Home > Research > Publications & Outputs > Thermally triggered hydrogel injection into bov...

Research

Links

Text available via DOI:

View graph of relations

Thermally triggered hydrogel injection into bovine intervertebral disc tissue explants induces differentiation of mesenchymal stem cells and restores mechanical function.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Thermally triggered hydrogel injection into bovine intervertebral disc tissue explants induces differentiation of mesenchymal stem cells and restores mechanical function. / AA, Thorpe; Dougill, G; Vickers, L et al.
In: Acta Biomaterialia, Vol. 54, 02.05.2017, p. 212-226.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

AA, T, Dougill, G, Vickers, L, ND, R, Sammon, C, Cooper, G & CL, LM 2017, 'Thermally triggered hydrogel injection into bovine intervertebral disc tissue explants induces differentiation of mesenchymal stem cells and restores mechanical function.', Acta Biomaterialia, vol. 54, pp. 212-226. https://doi.org/10.1016/j.actbio.2017.03.010

APA

AA, T., Dougill, G., Vickers, L., ND, R., Sammon, C., Cooper, G., & CL, L. M. (2017). Thermally triggered hydrogel injection into bovine intervertebral disc tissue explants induces differentiation of mesenchymal stem cells and restores mechanical function. Acta Biomaterialia, 54, 212-226. https://doi.org/10.1016/j.actbio.2017.03.010

Vancouver

AA T, Dougill G, Vickers L, ND R, Sammon C, Cooper G et al. Thermally triggered hydrogel injection into bovine intervertebral disc tissue explants induces differentiation of mesenchymal stem cells and restores mechanical function. Acta Biomaterialia. 2017 May 2;54:212-226. Epub 2017 Mar 8. doi: 10.1016/j.actbio.2017.03.010

Author

AA, Thorpe ; Dougill, G ; Vickers, L et al. / Thermally triggered hydrogel injection into bovine intervertebral disc tissue explants induces differentiation of mesenchymal stem cells and restores mechanical function. In: Acta Biomaterialia. 2017 ; Vol. 54. pp. 212-226.

Bibtex

@article{a826b19a86854622891a7617b2c44a39,
title = "Thermally triggered hydrogel injection into bovine intervertebral disc tissue explants induces differentiation of mesenchymal stem cells and restores mechanical function.",
abstract = "We previously reported a synthetic Laponite{\textregistered} crosslinked pNIPAM-co-DMAc (L-pNIPAM-co-DMAc) hydrogel which promotes differentiation of mesenchymal stem cells (MSCs) to nucleus pulposus (NP) cells without additional growth factors. The clinical success of this hydrogel is dependent on: integration with surrounding tissue; the capacity to restore mechanical function; as well as supporting the viability and differentiation of delivered MSCs. Bovine NP tissue explants were injected with media (control), human MSCs (hMSCs) alone, acellular L-pNIPAM-co-DMAc hydrogel or hMSCs incorporated within the L-pNIPAM-co-DMAc hydrogel and maintained at 5% O2 for 6 weeks. Viability of native NP cells and delivered MSCs was maintained. Furthermore hMSCs delivered via the L-pNIPAM-co-DMAc hydrogel differentiated and produced NP matrix components: aggrecan, collagen type II and chondroitin sulphate, with integration of the hydrogel with native NP tissue. In addition L-pNIPAM-co-DMAc hydrogel injected into collagenase digested bovine discs filled micro and macro fissures, were maintained within the disc during loading and restored IVD stiffness. The mechanical support of the L-pNIPAM-co-DMAc hydrogel, to restore disc height, could provide immediate symptomatic pain relief, whilst the delivery of MSCs over time regenerates the NP extracellular matrix; thus the L-pNIPAM-co-DMAc hydrogel could provide a combined cellular and mechanical repair approach.",
author = "Thorpe AA and G Dougill and L Vickers and Reeves ND and C Sammon and G Cooper and CL, {Le Maitre}",
year = "2017",
month = may,
day = "2",
doi = "10.1016/j.actbio.2017.03.010",
language = "English",
volume = "54",
pages = "212--226",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Thermally triggered hydrogel injection into bovine intervertebral disc tissue explants induces differentiation of mesenchymal stem cells and restores mechanical function.

AU - AA, Thorpe

AU - Dougill, G

AU - Vickers, L

AU - ND, Reeves

AU - Sammon, C

AU - Cooper, G

AU - CL, Le Maitre

PY - 2017/5/2

Y1 - 2017/5/2

N2 - We previously reported a synthetic Laponite® crosslinked pNIPAM-co-DMAc (L-pNIPAM-co-DMAc) hydrogel which promotes differentiation of mesenchymal stem cells (MSCs) to nucleus pulposus (NP) cells without additional growth factors. The clinical success of this hydrogel is dependent on: integration with surrounding tissue; the capacity to restore mechanical function; as well as supporting the viability and differentiation of delivered MSCs. Bovine NP tissue explants were injected with media (control), human MSCs (hMSCs) alone, acellular L-pNIPAM-co-DMAc hydrogel or hMSCs incorporated within the L-pNIPAM-co-DMAc hydrogel and maintained at 5% O2 for 6 weeks. Viability of native NP cells and delivered MSCs was maintained. Furthermore hMSCs delivered via the L-pNIPAM-co-DMAc hydrogel differentiated and produced NP matrix components: aggrecan, collagen type II and chondroitin sulphate, with integration of the hydrogel with native NP tissue. In addition L-pNIPAM-co-DMAc hydrogel injected into collagenase digested bovine discs filled micro and macro fissures, were maintained within the disc during loading and restored IVD stiffness. The mechanical support of the L-pNIPAM-co-DMAc hydrogel, to restore disc height, could provide immediate symptomatic pain relief, whilst the delivery of MSCs over time regenerates the NP extracellular matrix; thus the L-pNIPAM-co-DMAc hydrogel could provide a combined cellular and mechanical repair approach.

AB - We previously reported a synthetic Laponite® crosslinked pNIPAM-co-DMAc (L-pNIPAM-co-DMAc) hydrogel which promotes differentiation of mesenchymal stem cells (MSCs) to nucleus pulposus (NP) cells without additional growth factors. The clinical success of this hydrogel is dependent on: integration with surrounding tissue; the capacity to restore mechanical function; as well as supporting the viability and differentiation of delivered MSCs. Bovine NP tissue explants were injected with media (control), human MSCs (hMSCs) alone, acellular L-pNIPAM-co-DMAc hydrogel or hMSCs incorporated within the L-pNIPAM-co-DMAc hydrogel and maintained at 5% O2 for 6 weeks. Viability of native NP cells and delivered MSCs was maintained. Furthermore hMSCs delivered via the L-pNIPAM-co-DMAc hydrogel differentiated and produced NP matrix components: aggrecan, collagen type II and chondroitin sulphate, with integration of the hydrogel with native NP tissue. In addition L-pNIPAM-co-DMAc hydrogel injected into collagenase digested bovine discs filled micro and macro fissures, were maintained within the disc during loading and restored IVD stiffness. The mechanical support of the L-pNIPAM-co-DMAc hydrogel, to restore disc height, could provide immediate symptomatic pain relief, whilst the delivery of MSCs over time regenerates the NP extracellular matrix; thus the L-pNIPAM-co-DMAc hydrogel could provide a combined cellular and mechanical repair approach.

UR - http://europepmc.org/abstract/med/28285075

U2 - 10.1016/j.actbio.2017.03.010

DO - 10.1016/j.actbio.2017.03.010

M3 - Journal article

C2 - 28285075

VL - 54

SP - 212

EP - 226

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

ER -