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    Rights statement: Copyright: © 2014 Fogarty et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Surface-enhanced Raman spectroscopy of the endothelial cell membrane

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Surface-enhanced Raman spectroscopy of the endothelial cell membrane. / Fogarty, Simon W.; Patel, Imran I.; Martin, Francis L. et al.
In: PLoS ONE, Vol. 9, No. 9, e106283, 04.09.2014.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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Fogarty SW, Patel II, Martin FL, Fullwood NJ. Surface-enhanced Raman spectroscopy of the endothelial cell membrane. PLoS ONE. 2014 Sept 4;9(9):e106283. doi: 10.1371/journal.pone.0106283

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Fogarty, Simon W. ; Patel, Imran I. ; Martin, Francis L. et al. / Surface-enhanced Raman spectroscopy of the endothelial cell membrane. In: PLoS ONE. 2014 ; Vol. 9, No. 9.

Bibtex

@article{c76084e31d2f41c199bed2c8889bd53c,
title = "Surface-enhanced Raman spectroscopy of the endothelial cell membrane",
abstract = "We applied surface-enhanced Raman spectroscopy (SERS) to cationic gold-labeled endothelial cells to derive SERS-enhanced spectra of the bimolecular makeup of the plasma membrane. A two-step protocol with cationic charged gold nanoparticles followed by silver-intensification to generate silver nanoparticles on the cell surface was employed. This protocol of post-labelling silver-intensification facilitates the collection of SERS-enhanced spectra from the cell membrane without contribution from conjugated antibodies or other molecules. This approach generated a 100-fold SERS-enhancement of the spectral signal. The SERS spectra exhibited many vibrational peaks that can be assigned to components of the cell membrane. We were able to carry out spectral mapping using some of the enhanced wavenumbers. Significantly, the spectral maps suggest the distribution of some membrane components are was not evenly distributed over the cells plasma membrane. These results provide some possible evidence for the existence of lipid rafts in the plasma membrane and show that SERS has great potential for the study and characterization of cell surfaces.",
author = "Fogarty, {Simon W.} and Patel, {Imran I.} and Martin, {Francis L.} and Fullwood, {Nigel J.}",
note = "Copyright: {\textcopyright} 2014 Fogarty et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2014",
month = sep,
day = "4",
doi = "10.1371/journal.pone.0106283",
language = "English",
volume = "9",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

RIS

TY - JOUR

T1 - Surface-enhanced Raman spectroscopy of the endothelial cell membrane

AU - Fogarty, Simon W.

AU - Patel, Imran I.

AU - Martin, Francis L.

AU - Fullwood, Nigel J.

N1 - Copyright: © 2014 Fogarty et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2014/9/4

Y1 - 2014/9/4

N2 - We applied surface-enhanced Raman spectroscopy (SERS) to cationic gold-labeled endothelial cells to derive SERS-enhanced spectra of the bimolecular makeup of the plasma membrane. A two-step protocol with cationic charged gold nanoparticles followed by silver-intensification to generate silver nanoparticles on the cell surface was employed. This protocol of post-labelling silver-intensification facilitates the collection of SERS-enhanced spectra from the cell membrane without contribution from conjugated antibodies or other molecules. This approach generated a 100-fold SERS-enhancement of the spectral signal. The SERS spectra exhibited many vibrational peaks that can be assigned to components of the cell membrane. We were able to carry out spectral mapping using some of the enhanced wavenumbers. Significantly, the spectral maps suggest the distribution of some membrane components are was not evenly distributed over the cells plasma membrane. These results provide some possible evidence for the existence of lipid rafts in the plasma membrane and show that SERS has great potential for the study and characterization of cell surfaces.

AB - We applied surface-enhanced Raman spectroscopy (SERS) to cationic gold-labeled endothelial cells to derive SERS-enhanced spectra of the bimolecular makeup of the plasma membrane. A two-step protocol with cationic charged gold nanoparticles followed by silver-intensification to generate silver nanoparticles on the cell surface was employed. This protocol of post-labelling silver-intensification facilitates the collection of SERS-enhanced spectra from the cell membrane without contribution from conjugated antibodies or other molecules. This approach generated a 100-fold SERS-enhancement of the spectral signal. The SERS spectra exhibited many vibrational peaks that can be assigned to components of the cell membrane. We were able to carry out spectral mapping using some of the enhanced wavenumbers. Significantly, the spectral maps suggest the distribution of some membrane components are was not evenly distributed over the cells plasma membrane. These results provide some possible evidence for the existence of lipid rafts in the plasma membrane and show that SERS has great potential for the study and characterization of cell surfaces.

U2 - 10.1371/journal.pone.0106283

DO - 10.1371/journal.pone.0106283

M3 - Journal article

C2 - 25188340

VL - 9

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 9

M1 - e106283

ER -