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The effects of the adrenoceptor agonists phenylephrine and isoproterenol on the intracellular ion concentrations of branchial epithelial-cells of brown trout (Salmo trutta L.).

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The effects of the adrenoceptor agonists phenylephrine and isoproterenol on the intracellular ion concentrations of branchial epithelial-cells of brown trout (Salmo trutta L.). / Morgan, I. J.; Potts, W. T. W.
In: Journal of Comparative Physiology B, Vol. 165, No. 6, 12.1995, p. 458-463.

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@article{42ea8ad205954e5db2a2aa54040f1e3c,
title = "The effects of the adrenoceptor agonists phenylephrine and isoproterenol on the intracellular ion concentrations of branchial epithelial-cells of brown trout (Salmo trutta L.).",
abstract = "Brown trout were fitted with indwelling, intraperitoneal catheters and injected with 4–6 mol · kg-1 of the -receptor agonist phenylephrine or the -receptor agonist isoproterenol. The intracellular concentrations of sodium, chlorine, potassium and phosphorus in the pavement epithelial cells and the mitochondria-rich cells of the branchial epithelium were measured by X-ray microanalysis 1 h after the injection of the adrenoreceptor agonists. Injection with phenylephrine resulted in a significant increase in intracellular chlorine and potassium in mitochondria-rich cells and a significant but relatively smaller increase in chlorine in pavement epithelial cells. Injection with isoproterenol resulted in a significant increase in sodium and chlorine concentration in pavement epithelial cells and a significant decrease in potassium concentration. The only significant effect of isoproterenol injection on mitochondria-rich cells was a decrease in intracellular chlorine concentration. The results suggest that these adrenoreceptor agonists have a direct effect on the influx of Na+ and Cl- across the branchial epithelium. These effects may be a mechanism for acid-base regulation during the severe stress conditions that elicit catecholamine release in vivo. These results corroborate previous studies using X-ray microanalysis which suggested that pavement epithelial cells are the sites of Na+ uptake in freshwater fish whilst Cl- uptake occurs via mitochondria-rich cells.",
keywords = "Gills - X-ray microanalysis - Phenylephrine - Isoproterenol - Trout, Salmo trutta",
author = "Morgan, {I. J.} and Potts, {W. T. W.}",
year = "1995",
month = dec,
doi = "10.1007/BF00261300",
language = "English",
volume = "165",
pages = "458--463",
journal = "Journal of Comparative Physiology B",
issn = "0174-1578",
publisher = "Springer Verlag",
number = "6",

}

RIS

TY - JOUR

T1 - The effects of the adrenoceptor agonists phenylephrine and isoproterenol on the intracellular ion concentrations of branchial epithelial-cells of brown trout (Salmo trutta L.).

AU - Morgan, I. J.

AU - Potts, W. T. W.

PY - 1995/12

Y1 - 1995/12

N2 - Brown trout were fitted with indwelling, intraperitoneal catheters and injected with 4–6 mol · kg-1 of the -receptor agonist phenylephrine or the -receptor agonist isoproterenol. The intracellular concentrations of sodium, chlorine, potassium and phosphorus in the pavement epithelial cells and the mitochondria-rich cells of the branchial epithelium were measured by X-ray microanalysis 1 h after the injection of the adrenoreceptor agonists. Injection with phenylephrine resulted in a significant increase in intracellular chlorine and potassium in mitochondria-rich cells and a significant but relatively smaller increase in chlorine in pavement epithelial cells. Injection with isoproterenol resulted in a significant increase in sodium and chlorine concentration in pavement epithelial cells and a significant decrease in potassium concentration. The only significant effect of isoproterenol injection on mitochondria-rich cells was a decrease in intracellular chlorine concentration. The results suggest that these adrenoreceptor agonists have a direct effect on the influx of Na+ and Cl- across the branchial epithelium. These effects may be a mechanism for acid-base regulation during the severe stress conditions that elicit catecholamine release in vivo. These results corroborate previous studies using X-ray microanalysis which suggested that pavement epithelial cells are the sites of Na+ uptake in freshwater fish whilst Cl- uptake occurs via mitochondria-rich cells.

AB - Brown trout were fitted with indwelling, intraperitoneal catheters and injected with 4–6 mol · kg-1 of the -receptor agonist phenylephrine or the -receptor agonist isoproterenol. The intracellular concentrations of sodium, chlorine, potassium and phosphorus in the pavement epithelial cells and the mitochondria-rich cells of the branchial epithelium were measured by X-ray microanalysis 1 h after the injection of the adrenoreceptor agonists. Injection with phenylephrine resulted in a significant increase in intracellular chlorine and potassium in mitochondria-rich cells and a significant but relatively smaller increase in chlorine in pavement epithelial cells. Injection with isoproterenol resulted in a significant increase in sodium and chlorine concentration in pavement epithelial cells and a significant decrease in potassium concentration. The only significant effect of isoproterenol injection on mitochondria-rich cells was a decrease in intracellular chlorine concentration. The results suggest that these adrenoreceptor agonists have a direct effect on the influx of Na+ and Cl- across the branchial epithelium. These effects may be a mechanism for acid-base regulation during the severe stress conditions that elicit catecholamine release in vivo. These results corroborate previous studies using X-ray microanalysis which suggested that pavement epithelial cells are the sites of Na+ uptake in freshwater fish whilst Cl- uptake occurs via mitochondria-rich cells.

KW - Gills - X-ray microanalysis - Phenylephrine - Isoproterenol - Trout

KW - Salmo trutta

U2 - 10.1007/BF00261300

DO - 10.1007/BF00261300

M3 - Journal article

VL - 165

SP - 458

EP - 463

JO - Journal of Comparative Physiology B

JF - Journal of Comparative Physiology B

SN - 0174-1578

IS - 6

ER -