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Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1. / Budden, Alice M.; Eravci, Murat; Watson, Adam T. et al.
In: eLife, Vol. 12, e78554, 01.09.2023.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Budden, AM, Eravci, M, Watson, AT, Campillo-Funollet, E, Oliver, AW, Naiman, K & Carr, AM 2023, 'Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1', eLife, vol. 12, e78554. https://doi.org/10.7554/ELIFE.78554

APA

Budden, A. M., Eravci, M., Watson, A. T., Campillo-Funollet, E., Oliver, A. W., Naiman, K., & Carr, A. M. (2023). Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1. eLife, 12, Article e78554. https://doi.org/10.7554/ELIFE.78554

Vancouver

Budden AM, Eravci M, Watson AT, Campillo-Funollet E, Oliver AW, Naiman K et al. Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1. eLife. 2023 Sept 1;12:e78554. Epub 2023 Aug 24. doi: 10.7554/ELIFE.78554

Author

Budden, Alice M. ; Eravci, Murat ; Watson, Adam T. et al. / Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1. In: eLife. 2023 ; Vol. 12.

Bibtex

@article{ebf99ff0998342c5ba21eb5032663e38,
title = "Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1",
abstract = "Arrested replication forks, when restarted by homologous recombination, result in error-prone DNA syntheses and non-allelic homologous recombination. Fission yeast RTS1 is a model fork barrier used to probe mechanisms of recombination-dependent restart. RTS1 barrier activity is entirely dependent on the DNA binding protein Rtf1 and partially dependent on a second protein, Rtf2. Human RTF2 was recently implicated in fork restart, leading us to examine fission yeast Rtf2{\textquoteright}s role in more detail. In agreement with previous studies, we observe reduced barrier activity upon rtf2 deletion. However, we identified Rtf2 to be physically associated with mRNA processing and splicing factors and rtf2 deletion to cause increased intron retention. One of the most affected introns resided in the rtf1 transcript. Using an intronless rtf1, we observed no reduction in RFB activity in the absence of Rtf2. Thus, Rtf2 is essential for correct rtf1 splicing to allow optimal RTS1 barrier activity.",
author = "Budden, {Alice M.} and Murat Eravci and Watson, {Adam T.} and Eduard Campillo-Funollet and Oliver, {Antony W.} and Karel Naiman and Carr, {Antony M.}",
note = "Publisher Copyright: {\textcopyright} Budden et al.",
year = "2023",
month = sep,
day = "1",
doi = "10.7554/ELIFE.78554",
language = "English",
volume = "12",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",

}

RIS

TY - JOUR

T1 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1

AU - Budden, Alice M.

AU - Eravci, Murat

AU - Watson, Adam T.

AU - Campillo-Funollet, Eduard

AU - Oliver, Antony W.

AU - Naiman, Karel

AU - Carr, Antony M.

N1 - Publisher Copyright: © Budden et al.

PY - 2023/9/1

Y1 - 2023/9/1

N2 - Arrested replication forks, when restarted by homologous recombination, result in error-prone DNA syntheses and non-allelic homologous recombination. Fission yeast RTS1 is a model fork barrier used to probe mechanisms of recombination-dependent restart. RTS1 barrier activity is entirely dependent on the DNA binding protein Rtf1 and partially dependent on a second protein, Rtf2. Human RTF2 was recently implicated in fork restart, leading us to examine fission yeast Rtf2’s role in more detail. In agreement with previous studies, we observe reduced barrier activity upon rtf2 deletion. However, we identified Rtf2 to be physically associated with mRNA processing and splicing factors and rtf2 deletion to cause increased intron retention. One of the most affected introns resided in the rtf1 transcript. Using an intronless rtf1, we observed no reduction in RFB activity in the absence of Rtf2. Thus, Rtf2 is essential for correct rtf1 splicing to allow optimal RTS1 barrier activity.

AB - Arrested replication forks, when restarted by homologous recombination, result in error-prone DNA syntheses and non-allelic homologous recombination. Fission yeast RTS1 is a model fork barrier used to probe mechanisms of recombination-dependent restart. RTS1 barrier activity is entirely dependent on the DNA binding protein Rtf1 and partially dependent on a second protein, Rtf2. Human RTF2 was recently implicated in fork restart, leading us to examine fission yeast Rtf2’s role in more detail. In agreement with previous studies, we observe reduced barrier activity upon rtf2 deletion. However, we identified Rtf2 to be physically associated with mRNA processing and splicing factors and rtf2 deletion to cause increased intron retention. One of the most affected introns resided in the rtf1 transcript. Using an intronless rtf1, we observed no reduction in RFB activity in the absence of Rtf2. Thus, Rtf2 is essential for correct rtf1 splicing to allow optimal RTS1 barrier activity.

U2 - 10.7554/ELIFE.78554

DO - 10.7554/ELIFE.78554

M3 - Journal article

C2 - 37615341

AN - SCOPUS:85169503431

VL - 12

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e78554

ER -