Final published version
Licence: CC BY: Creative Commons Attribution 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Super‐Resolution Imaging and Optomechanical Manipulation Using Optical Nanojet for Nondestructive Single‐Cell Research
AU - Karabchevsky, Alina
AU - Elbaz, Tal
AU - Katiyi, Aviad
AU - Prager, Ofer
AU - Friedman, Alon
PY - 2022/2/2
Y1 - 2022/2/2
N2 - Advanced photonic tools may enable researchers and clinicians to visualize, track, control, and manipulate biological processes at the single-cell level in space and time. Biological systems are complex and highly organized on both spatial and temporal levels. If biological entities are to be studied, perturbed, engineered, or healed, key-players in such systems must be visualized and it is required to track, control, and manipulate them precisely and selectively. To achieve this goal, the engineering of nondestructive tools allows to interrogate and manipulate the function of proteins, pathways, and cells for physicians, enabling the design of “smart materials” that can direct and respond to biological processes. Among the potentially exploitable nondestructive tools, light-based actuation is particularly desirable. It enables high spatial and temporal resolution, dosage control, minimal disturbance to biological systems, and deep tissue penetration. Herein, existing approaches toward the engineering of light-activated tools for the interrogation and manipulation of single-cell processes are overviewed, and the types of studies and types of functions that can be controlled by light are listed. Timely applications, such as studies of inflammation and crossing brain barrier systems—via super-resolution imaging and optomechanical manipulation—are two representative examples of emerging applications so far never addressed.
AB - Advanced photonic tools may enable researchers and clinicians to visualize, track, control, and manipulate biological processes at the single-cell level in space and time. Biological systems are complex and highly organized on both spatial and temporal levels. If biological entities are to be studied, perturbed, engineered, or healed, key-players in such systems must be visualized and it is required to track, control, and manipulate them precisely and selectively. To achieve this goal, the engineering of nondestructive tools allows to interrogate and manipulate the function of proteins, pathways, and cells for physicians, enabling the design of “smart materials” that can direct and respond to biological processes. Among the potentially exploitable nondestructive tools, light-based actuation is particularly desirable. It enables high spatial and temporal resolution, dosage control, minimal disturbance to biological systems, and deep tissue penetration. Herein, existing approaches toward the engineering of light-activated tools for the interrogation and manipulation of single-cell processes are overviewed, and the types of studies and types of functions that can be controlled by light are listed. Timely applications, such as studies of inflammation and crossing brain barrier systems—via super-resolution imaging and optomechanical manipulation—are two representative examples of emerging applications so far never addressed.
U2 - 10.1002/adpr.202100233
DO - 10.1002/adpr.202100233
M3 - Journal article
VL - 3
JO - Advanced Photonics Research
JF - Advanced Photonics Research
SN - 2699-9293
IS - 2
M1 - 2100233
ER -