Home > Research > Publications & Outputs > Targeted Knockdown of GDCH in Rice Leads to a P...


Text available via DOI:

View graph of relations

Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice

Research output: Contribution to Journal/MagazineJournal articlepeer-review

  • HsiangChun Lin
  • Shanta Karki
  • Robert A Coe
  • Shaheen Bagha
  • C Paolo Balahadia
  • Julius Ver Sagun
  • Ronald Tapia
  • W Krystler Israel
  • Florencia Montecillo
  • Albert de Luna
  • Florence R Danila
  • Andrea Lazaro
  • Czarina M Realubit
  • Michelle G Acoba
  • Tammy L Sage
  • Susanne von Caemmerer
  • Robert T Furbank
  • Asaph B Cousins
  • Julian M Hibberd
  • W Paul Quick
  • Sarah Covshoff
<mark>Journal publication date</mark>30/05/2016
<mark>Journal</mark>Plant and Cell Physiology
Issue number5
Number of pages14
Pages (from-to)919-32
Publication StatusPublished
Early online date21/02/16
<mark>Original language</mark>English
Externally publishedYes


The glycine decarboxylase complex (GDC) plays a critical role in the photorespiratory C2 cycle of C3 species by recovering carbon following the oxygenation reaction of ribulose-1,5-bisphosphate carboxylase/oxygenase. Loss of GDC from mesophyll cells (MCs) is considered a key early step in the evolution of C4 photosynthesis. To assess the impact of preferentially reducing GDC in rice MCs, we decreased the abundance of OsGDCH (Os10g37180) using an artificial microRNA (amiRNA) driven by a promoter that preferentially drives expression in MCs. GDC H- and P-proteins were undetectable in leaves of gdch lines. Plants exhibited a photorespiratory-deficient phenotype with stunted growth, accelerated leaf senescence, reduced chlorophyll, soluble protein and sugars, and increased glycine accumulation in leaves. Gas exchange measurements indicated an impaired ability to regenerate ribulose 1,5-bisphosphate in photorespiratory conditions. In addition, MCs of gdch lines exhibited a significant reduction in chloroplast area and coverage of the cell wall when grown in air, traits that occur during the later stages of C4 evolution. The presence of these two traits important for C4 photosynthesis and the non-lethal, down-regulation of the photorespiratory C2 cycle positively contribute to efforts to produce a C4 rice prototype.

Bibliographic note

© The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.