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Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms

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Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms. / Deere, D.; Porter, J.; Pickup, R. W. et al.
In: Journal of Applied Bacteriology, Vol. 81, No. 3, 30.09.1996, p. 309-318.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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Deere D, Porter J, Pickup RW, Edwards C. Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms. Journal of Applied Bacteriology. 1996 Sept 30;81(3):309-318. doi: 10.1111/j.1365-2672.1996.tb04333.x

Author

Deere, D. ; Porter, J. ; Pickup, R. W. et al. / Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms. In: Journal of Applied Bacteriology. 1996 ; Vol. 81, No. 3. pp. 309-318.

Bibtex

@article{7d1ffb9e20d64979a158bde572e278f8,
title = "Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms",
abstract = "The survival of the bacterial fish pathogen Aeromonas salmonicida, and persistence of its DNA, were monitored in aquatic microcosms using selective culture and most probable number PCR. Bacterial cells and naked DNA were released into natural non-sterile microcosms consisting of lake sediment overlayered with lake water. Two different types of surface sediment were used. One was sandy in character, taken from the shoreline whilst the other was a littoral loamy surface mud. Inoculated cells and naked DNA became undetectable from water overlayers within 4 weeks of release. Colony counts of Aer. salmonicida declined below detectable limits after 4 weeks in loamy sediment or 7 weeks in sandy sediment; however, naked DNA and DNA from released cells remained detectable for more than 13 weeks.",
author = "D. Deere and J. Porter and Pickup, {R. W.} and C. Edwards",
year = "1996",
month = sep,
day = "30",
doi = "10.1111/j.1365-2672.1996.tb04333.x",
language = "English",
volume = "81",
pages = "309--318",
journal = "Journal of Applied Bacteriology",
issn = "0021-8847",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms

AU - Deere, D.

AU - Porter, J.

AU - Pickup, R. W.

AU - Edwards, C.

PY - 1996/9/30

Y1 - 1996/9/30

N2 - The survival of the bacterial fish pathogen Aeromonas salmonicida, and persistence of its DNA, were monitored in aquatic microcosms using selective culture and most probable number PCR. Bacterial cells and naked DNA were released into natural non-sterile microcosms consisting of lake sediment overlayered with lake water. Two different types of surface sediment were used. One was sandy in character, taken from the shoreline whilst the other was a littoral loamy surface mud. Inoculated cells and naked DNA became undetectable from water overlayers within 4 weeks of release. Colony counts of Aer. salmonicida declined below detectable limits after 4 weeks in loamy sediment or 7 weeks in sandy sediment; however, naked DNA and DNA from released cells remained detectable for more than 13 weeks.

AB - The survival of the bacterial fish pathogen Aeromonas salmonicida, and persistence of its DNA, were monitored in aquatic microcosms using selective culture and most probable number PCR. Bacterial cells and naked DNA were released into natural non-sterile microcosms consisting of lake sediment overlayered with lake water. Two different types of surface sediment were used. One was sandy in character, taken from the shoreline whilst the other was a littoral loamy surface mud. Inoculated cells and naked DNA became undetectable from water overlayers within 4 weeks of release. Colony counts of Aer. salmonicida declined below detectable limits after 4 weeks in loamy sediment or 7 weeks in sandy sediment; however, naked DNA and DNA from released cells remained detectable for more than 13 weeks.

U2 - 10.1111/j.1365-2672.1996.tb04333.x

DO - 10.1111/j.1365-2672.1996.tb04333.x

M3 - Journal article

AN - SCOPUS:0029825010

VL - 81

SP - 309

EP - 318

JO - Journal of Applied Bacteriology

JF - Journal of Applied Bacteriology

SN - 0021-8847

IS - 3

ER -