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The effects of the adrenoceptor agonists phenylephrine and isoproterenol on the intracellular ion concentrations of branchial epithelial-cells of brown trout (Salmo trutta L.).

Research output: Contribution to journalJournal article

Published
  • I. J. Morgan
  • W. T. W. Potts
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<mark>Journal publication date</mark>12/1995
<mark>Journal</mark>Journal of Comparative Physiology B
Issue number6
Volume165
Number of pages6
Pages (from-to)458-463
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Brown trout were fitted with indwelling, intraperitoneal catheters and injected with 4–6 mol · kg-1 of the -receptor agonist phenylephrine or the -receptor agonist isoproterenol. The intracellular concentrations of sodium, chlorine, potassium and phosphorus in the pavement epithelial cells and the mitochondria-rich cells of the branchial epithelium were measured by X-ray microanalysis 1 h after the injection of the adrenoreceptor agonists. Injection with phenylephrine resulted in a significant increase in intracellular chlorine and potassium in mitochondria-rich cells and a significant but relatively smaller increase in chlorine in pavement epithelial cells. Injection with isoproterenol resulted in a significant increase in sodium and chlorine concentration in pavement epithelial cells and a significant decrease in potassium concentration. The only significant effect of isoproterenol injection on mitochondria-rich cells was a decrease in intracellular chlorine concentration. The results suggest that these adrenoreceptor agonists have a direct effect on the influx of Na+ and Cl- across the branchial epithelium. These effects may be a mechanism for acid-base regulation during the severe stress conditions that elicit catecholamine release in vivo. These results corroborate previous studies using X-ray microanalysis which suggested that pavement epithelial cells are the sites of Na+ uptake in freshwater fish whilst Cl- uptake occurs via mitochondria-rich cells.