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Synovial fluid analysis of MMP-2, MMP-9, and COX-2 as diagnostic markers for naturally occurring septic and aseptic arthritis in horses

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  • S.W. Abdelhaleem
  • M.M. Bashandy
  • S.I. Salem
  • F.A. Torad
  • H.O. AbuBakr
  • M.M. Bayoumi
<mark>Journal publication date</mark>31/08/2021
<mark>Journal</mark>Comparative Clinical Pathology
Number of pages7
Pages (from-to)577-583
Publication StatusPublished
Early online date4/03/21
<mark>Original language</mark>English


The aim of this study was to explore the diagnostic value of matrix metalloproteinase (MMP)-2 and -9, and cyclooxygenase-2 (COX-2) enzymes in the synovial fluid of horses with different forms of arthritis. Thirty-two horses were involved in this study and based on the clinical, radiographic, and synovial fluid examinations, the horses were divided into three groups: control (group I; n = 12), septic arthritis (group II; n = 5), and aseptic arthritis (group III; n = 15). After routine analysis, synovial fluid was used for assessment of MMP-2 and MMP–9 activities by gelatin zymography, and COX-2 relative gene expression by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Synovial fluid gelatin zymography showed significant gelatinolytic activity for MMP-9 in group II. COX-2 exhibited a 50-fold expression in group II and a 4.5-fold expression in group III compared to group I. In conclusion, the results confirm that MMP-9 and COX-2 detection offers an important diagnostic potential for septic and aseptic arthritis in horses.