al. / Reproducible bubble-induced acoustic microstreaming for bead disaggregation and immunoassay in microfluidics. In: Microfluidics and Nanofluidics. 2017 ; Vol. 21.

Bibtex

@article{a15d1b1a96094a05a29b2d779b5a733d,
title = "Reproducible bubble-induced acoustic microstreaming for bead disaggregation and immunoassay in microfluidics",
abstract = "The bead-based immunoassay requires not only efficient mixing but also good control of bead-surface-area-to-sample-volume ratio to realise accurate and reproducible detection of low concentration samples. This paper reports the development of a microfluidic platform with the reproducible and efficient bubble-induced micromixing for bead disaggregation and immunoassay of prostate-specific antigen (PSA). The platform consists of a microfluidic chip with a microchamber and rectangular traps for capturing air bubbles and a home-made controller to generate sound wave using an external piezo transducer. Methods for reproducible bubble formation and bubble size control during mixing are explored. The influence of driving voltage, PDMS thickness and the substrate material on the mixing efficiency is characterised by mixing a fluorescence dye and a buffer solution. The optimised acoustic microstreaming is able to break clusters with hundreds of beads and homogenise individual beads over the microchamber. Immunoassay with efficient micromixing has been applied to PSA immunoassay with greatly reduced detection time. This study provides a practical guide for the design and development of the bubble-induced acoustic micromixers for bead disaggregation and on-chip immunoassays.",
keywords = "Bubble, Acoustic, Mixing, Piezo, Microstreaming, Immunoassay, PSA, Lifetime, Disaggregation",
author = "Huaying Chen and Yuan Gao and Karolina Petkovic and Sheng Yang and Michael Best and Yanping Du and Yonggang Zhu",
year = "2017",
month = feb,
day = "14",
doi = "10.1007/s10404-017-1870-2",
language = "English",
volume = "21",
journal = "Microfluidics and Nanofluidics",
issn = "1613-4982",
publisher = "Springer Verlag",

}

RIS

TY - JOUR

T1 - Reproducible bubble-induced acoustic microstreaming for bead disaggregation and immunoassay in microfluidics

AU - Chen, Huaying

AU - Gao, Yuan

AU - Petkovic, Karolina

AU - Yang, Sheng

AU - Best, Michael

AU - Du, Yanping

AU - Zhu, Yonggang

PY - 2017/2/14

Y1 - 2017/2/14

N2 - The bead-based immunoassay requires not only efficient mixing but also good control of bead-surface-area-to-sample-volume ratio to realise accurate and reproducible detection of low concentration samples. This paper reports the development of a microfluidic platform with the reproducible and efficient bubble-induced micromixing for bead disaggregation and immunoassay of prostate-specific antigen (PSA). The platform consists of a microfluidic chip with a microchamber and rectangular traps for capturing air bubbles and a home-made controller to generate sound wave using an external piezo transducer. Methods for reproducible bubble formation and bubble size control during mixing are explored. The influence of driving voltage, PDMS thickness and the substrate material on the mixing efficiency is characterised by mixing a fluorescence dye and a buffer solution. The optimised acoustic microstreaming is able to break clusters with hundreds of beads and homogenise individual beads over the microchamber. Immunoassay with efficient micromixing has been applied to PSA immunoassay with greatly reduced detection time. This study provides a practical guide for the design and development of the bubble-induced acoustic micromixers for bead disaggregation and on-chip immunoassays.

AB - The bead-based immunoassay requires not only efficient mixing but also good control of bead-surface-area-to-sample-volume ratio to realise accurate and reproducible detection of low concentration samples. This paper reports the development of a microfluidic platform with the reproducible and efficient bubble-induced micromixing for bead disaggregation and immunoassay of prostate-specific antigen (PSA). The platform consists of a microfluidic chip with a microchamber and rectangular traps for capturing air bubbles and a home-made controller to generate sound wave using an external piezo transducer. Methods for reproducible bubble formation and bubble size control during mixing are explored. The influence of driving voltage, PDMS thickness and the substrate material on the mixing efficiency is characterised by mixing a fluorescence dye and a buffer solution. The optimised acoustic microstreaming is able to break clusters with hundreds of beads and homogenise individual beads over the microchamber. Immunoassay with efficient micromixing has been applied to PSA immunoassay with greatly reduced detection time. This study provides a practical guide for the design and development of the bubble-induced acoustic micromixers for bead disaggregation and on-chip immunoassays.

KW - Bubble

KW - Acoustic

KW - Mixing

KW - Piezo

KW - Microstreaming

KW - Immunoassay

KW - PSA

KW - Lifetime

KW - Disaggregation

U2 - 10.1007/s10404-017-1870-2

DO - 10.1007/s10404-017-1870-2

M3 - Journal article

VL - 21

JO - Microfluidics and Nanofluidics

JF - Microfluidics and Nanofluidics

SN - 1613-4982

M1 - 30

ER -