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The potential of a niacinamide dominated cosmeceutical formulation on fibroblast activity and wound healing in vitro

Research output: Contribution to Journal/MagazineJournal articlepeer-review

  • Quenton Wessels
  • Etheresia Pretorius
  • Celeste M. Smith
  • Hugo Nel
<mark>Journal publication date</mark>04/2014
<mark>Journal</mark>International Wound Journal
Issue number2
Number of pages7
Pages (from-to)152-158
Publication StatusPublished
Early online date14/08/12
<mark>Original language</mark>English


Knowledge on the intrinsic mechanisms involved in wound healing provides opportunity for various therapeutic strategies. The manipulation of dermal fibroblast proliferation and differentiation might prove to beneficially augment wound healing. This study evaluated the combined effects of niacinamide, l-carnosine, hesperidin and Biofactor HSP® on fibroblast activity. The effects on fibroblast collagen production, cellular proliferation, migration and terminal differentiation were assessed. In addition, the authors determined the effects on in vitro wound healing. The optimal concentrations of actives were determined in vitro. Testing parameters included microscopic morphological cell analysis, cell viability and proliferation determination, calorimetric collagen detection and in vitro wound healing dynamics. Results show that 0·31 mg/ml niacinamide, 0·10 mg/ml l-carnosine, 0·05 mg/ml hesperidin and 5·18 µg/ml Biofactor HSP® proved optimal in vitro. The results show that fibroblast collagen synthesis was increased alongside with cellular migration and proliferation.