Rights statement: © 2014 Toledo-Ortiz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - The HY5-PIF regulatory module coordinates light and temperature control of photosynthetic gene transcription
AU - Toledo-Ortiz, Gabriela
AU - Johansson, Henrik
AU - Lee, Keun Pyo
AU - Bou-Torrent, Jordi
AU - Stewart, Kelly
AU - Steel, Gavin
AU - Rodríguez-Concepción, Manuel
AU - Halliday, Karen J.
N1 - © 2014 Toledo-Ortiz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2014/6/12
Y1 - 2014/6/12
N2 - The ability to interpret daily and seasonal alterations in light and temperature signals is essential for plant survival. This is particularly important during seedling establishment when the phytochrome photoreceptors activate photosynthetic pigment production for photoautotrophic growth. Phytochromes accomplish this partly through the suppression of phytochrome interacting factors (PIFs), negative regulators of chlorophyll and carotenoid biosynthesis. While the bZIP transcription factor long hypocotyl 5 (HY5), a potent PIF antagonist, promotes photosynthetic pigment accumulation in response to light. Here we demonstrate that by directly targeting a common promoter cis-element (G-box), HY5 and PIFs form a dynamic activation-suppression transcriptional module responsive to light and temperature cues. This antagonistic regulatory module provides a simple, direct mechanism through which environmental change can redirect transcriptional control of genes required for photosynthesis and photoprotection. In the regulation of photopigment biosynthesis genes, HY5 and PIFs do not operate alone, but with the circadian clock. However, sudden changes in light or temperature conditions can trigger changes in HY5 and PIFs abundance that adjust the expression of common target genes to optimise photosynthetic performance and growth.
AB - The ability to interpret daily and seasonal alterations in light and temperature signals is essential for plant survival. This is particularly important during seedling establishment when the phytochrome photoreceptors activate photosynthetic pigment production for photoautotrophic growth. Phytochromes accomplish this partly through the suppression of phytochrome interacting factors (PIFs), negative regulators of chlorophyll and carotenoid biosynthesis. While the bZIP transcription factor long hypocotyl 5 (HY5), a potent PIF antagonist, promotes photosynthetic pigment accumulation in response to light. Here we demonstrate that by directly targeting a common promoter cis-element (G-box), HY5 and PIFs form a dynamic activation-suppression transcriptional module responsive to light and temperature cues. This antagonistic regulatory module provides a simple, direct mechanism through which environmental change can redirect transcriptional control of genes required for photosynthesis and photoprotection. In the regulation of photopigment biosynthesis genes, HY5 and PIFs do not operate alone, but with the circadian clock. However, sudden changes in light or temperature conditions can trigger changes in HY5 and PIFs abundance that adjust the expression of common target genes to optimise photosynthetic performance and growth.
KW - Arabidopsis
KW - Arabidopsis Proteins
KW - Basic Helix-Loop-Helix Transcription Factors
KW - Basic-Leucine Zipper Transcription Factors
KW - Carotenoids
KW - Chlorophyll
KW - G-Box Binding Factors
KW - Gene Expression Regulation, Plant
KW - Nuclear Proteins
KW - Photoperiod
KW - Photosynthesis
KW - Promoter Regions, Genetic
KW - Receptors, Peptide
KW - Seasons
KW - Temperature
KW - Transcription, Genetic
KW - Transcriptional Activation
U2 - 10.1371/journal.pgen.1004416
DO - 10.1371/journal.pgen.1004416
M3 - Journal article
C2 - 24922306
VL - 10
JO - PLoS Genetics
JF - PLoS Genetics
SN - 1553-7390
IS - 6
M1 - e1004416
ER -