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The genetics of the repair of 5-azacytidine-mediated DNA damage in the fission yeast Schizosaccharomyces pombe.

Research output: Contribution to journalJournal articlepeer-review

Published
<mark>Journal publication date</mark>06/1996
<mark>Journal</mark>Molecular and General Genetics
Issue number4
Volume251
Number of pages10
Pages (from-to)483-492
Publication StatusPublished
<mark>Original language</mark>English

Abstract

We have recently demonstrated thatSchizosaccharomyces pombe cells treated with the nucleoside analogue 5-azacytidine (5-azaC) require previously characterised G2 checkpoint mechanisms for survival. Here we present a survey of known DNA repair mutations which defines those genes required for survival in the presence of 5-azaC. Using a combination of single-mutant and epistasis analyses we find that the excision, mismatch and recombinational repair pathways are all required in some degree for the repair of 5-azaC-mediated DNA damage. There are distinct differences in the epistatic interactions of several of the repair mutations with respect to 5-azaC-mediated DNA damage relative to UV-mediated DNA damage.