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The presence of two complete homologous meta pathway operons on TOL plasmid pWW53.

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The presence of two complete homologous meta pathway operons on TOL plasmid pWW53. / Osborne, D. J.; Pickup, R. W.; Williams, P. A.
In: Journal of General Microbiology, Vol. 134, No. 11, 01.11.1988, p. 2965-2975.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Osborne, DJ, Pickup, RW & Williams, PA 1988, 'The presence of two complete homologous meta pathway operons on TOL plasmid pWW53.', Journal of General Microbiology, vol. 134, no. 11, pp. 2965-2975. https://doi.org/10.1099/00221287-134-11-2965

APA

Osborne, D. J., Pickup, R. W., & Williams, P. A. (1988). The presence of two complete homologous meta pathway operons on TOL plasmid pWW53. Journal of General Microbiology, 134(11), 2965-2975. https://doi.org/10.1099/00221287-134-11-2965

Vancouver

Osborne DJ, Pickup RW, Williams PA. The presence of two complete homologous meta pathway operons on TOL plasmid pWW53. Journal of General Microbiology. 1988 Nov 1;134(11):2965-2975. doi: 10.1099/00221287-134-11-2965

Author

Osborne, D. J. ; Pickup, R. W. ; Williams, P. A. / The presence of two complete homologous meta pathway operons on TOL plasmid pWW53. In: Journal of General Microbiology. 1988 ; Vol. 134, No. 11. pp. 2965-2975.

Bibtex

@article{c72ff156dd63487c9359a57fef6e12a7,
title = "The presence of two complete homologous meta pathway operons on TOL plasmid pWW53.",
abstract = "pWW53 is a 110 kbp catabolic plasmid which encodes the complete pathway for the utilization of toluene and the xylenes. The upper pathway operon xylCAB is located between two homologous but distinct meta pathway operons, xylDLEGF(I,J,K)H, which are in direct repeat. These have each been cloned on large HindIII restriction fragments HA (17.5 kbp) and HB (15.6 kbp), the restriction sites of which have been mapped. During growth of MT53 on benzoate, mutants which have lost the ability to grow on hydrocarbons such as m-xylene (Mxy-) but which retain the ability to grow on their carboxylic acid metabolites such as m-toluate (Mtol+) take over the culture before ultimately being displaced by plasmid-free strains which are Mxy- Mtol-. The plasmids in the Mxy- Mtol+ mutants are formed by a large deletion between homologous regions of the two duplicate meta pathway operons. This causes the loss of the intervening xylCAB operon and the formation of a hybrid xylDLEGF(I, J, K)H operon, starting with the genes originally on HA and terminating with the genes originally on HB.",
author = "Osborne, {D. J.} and Pickup, {R. W.} and Williams, {P. A.}",
year = "1988",
month = nov,
day = "1",
doi = "10.1099/00221287-134-11-2965",
language = "English",
volume = "134",
pages = "2965--2975",
journal = "Journal of General Microbiology",
issn = "0022-1287",
publisher = "Society for General Microbiology",
number = "11",

}

RIS

TY - JOUR

T1 - The presence of two complete homologous meta pathway operons on TOL plasmid pWW53.

AU - Osborne, D. J.

AU - Pickup, R. W.

AU - Williams, P. A.

PY - 1988/11/1

Y1 - 1988/11/1

N2 - pWW53 is a 110 kbp catabolic plasmid which encodes the complete pathway for the utilization of toluene and the xylenes. The upper pathway operon xylCAB is located between two homologous but distinct meta pathway operons, xylDLEGF(I,J,K)H, which are in direct repeat. These have each been cloned on large HindIII restriction fragments HA (17.5 kbp) and HB (15.6 kbp), the restriction sites of which have been mapped. During growth of MT53 on benzoate, mutants which have lost the ability to grow on hydrocarbons such as m-xylene (Mxy-) but which retain the ability to grow on their carboxylic acid metabolites such as m-toluate (Mtol+) take over the culture before ultimately being displaced by plasmid-free strains which are Mxy- Mtol-. The plasmids in the Mxy- Mtol+ mutants are formed by a large deletion between homologous regions of the two duplicate meta pathway operons. This causes the loss of the intervening xylCAB operon and the formation of a hybrid xylDLEGF(I, J, K)H operon, starting with the genes originally on HA and terminating with the genes originally on HB.

AB - pWW53 is a 110 kbp catabolic plasmid which encodes the complete pathway for the utilization of toluene and the xylenes. The upper pathway operon xylCAB is located between two homologous but distinct meta pathway operons, xylDLEGF(I,J,K)H, which are in direct repeat. These have each been cloned on large HindIII restriction fragments HA (17.5 kbp) and HB (15.6 kbp), the restriction sites of which have been mapped. During growth of MT53 on benzoate, mutants which have lost the ability to grow on hydrocarbons such as m-xylene (Mxy-) but which retain the ability to grow on their carboxylic acid metabolites such as m-toluate (Mtol+) take over the culture before ultimately being displaced by plasmid-free strains which are Mxy- Mtol-. The plasmids in the Mxy- Mtol+ mutants are formed by a large deletion between homologous regions of the two duplicate meta pathway operons. This causes the loss of the intervening xylCAB operon and the formation of a hybrid xylDLEGF(I, J, K)H operon, starting with the genes originally on HA and terminating with the genes originally on HB.

U2 - 10.1099/00221287-134-11-2965

DO - 10.1099/00221287-134-11-2965

M3 - Journal article

C2 - 3076178

AN - SCOPUS:0024109524

VL - 134

SP - 2965

EP - 2975

JO - Journal of General Microbiology

JF - Journal of General Microbiology

SN - 0022-1287

IS - 11

ER -