TY - JOUR

T1 - Synovial fluid analysis of MMP-2, MMP-9, and COX-2 as diagnostic markers for naturally occurring septic and aseptic arthritis in horses

AU - Abdelhaleem, S.W.

AU - Bashandy, M.M.

AU - Salem, S.I.

AU - Torad, F.A.

AU - AbuBakr, H.O.

AU - Bayoumi, M.M.

PY - 2021/8/31

Y1 - 2021/8/31

N2 - The aim of this study was to explore the diagnostic value of matrix metalloproteinase (MMP)-2 and -9, and cyclooxygenase-2 (COX-2) enzymes in the synovial fluid of horses with different forms of arthritis. Thirty-two horses were involved in this study and based on the clinical, radiographic, and synovial fluid examinations, the horses were divided into three groups: control (group I; n = 12), septic arthritis (group II; n = 5), and aseptic arthritis (group III; n = 15). After routine analysis, synovial fluid was used for assessment of MMP-2 and MMP–9 activities by gelatin zymography, and COX-2 relative gene expression by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Synovial fluid gelatin zymography showed significant gelatinolytic activity for MMP-9 in group II. COX-2 exhibited a 50-fold expression in group II and a 4.5-fold expression in group III compared to group I. In conclusion, the results confirm that MMP-9 and COX-2 detection offers an important diagnostic potential for septic and aseptic arthritis in horses.

AB - The aim of this study was to explore the diagnostic value of matrix metalloproteinase (MMP)-2 and -9, and cyclooxygenase-2 (COX-2) enzymes in the synovial fluid of horses with different forms of arthritis. Thirty-two horses were involved in this study and based on the clinical, radiographic, and synovial fluid examinations, the horses were divided into three groups: control (group I; n = 12), septic arthritis (group II; n = 5), and aseptic arthritis (group III; n = 15). After routine analysis, synovial fluid was used for assessment of MMP-2 and MMP–9 activities by gelatin zymography, and COX-2 relative gene expression by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Synovial fluid gelatin zymography showed significant gelatinolytic activity for MMP-9 in group II. COX-2 exhibited a 50-fold expression in group II and a 4.5-fold expression in group III compared to group I. In conclusion, the results confirm that MMP-9 and COX-2 detection offers an important diagnostic potential for septic and aseptic arthritis in horses.

KW - Arthritis

KW - COX-2

KW - Gelatin zymography

KW - Horses

KW - MMP-2

KW - MMP-9

U2 - 10.1007/s00580-021-03226-z

DO - 10.1007/s00580-021-03226-z

M3 - Journal article

VL - 30

SP - 577

EP - 583

JO - Comparative Clinical Pathology

JF - Comparative Clinical Pathology

SN - 1618-5641

ER -