Rights statement: This is an Accepted Manuscript of an article published by Taylor & Francis in Soil and Sediment Contamination on 27/11/2016, available online: http://www.tandfonline.com/10.1080/15320383.2017.1264923
Accepted author manuscript, 583 KB, PDF document
Available under license: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License
Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - The dynamic change of microbial communities in crude oil contaminated soils from oilfields in China
AU - Jia, Jianli
AU - Zong, Shuang
AU - Hu, Lei
AU - Shi, Shaohe
AU - Zhai, Xiaobo
AU - Wang, Bingbing
AU - Li, Guanghe
AU - Zhang, Dayi
PY - 2017/2
Y1 - 2017/2
N2 - To study the biodegradability of microbial communities in crude oil contamination, crude oil-contaminated soil samples from different areas of China were collected. Using polyphasic approach, this study explored the dynamic change of the microbial communities during natural accumulation in oilfield and how the constructed bioremediation systems reshape the composition of microbial communities. The abundance of oil-degrading microbes was highest when oil content was 3%–8%. This oil content is potentially optimal for oil-degrading bacteria proliferate. During a ∼12 months natural accumulation, the quantity of oil-degrading microbes increased from 105 to 108 cells/g of soil. A typical sample of Liaohe (LH, oil-contaminated site near Liaohe river, Liaoning Province, China) was remediated for 50 days to investigate the dynamic change of microbial communities. The average FDA (a fluorescein diacetate approach) activities reached 0.25 abs/h·g dry soil in the artificially enhanced repair system, 32% higher than the 0.19 abs/h·g dry soil in natural circumstances. The abundance of oil-degrading microbes increased steadily from 0.001 to 0.068. During remediation treatment, oil content in the soil sample was reduced from 6.0% to 3.7%. GC-MS analysis indicated up to 67% utilization of C10–C20 normal paraffin hydrocarbons, the typical compounds that undergo microbial degradation.
AB - To study the biodegradability of microbial communities in crude oil contamination, crude oil-contaminated soil samples from different areas of China were collected. Using polyphasic approach, this study explored the dynamic change of the microbial communities during natural accumulation in oilfield and how the constructed bioremediation systems reshape the composition of microbial communities. The abundance of oil-degrading microbes was highest when oil content was 3%–8%. This oil content is potentially optimal for oil-degrading bacteria proliferate. During a ∼12 months natural accumulation, the quantity of oil-degrading microbes increased from 105 to 108 cells/g of soil. A typical sample of Liaohe (LH, oil-contaminated site near Liaohe river, Liaoning Province, China) was remediated for 50 days to investigate the dynamic change of microbial communities. The average FDA (a fluorescein diacetate approach) activities reached 0.25 abs/h·g dry soil in the artificially enhanced repair system, 32% higher than the 0.19 abs/h·g dry soil in natural circumstances. The abundance of oil-degrading microbes increased steadily from 0.001 to 0.068. During remediation treatment, oil content in the soil sample was reduced from 6.0% to 3.7%. GC-MS analysis indicated up to 67% utilization of C10–C20 normal paraffin hydrocarbons, the typical compounds that undergo microbial degradation.
KW - Crude oil
KW - oil-contaminated soils
KW - oil-degrading microbes
KW - microbial dynamic change
U2 - 10.1080/15320383.2017.1264923
DO - 10.1080/15320383.2017.1264923
M3 - Journal article
VL - 26
SP - 171
EP - 183
JO - Soil and Sediment Contamination: An International Journal
JF - Soil and Sediment Contamination: An International Journal
SN - 1549-7887
IS - 2
ER -