TY - JOUR

T1 - Study of the Structure of Hyperbranched Polyglycerol Coatings and Their Antibiofouling and Antithrombotic Applications

AU - Moore, Eli

AU - Robson, Alexander J.

AU - Crisp, Amy R.

AU - Cockshell, Michaelia P.

AU - Burzava, Anouck L. S.

AU - Ganesan, Raja

AU - Robinson, Nirmal

AU - Al‐Bataineh, Sameer

AU - Nankivell, Victoria

AU - Sandeman, Lauren

AU - Tondl, Markus

AU - Benveniste, Glen

AU - Finnie, John W.

AU - Psaltis, Peter J.

AU - Martocq, Laurine

AU - Quadrelli, Alessio

AU - Jarvis, Samuel P.

AU - Williams, Craig

AU - Ramage, Gordon

AU - Rehman, Ihtesham U.

AU - Bursill, Christina A.

AU - Simula, Tony

AU - Voelcker, Nicolas H.

AU - Griesser, Hans J.

AU - Short, Robert D

AU - Bonder, Claudine S.

PY - 2024/10/18

Y1 - 2024/10/18

N2 - While blood‐contacting materials are widely deployed in medicine in vascular stents, catheters, and cannulas, devices fail in situ because of thrombosis and restenosis. Furthermore, microbial attachment and biofilm formation is not an uncommon problem for medical devices. Even incremental improvements in hemocompatible materials can provide significant benefits for patients in terms of safety and patency as well as substantial cost savings. Herein, a novel but simple strategy is described for coating a range of medical materials, that can be applied to objects of complex geometry, involving plasma‐grafting of an ultrathin hyperbranched polyglycerol coating (HPG). Plasma activation creates highly reactive surface oxygen moieties that readily react with glycidol. Irrespective of the substrate, coatings are uniform and pinhole free, comprising O─C─O repeats, with HPG chains packing in a fashion that holds reversibly binding proteins at the coating surface. In vitro assays with planar test samples show that HPG prevents platelet adhesion and activation, as well as reducing (>3 log) bacterial attachment and preventing biofilm formation. Ex vivo and preclinical studies show that HPG‐coated nitinol stents do not elicit thrombosis or restenosis, nor complement or neutrophil activation. Subcutaneous implantation of HPG coated disks under the skin of mice shows no evidence of toxicity nor inflammation.

AB - While blood‐contacting materials are widely deployed in medicine in vascular stents, catheters, and cannulas, devices fail in situ because of thrombosis and restenosis. Furthermore, microbial attachment and biofilm formation is not an uncommon problem for medical devices. Even incremental improvements in hemocompatible materials can provide significant benefits for patients in terms of safety and patency as well as substantial cost savings. Herein, a novel but simple strategy is described for coating a range of medical materials, that can be applied to objects of complex geometry, involving plasma‐grafting of an ultrathin hyperbranched polyglycerol coating (HPG). Plasma activation creates highly reactive surface oxygen moieties that readily react with glycidol. Irrespective of the substrate, coatings are uniform and pinhole free, comprising O─C─O repeats, with HPG chains packing in a fashion that holds reversibly binding proteins at the coating surface. In vitro assays with planar test samples show that HPG prevents platelet adhesion and activation, as well as reducing (>3 log) bacterial attachment and preventing biofilm formation. Ex vivo and preclinical studies show that HPG‐coated nitinol stents do not elicit thrombosis or restenosis, nor complement or neutrophil activation. Subcutaneous implantation of HPG coated disks under the skin of mice shows no evidence of toxicity nor inflammation.

KW - hyperbranched polyglycerol

KW - biofilms

KW - X‐ray photoelectron spectroscopy

KW - in‐stent restenosis

KW - peripheral arterial disease

KW - nonfouling

KW - thrombosis

U2 - 10.1002/adhm.202401545

DO - 10.1002/adhm.202401545

M3 - Journal article

VL - 13

JO - Advanced Healthcare Materials

JF - Advanced Healthcare Materials

SN - 2192-2659

IS - 26

M1 - e2401545

ER -