The role of the phospholipase C/inositol 1,4,5-trisphosphate-mediated calcium-mobilizing pathway in guard cell signal transduction

Lancaster Environment Centre

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Centre for Global Eco-innovation

Research output: Contribution to conference - Without ISBN/ISSN › Abstract

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The role of the phospholipase C/inositol 1,4,5-trisphosphate-mediated calcium-mobilizing pathway in guard cell signal transduction. / McAinsh, Martin; Hetherington, Alistair; Leckie, Callum P. et al.
2000. 35-36 Abstract from Plant Biology 2000, San Diego, United States.

Research output: Contribution to conference - Without ISBN/ISSN › Abstract

Bibtex

@conference{c229a086b3ef4fdfb6208df479fbb0c5,

title = "The role of the phospholipase C/inositol 1,4,5-trisphosphate-mediated calcium-mobilizing pathway in guard cell signal transduction",

abstract = "Inositol 1,4,5-trisphosphate (InsP3) is an important component of calcium-based signal transduction pathways in eukaryotic cells. Hydrolysis of phosphatidylinositol 4,5-bisphosphate by phosphoinositide-specific phospholipase C (PI-PLC) generates InsP3, which mobilizes calcium from endomembrane stores, and diacylglycerol. We have used a combined molecular and cell physiological approach to examined the role of the PI-PLC/InsP3-mediated calcium mobilizing pathway in the signal transduction pathway(s) by which stomatal guard cells respond to environmental stimuli such as abscisic acid (ABA) and drought. We have identified a guard cell PI-PLC that is specifically inhibited by an aminosteroid, U-73122; ABA-induced stomatal closure and ABA-induced oscillations in guard cell cytosolic free calcium are both inhibited by U-73122 (Staxen et al., 1999, Proc. Natl. Acad. Sci. USA 96 1779-1784). In addition, microinjection of the competitive InsP3 inhibitor heparin directly into the cytosol of guard cells also inhibits the ABA-induced reduction in guard cell turgor. These data, together with the results of our studies using Nicotiana rustica plants transformed with sense and antisense constructs of the PI-PLC, suggest a role for the PI-PLC/InsP3-mediated calcium-mobilizing pathway in the response of stomata to ABA and drought.",

author = "Martin McAinsh and Alistair Hetherington and Leckie, {Callum P.} and Lewis Mills and Ng, {Carl K-Y.} and Aitken, {F. L.} and Gray, {Julie E.} and Lee Hunt",

year = "2000",

language = "English",

pages = "35--36",

note = "Plant Biology 2000 ; Conference date: 15-07-2000 Through 19-07-2000",

}

RIS

TY - CONF

T1 - The role of the phospholipase C/inositol 1,4,5-trisphosphate-mediated calcium-mobilizing pathway in guard cell signal transduction

AU - McAinsh, Martin

AU - Hetherington, Alistair

AU - Leckie, Callum P.

AU - Mills, Lewis

AU - Ng, Carl K-Y.

AU - Aitken, F. L.

AU - Gray, Julie E.

AU - Hunt, Lee

PY - 2000

Y1 - 2000

N2 - Inositol 1,4,5-trisphosphate (InsP3) is an important component of calcium-based signal transduction pathways in eukaryotic cells. Hydrolysis of phosphatidylinositol 4,5-bisphosphate by phosphoinositide-specific phospholipase C (PI-PLC) generates InsP3, which mobilizes calcium from endomembrane stores, and diacylglycerol. We have used a combined molecular and cell physiological approach to examined the role of the PI-PLC/InsP3-mediated calcium mobilizing pathway in the signal transduction pathway(s) by which stomatal guard cells respond to environmental stimuli such as abscisic acid (ABA) and drought. We have identified a guard cell PI-PLC that is specifically inhibited by an aminosteroid, U-73122; ABA-induced stomatal closure and ABA-induced oscillations in guard cell cytosolic free calcium are both inhibited by U-73122 (Staxen et al., 1999, Proc. Natl. Acad. Sci. USA 96 1779-1784). In addition, microinjection of the competitive InsP3 inhibitor heparin directly into the cytosol of guard cells also inhibits the ABA-induced reduction in guard cell turgor. These data, together with the results of our studies using Nicotiana rustica plants transformed with sense and antisense constructs of the PI-PLC, suggest a role for the PI-PLC/InsP3-mediated calcium-mobilizing pathway in the response of stomata to ABA and drought.

AB - Inositol 1,4,5-trisphosphate (InsP3) is an important component of calcium-based signal transduction pathways in eukaryotic cells. Hydrolysis of phosphatidylinositol 4,5-bisphosphate by phosphoinositide-specific phospholipase C (PI-PLC) generates InsP3, which mobilizes calcium from endomembrane stores, and diacylglycerol. We have used a combined molecular and cell physiological approach to examined the role of the PI-PLC/InsP3-mediated calcium mobilizing pathway in the signal transduction pathway(s) by which stomatal guard cells respond to environmental stimuli such as abscisic acid (ABA) and drought. We have identified a guard cell PI-PLC that is specifically inhibited by an aminosteroid, U-73122; ABA-induced stomatal closure and ABA-induced oscillations in guard cell cytosolic free calcium are both inhibited by U-73122 (Staxen et al., 1999, Proc. Natl. Acad. Sci. USA 96 1779-1784). In addition, microinjection of the competitive InsP3 inhibitor heparin directly into the cytosol of guard cells also inhibits the ABA-induced reduction in guard cell turgor. These data, together with the results of our studies using Nicotiana rustica plants transformed with sense and antisense constructs of the PI-PLC, suggest a role for the PI-PLC/InsP3-mediated calcium-mobilizing pathway in the response of stomata to ABA and drought.

M3 - Abstract

SP - 35

EP - 36

T2 - Plant Biology 2000

Y2 - 15 July 2000 through 19 July 2000

ER -

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